Xylooligosaccharides production by commercial enzyme mixture from agricultural wastes and their prebiotic and antioxidant potential

Several techniques involving enzymes as alternatives to rumen fluid inin vitrostudies have been proposed. However, high cost, ill-defined characterisation and high variation among enzyme preparations and batches have discouraged their use. In addition, most studies have aimed at determining dry matter degradability (DMD) at a fixed time, commonly 48 h. Consequently little information has been published concerning the DM degradation dynamics of forages incubated with enzymes. Therefore the objective of the present study was to compare the ability of a commercial enzyme mixture to describe the fermentation dynamics of two contrasting forages, using the ANKOMin vitrofermentation system (Daisy II, ANKOM Co, USA).

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ISOLASI DAN KULTUR PROTOPLAS RUMPUT LAUT Kappaphycus alvarezii DI LABORATORIUM

Jurnal Riset Akuakultur ◽

10.15578/jra.2.3.2007.399-405 ◽

2007 ◽

Vol 2 (3) ◽

pp. 399

Author(s):

Emma Suryati ◽

Andi Tenriulo ◽

Sri Rejeki Hesti Mulyaningrum

Keyword(s):

Evans Blue ◽

Sea Water ◽

Culture Media ◽

Kappaphycus Alvarezii ◽

Commercial Enzyme ◽

Enzyme Mixture ◽

Viable Protoplast ◽

D 12 ◽

Macro Algae ◽

Stimulating Hormone

Isolasi protoplas rumput laut K. alvarezii, telah dilakukan dalam rangka penyiapan protoplas untuk penyilangan melalui fusi protoplas. Metode yang digunakan antara lain melalui cara kimia dengan melisis tallus rumput laut dengan campuran enzim komersial, kemudian enzim yang berasal dari viscera keong mas baik yang segar maupun yang beku, dengan media kultur yang digunakan pada pemeliharaan makro algae antara lain Conwy, PES, dan air laut steril. Tallus rumput laut yang digunakan berasal dari bagian pangkal, tengah dan ujung. Protoplas yang hidup diuji menggunakan evans blue 0,1%, hormon perangsang tumbuh yang digunakan pada media pertumbuhan antara lain auxin, IAA, dan Kinetin. Pengamatan dilakukan terhadap jumlah protoplas hidup, pertumbuhan, dan sintasan. Hasil percobaan memperlihatkan bahwa enzim yang paling baik digunakan adalah campuran enzim komersial dengan media kultur Conwy dengan jumlah protoplas mencapai 19,8 x 106 sel/mL, bagian tallus yang paling baik adalah bagian pangkal berkisar antara 8,1x106 hingga 18,8 x 106 sel/ mL. Perangsang tumbuh yang paling baik adalah auxin. Filamen terbentuk setelah 5 hari dengan fotoperiod L:D=12:12.Isolation of seaweed’s protoplast Kappaphycus alvarezii had been done to provide protoplast for crossbreeding purpose by protoplast fusion. The method was chemically done by lyses of tallus used commercial enzyme mixture, enzyme from viscera of snail both fresh and frozen, culture media were Conwy (CW), PES, and sterile sea water (SSW) which were used to maintain the macro algae. Part of used tallus were upper, middle and tip of tallus. The viable protoplast was examined by using 0.1% evans blue and the growth-stimulating hormone were auxin, IAA, and Kinetin. Observation was concerned to the amount of viable protoplast, the growth, and the long live. Result showed that the best enzyme was commercial enzyme mixture with Conwy as the best culture media, provided protoplast until 19.8 x 106 cell/mL. The greatest protoplast content was in upper part of tallus, it could provide protoplast about 8.1 x 106 cell/mL until 18.8 x 106 cell/mL, and the best growth-stimulating hormone was auxin. Filament was formed after 5 days with photoperiod L: D=12:12.

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