Metabolizable energy in corn is greater than in hybrid rye when fed to gestating sows, but exogenous enzymes did not increase energy digestibility

The metabolizable energy (ME) in corn was greater (P < 0.05) than in hybrid rye when fed to sows in mid-gestation, although the difference between the two grains was less than the difference previously observed in growing pigs. An exogenous enzyme mixture did not increase apparent total tract digestibility of dry matter or gross energy in corn or hybrid rye fed to gestating sows. Likewise, digestible energy and ME in corn and hybrid rye were not increased by the enzymes.

Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass

Staphylotrichum longicolleum FW57 (DSM105789) is a prolific chitinolytic fungus isolated from wood, with a chitinase activity of 0.11 ± 0.01 U/mg. We selected this strain for genome sequencing and annotation, and compiled its growth characteristics on four different chitinous substrates as well as two agro-industrial waste products. We found that the enzymatic mixture secreted by FW57 was not only able to digest pre-treated sugarcane bagasse, but also untreated sugarcane bagasse and maize leaves. The efficiency was comparable to a commercial enzymatic cocktail, highlighting the potential of the S. longicolleum enzyme mixture as an alternative pretreatment method. To further characterize the enzymes, which efficiently digested polymers such as cellulose, hemicellulose, pectin, starch, and lignin, we performed in-depth mass spectrometry-based secretome analysis using tryptic peptides from in-gel and in-solution digestions. Depending on the growth conditions, we were able to detect from 442 to 1092 proteins, which were annotated to identify from 134 to 224 putative carbohydrate-active enzymes (CAZymes) in five different families: glycoside hydrolases, auxiliary activities, carbohydrate esterases, polysaccharide lyases, glycosyl transferases, and proteins containing a carbohydrate-binding module, as well as combinations thereof. The FW57 enzyme mixture could be used to replace commercial enzyme cocktails for the digestion of agro-residual substrates.

Potential of an Enzyme Mixture of Glucose Oxidase, Glucosyl Transferase, and Fructosyl Transferase as an Antidiabetic Medicine

An enzyme mixture (EM) of glucose oxidase, glucosyl transferase, and fructosyl transferase can regulate glucose absorption into the body by converting carbohydrates in food to indigestible oligosaccharides. We evaluated the antidiabetic effects of repeated oral administration of EM in db/db mice. Seven-week-old db/db mice were divided into control, voglibose, and EM groups. Drugs were administered orally mixed with limited feed for one month. Glucose levels were measured every week. A meal tolerance test was conducted after overnight fasting, before the mice were sacrificed. There were no differences in body weight or food intake between the groups. EM treatment reduced blood glucose levels compared with those in the control group. Blood glucose levels during the meal tolerance test were significantly lower in the EM group than those in the control group. A significant decrease in triglyceride level and a tendency for decreased low-density lipoprotein were observed in the EM group compared with in the control group. The Bacteroidetes-to-Firmicutes ratio was higher in the EM group than that in the control group. EM may be useful for people at risk of hyperglycemia or diabetes who need to safely regulate their blood glucose levels. EM may also improve lipid and gut microbiota profiles.

Impact Analysis in Addition of Amylase Enzyme and Cellulose Enzyme Mixture toward Biogas Production from Rice Husk Waste using Solid State Anaerobic Digestion (SS-AD) Method

This research intend to analyze effect of concentrated amylase enzyme and cellulose enzyme, production rate and quality of biogas produced from rice husk waste. The rice husk was given chemical pretreatment by soaking it into NaOH 6 % for 24 hours. Then washed using water so that the pH became normal then dried under the sun before put it inside the reactor with the other enzyme, rumen and urea. The reactor that used has volume of 200ml and has total of 18 reactor with each variable has 2 reactor. Variance of concentrated enzyme that used are 9%, 12%, 15% and 18%. Biogas measurement done every 2 days for 60 days. Result from this research show that addition mixture of amylase enzyme and cellulose enzyme increase yield of biogas that produced. Because yield that produce from reactor with addition of 9%, 12%, 15%, 18% enzyme consecutively are 604 ml, 935 ml, 1041,5 ml and 2922,5 ml. whereas the reactor that not given enzyme only has biogas yield of 115 ml. the highest production rate occur on reactor that were given 18% of enzyme mixture with the production constant rate of 5,39 ml/(grTS day), maximum biogas production amount of 68,53 ml/grTS and the minimum time for biogas to formed is 0,92 days

Rapid and Enhanced Liquefaction of Pulp from Mango (Mangifera indica L.) cv. Totapuri Using Ultrasound-Assisted Enzyme Pretreatment

The effect of ultrasound and enzyme pretreatment (with pectinase, amylase, and cellulase) on the physicochemical properties (yield, viscosity, total soluble solids, and total phenolics) of mango juice was evaluated through a set of six experiments. Ultrasonication treatment alone showed no influence on juice yield (54.6 ± 1.1%). However, the combined uses of ultrasonication with a pectinase or the enzyme mixture significantly increased the yield (94.1 ± 1.4% and 80.0 ± 2.1%, respectively) and decreased the enzyme pretreatment time (from 2 h to 1 h). Pectinase treatment assisted by ultrasonication was more effective with regard to juice yield, viscosity reduction, and the clarity of the juice than the enzyme mixture treatment with ultrasonication. Ultrasonication alone significantly increased the amount of total phenolics (65.5 ± 1.0 mg/100 mL) and showed a slight reduction of viscosity and improvement of clarity compared to the control.