Antioxidant potential of Gossypium hirsutum L. (Malvaceae) using DPPH, ferric reducing antioxidant power, β-carotene bleaching and bioautography assays

The antioxidant activity and bioactive compounds of different blackberry fruit genotypes from the major Brazilian producer region (three cultivars and four selections) were evaluated and compared to the Cherokee cultivar. Phenolic and anthocyanic extracts were obtained and evaluated for each fruit genotype. The phenolic extracts of selections 02/96 and 07/001 presented higher antioxidant activity than those of cultivars in most assays. This activity was partially correlated to the higher amount of total phenolics in these samples. Thus, the phenolic compounds are probably the major responsible for the antioxidant activity in the diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH), ferric reducing antioxidant power (FRAP) assay and thiobarbituric acid reactive substances (TBARS) assay. Quercetin seems to be responsible for the antioxidant activity of blackberry phenolic extracts in the β-carotene bleaching assay. Concerning anthocyanic extracts, the selection 02/96 and Cherokee cultivar from harvest 2007 had higher antioxidant activity than the other genotypes in most assays. Anthocyanins appear to be the major responsible for the antioxidant activity of anthocyanic extracts in the DPPH and FRAP assays, although ascorbic acid also contributed to the DPPH antioxidant activity. Selection 02/96 appears to have higher antioxidant activity than the commercial cultivars cultivated in the southern Brazil and appears to be promising for nutritional and health purposes.

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Influence of Postharvest Temperatures on Carotenoid Biosynthesis and Phytochemicals in Mature Green Chili (Capsicum annuum L.)

Antioxidants ◽

10.3390/antiox9030203 ◽

2020 ◽

Vol 9 (3) ◽

pp. 203

Author(s):

Wissanee Pola ◽

Sumiko Sugaya ◽

Songsin Photchanachai

Keyword(s):

Incubation Temperature ◽

Total Phenolics ◽

Carotenoid Biosynthesis ◽

Temperature Dependent ◽

High Accumulation ◽

Antioxidant Power ◽

Ripening Process ◽

Red Color ◽

Ferric Reducing Antioxidant Power ◽

Β Carotene

An intense red color appearance in hot chili is what industry commonly demands. The harvested mature green “Takanotsume” chili, a popular cultivar in Japan, incubated at 20 and 30 °C is investigated. At 30 °C, the chili rapidly degraded chlorophylls and obtained an intense red color, but presented an orange–red color at 20 °C. The sample showed higher carotenoid accumulations at 30 °C, along with significantly upregulated carotenoid biosynthesis-related genes—phytoene synthase (Psy), lycopene-β-cyclase (Lcyb), β-carotene hydroxylase (CrtZ), and capsanthin/capsorubin synthase (Ccs)—during the experiment. While the expression of the Ccs gene was reduced, there was a 5.5-fold upregulation of the Psy gene at the end of incubation. At 20 °C, the Psy gene was downregulated. These observations suggest that the expression of individual genes is temperature-dependent, and these would affect specific carotenoid compounds. The antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl; DPPH and ferric-reducing antioxidant power; FRAP) values had no difference between temperatures; the higher content of total phenolics and vitamin C presented in the chili at 30 °C probably corresponds to the advanced ripening process. Thus, 30 °C is the recommended incubation temperature for mature green chili to achieve the industry-demanded intense red color and high accumulation of phytochemicals.

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First Evaluation of the Biologically Active Substances and Antioxidant Potential of Regrowth Velvet Antler by means of Multiple Biochemical Assays

Journal of Chemistry ◽

10.1155/2015/975292 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Yujiao Tang ◽

Byong-Tae Jeon ◽

Yanmei Wang ◽

Eun-Ju Choi ◽

Pyo-Jam Park ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Biologically Active Substances ◽

Biologically Active ◽

Antioxidant Potential ◽

Antioxidant Power ◽

Biochemical Assays ◽

Velvet Antler ◽

Ferric Reducing Antioxidant Power ◽

Active Substances

We investigated the biologically active substances contained in RVA (regrowth velvet antler) by comparing the composition of biologically active substances and antioxidant potential of different antler segments. RVA was subjected to extraction using DW (distilled water). RVA was divided into 3 segments: T-RVA (top RVA), M-RVA (middle RVA), and B-RVA (base RVA). The T-RVA section possessed the greatest amounts of uronic acid (36.251 mg/g), sulfated GAGs (sulfated glycosaminoglycans) (555.76 mg/g), sialic acid (111.276 mg/g), uridine (0.957 mg/g), uracil (1.084 mg/g), and hypoxanthine (1.2631 mg/g). In addition, the T-RVA section possessed the strongest antioxidant capacity as determined by DPPH, H2O2(hydrogen peroxide), hydroxyl, and ABTS (2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate) radical scavenging activity as well as FRAP (ferric reducing antioxidant power) and ORAC (oxygen radical absorbance capacity). The values of those were 53.44, 23.09, 34.12, 60.31, and 35.81 TE/μM at 1 mg/mL and 113.57 TE/μM at 20 μg/mL. These results indicate that the T-RVA section possesses the greatest amount of biologically active substances and highest antioxidant potential. This is the first report on the biologically active substances and antioxidant potential of RVA.

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Promising Antioxidant and Anticorrosion Activities of Mild Steel in 1.0 M Hydrochloric Acid Solution by Withania frutescens L. Essential Oil

Frontiers in Chemistry ◽

10.3389/fchem.2021.739273 ◽

2021 ◽

Vol 9 ◽

Author(s):

Abdelfattah El moussaoui ◽

Mariya Kadiri ◽

Mohammed Bourhia ◽

Abdelkrim Agour ◽

Ahmad Mohammad Salamatullah ◽

...

Keyword(s):

Essential Oil ◽

Mild Steel ◽

Electrochemical Impedance ◽

Antioxidant Activities ◽

Polarization Curves ◽

Antioxidant Power ◽

Flame Ionization ◽

Total Antioxidant ◽

Ferric Reducing Antioxidant Power ◽

Β Carotene

The present study was conducted to evaluate the anticorrosive and antioxidant activities of essential oil from Withania frutescens L. In the present study, the extraction of Withania frutescens L. essential oil (Wf-EO) was conducted using hydrodistillation before being characterized by gas chromatographic analysis (GC/MS) and flame ionization detector (GC/FID). Four bioassays were used for antioxidant testing including 2,2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), and β-carotene bleaching. The inhibiting effect of Wf-EO on the corrosion behavior of mild steel in 1.0 M HCl was conducted by using polarization curves and electrochemical impedance spectroscopy techniques. The yield of Wf-EO was 0.46% including 175 compounds identified by GC-MS. The oil was mostly constituted of camphor (37.86%), followed by thujone (26.47%), carvacrol (6.84%), eucalyptol (3.18%), and linalool (2.20%). The anti–free radical activity of Wf-EO was 34.41 ± 0.91 μg/ml (DPPH), 9.67 ± 0.15 mg/ml (FRAP), 3.78 ± 0.41 mg AAE/g (TAC), and 89.94 ± 1.44% (β-carotene). The Wf-EO showed potent antioxidant activity in all bioassays used for testing. The anticorrosion activity, polarization curves as well as EIS diagrams indicated that the Wf-EO exhibited anticorrosive properties and reacted as a suitable corrosion inhibitor in an acidic medium.

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Vacuum evaporation and nitrogen-assisted deodorization affects the antioxidant capacity in the olein fraction of red palm oil and its emulsion products

F1000Research ◽

10.12688/f1000research.16545.1 ◽

2018 ◽

Vol 7 ◽

pp. 1729

Author(s):

Bohari Bohari ◽

Muhammad Muhadir ◽

Anton Rahmadi

Keyword(s):

Antioxidant Capacity ◽

Palm Oil ◽

Vacuum Evaporation ◽

Butylated Hydroxytoluene ◽

Antioxidant Power ◽

Red Palm Oil ◽

Dragon Fruit ◽

Ferric Reducing Antioxidant Power ◽

Β Carotene ◽

Olein Fraction

Background: Deodorization of the olein fraction of red palm oil (OFRP) determines not only the taste of a multivitamin emulsion but also its antioxidant capacity. The emulsion product was formulated from OFRP, pumpkin juice (PJ), and dragon fruit juice (DFJ). This study aimed to optimize vacuum evaporation and nitrogen-assisted deodorizations of OFRP, observing levels of β-carotene, α-tocopherol, inhibition percentage of ABTS reduction, and ferric reducing antioxidant power (FRAP) activity. Methods: The deodorizations observed were vacuum evaporation in four conditions: (1) 90°C, 80±5 mmHg, (2) 100°C, 80±5 mmHg, (3) 90°C, 100±5 mmHg, (4) 100°C, 100±5 mmHg, and nitrogen-assisted in two flow durations: (1) 15 min and (2) 30 min. β-carotene, α-tocopherol, and butylated hydroxytoluene (BHT) were employed as standards. Results: The deodorized OFRP had fewer than 2% free fatty acids (FFA), lower than 3% peroxide value (PV), and lower than 4% acidic value (AV). Fluctuations of the β-carotene and α-tocopherol concentrations were observed in the deodorized OFRP. The final emulsion product had β-carotene of 259.9±1.4 to 271.7±2.4 ppm and α-tocopherol of 36.36±0.20 to 39.12±0.20 ppm. The total betacyanin of the emulsions were ±25% than DFJ. The emulsions had 22.93 to 32.11% of ABTS reduction inhibitory activity of the BHT activity and FRAP activity of 16.54±0.19 to 17.69±0.67 mM FeSO4•7H2O. Conclusions: The best vacuum evaporation optimized at 90 °C, 100±5 mmHg, 60 RPM for 1 hour. The best nitrogen-assisted deodorization was at 85±3°C and 1 l/minute of nitrogen for 15 minutes. The deodorization process affected the antioxidant activity of OFRP and emulsions.

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ANTIOXIDANT ACTIVITY OF FOURTEEN EULOPHIA SPECIES TRADITIONALLY KNOWN AS AMARKAND

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i9.12801 ◽

2016 ◽

Vol 8 (9) ◽

pp. 313

Author(s):

Aarti Narkhede ◽

Minal Mahajan ◽

Elangbam Singh ◽

Abhay Harsulkar ◽

Suresh Jagtap

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Free Radical Scavenging ◽

Antioxidant Potential ◽

Antioxidant Power ◽

Polyphenolic Content ◽

Lipid Peroxidation Assay ◽

Ferric Reducing Antioxidant Power ◽

Reducing Potential

Objective: Amarkand is a group of medicinal plants, of which tubers are regularly consumed by the tribal people in India as food and medicine. It has a strong background of regional ethnopharmacological and food uses. Thus it can be effectively utilized as a source of natural antioxidants. The present study, of fourteen Eulophia species which are predominantly considered as “Amarkand”, were focused for their antioxidant potential and polyphenolic content which facilitates the contribution of the traditional knowledge of plants from India.Methods: Radical scavenging activity, ferric reducing antioxidant power and anti-lipid peroxidation assay were used to evaluate the antioxidant potential. In addition, total phenols, flavonoid and proanthocynidins were estimated.Results: Results showed that all the studied amarkand species have promising antioxidant potential. Eulophia epidendrea (J. Koenig ex. Retz.) C. E. C. Fisch. (EE) and Eulophia mannii (Rchb. f.) Hook. f. (EM) had highest free radical scavenging potential among all. Eulophia epidendrea have highest ferric reducing potential which may be due to high flavonoid and proanthocyanidin content present.Conclusion: The data clearly demonstrated that EE and EM may serve as a good natural antioxidant source against oxidative stress and related diseases.

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Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes

Molecules ◽

10.3390/molecules24050943 ◽

2019 ◽

Vol 24 (5) ◽

pp. 943 ◽

Cited By ~ 1

Author(s):

Wajida Shafi ◽

Sheikh Mansoor ◽

Sumira Jan ◽

Desh Singh ◽

Mohsin Kazi ◽

...

Keyword(s):

Antioxidant Activity ◽

Significant Variation ◽

Hplc Analysis ◽

Phenolic Content ◽

Apple Fruit ◽

Antioxidant Potential ◽

Significant Activity ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Dpph Activity

Catechins and rutin are among the main metabolites found in apple fruit. Sixty apple genotypes, harvested in 2016 and 2017, were analyzed for their phenolic content and antioxidant activity. The HPLC analysis showed that the catechin concentration ranged from 109.98 to 5290.47 µg/g, and the rutin concentration ranged from 12.136 to 483.89 µg/g of apple fruit. The level of DPPH activity ranged from 9.04% to 77.57%, and almost half of the 15 genotypes showed below 30–40% DPPH activity. The apple genotypes ‘Lal Ambri’, ‘Green Sleeves’, and ‘Mallus floribunda’ showed the highest DPPH activity of between 70% and 80%, while ‘Schlomit’, ‘Luxtons Fortune’, ‘Mayaan’, ‘Ananas Retrine’, and ‘Chaubatia ambrose’ showed the lowest ferric reducing antioxidant power (FRAP) activity (0.02–0.09%). Statistical analysis showed a correlation between DPPH activity and catechin content (r = 0.7348) and rutin content (r = 0.1442). Regarding antioxidant activity, fractionated samples of apple genotypes revealed significant activity comparable to that of ascorbic acid. There was also a consistent trend for FRAP activity among all apple genotypes and a significant positive correlation between FRAP activity and rutin content (r = 0.244). Thus, this study reveals a significant variation in antioxidant potential among apple genotypes. This data could be useful for the development of new apple varieties with added phytochemicals by conventional and modern breeders.

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