kappaphycus alvarezii
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2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Evy Yulianti ◽  
Sunarti ◽  
Mae Sri Hartati Wahyuningsih

Abstract Background High glucose concentration increases the glycation process which leads to oxidative stress and inflammation, that can cause complications in diabetes. Several medicinal plants have been used in the treatment of diabetes and its complications. One of them is Kappaphycus alvarezii, an algae that has known antidiabetic abilities. This study aimed to examine the effect of K. alvarezii active fraction on plasma hydrogen peroxide (H2O2) and Tumor Necrosis Factor α (TNFα) levels, renal NADPH oxidase 4 (NOX4) and Nuclear Factor κ B (NFκB) gene expressions. Methods Active fraction was obtained from bioassay-guided fractionation with antiglycation ability. In vivo study was performed on twenty Wistar male rats. The level of H2O2 was measured using H2O2 Assay Kit, the Optical Density value measured using spectrophotometer at a wavelength of 405 nm. Plasma TNFα level was measured using ELISA. Renal NOX4 and NFκB gene expression was analyzed using qPCR. Results Active fraction significantly reduced plasma H2O2 but not TNFα levels. Furthermore, renal NOX4 gene expression was lower in the diabetic rat group treated with active fraction compared to the untreated group but not NFκB gene expression. Conclusions K. alvarezii active fraction has an activity to reduce plasma H2O2 as well as renal NOX4 gene expression. Therefore, this fraction could be developed as a potential candidate for diabetes treatment through oxidative stress mechanisms.


Author(s):  
Ermawaty Maradhy ◽  
Rizal Syarief Nazriel ◽  
Surjono Hadi Sutjahjo ◽  
Meika Syahbana Rusli ◽  
Widiatmaka Widiatmaka ◽  
...  

Tarakan dry seaweed production increased during 2012-2018 for the cultivation of Kappaphycus alvarezii with the longline planting method. This study aims to assess the quality of the waters and their suitability for seaweed cultivation on the coast of Tarakan Island. The environmental parameters of water quality measured were chlorophyll-a, water temperature, salinity, pH, DO, TDS, turbidity, nitrate, phosphate, water depth, current velocity, protection, research location, and distance between settlements. The study was conducted with in-situ and ex-situ measurements based on APHA (2012). Water samples were taken using the multi-parameter Horiba U51. chlorophyll-a was analyzed using spectrophotometric methods, nitrate levels were analyzed using SNI 06-6989.79-2011, while phosphates were analyzed using SNI 06-6989.31-2005. The suitability of the waters is divided into 4 classes, S1 (very suitable), S2 (suitable), S3 (marginally suitable), and N (not suitable). The results showed that the coast of Tarakan Island has 3 categories, namely marginally suitable (S3) 13.20%, suitable (S2) 86.50%, and very suitable (S1) 0.30%. The existing condition of the coastal waters of Tarakan Island supports the cultivation of K. alvarezii seaweed. Tarakan Island coastal existing condition as an areal support seaweed cultivation K. alvarezii with the potential to be developed land area of 33896.73 ha.


2022 ◽  
Vol 6 (6) ◽  
Author(s):  
Asri Silvana Naiu ◽  
Nikmawatisusanti Yusuf ◽  
Yusnina Hudongi

This study aimed to analyze changes in the number of molds and the level of panelist acceptance of K. alvarezii jelly candy packaged using gelatin-CMC edible film during storage. The method used in this study is a laboratory experimental method which consists of two stages, namely preliminary research which aims to determine the best edible film formula to be used as jelly candy packaging and the main research to analyze changes in the number of molds and the level of acceptance of appearance, taste, aroma and texture of jelly candy that has been packaged using a gelatin-CMC edible film during storage. Storage for 60 days was converted to 15 days in a climatic chamber with a temperature of 40 ℃ and a humidity of 75%. Sampling was carried out every 3 days. The research design used linear regression to see the relationship between storage time and test parameters. The results of the preliminary study showed the best formula for edible film, namely 1% gelatin, 0.3% bees wax, 0.75% CMC, and 0.3% glycerol which resulted in a water vapor transmission rate of 18.60 g/m2/24 hours. The results of the main study showed that storage time had a moderate effect on mold. Mold was detected on the last day of observation, storage time had a very strong effect on the level of acceptance which decreased with storage time. The organoleptic value of jelly candy packaged using gelatin-CMC edible film was still acceptable to the panelists until the 36th day of storage.


Aquaculture ◽  
2022 ◽  
Vol 547 ◽  
pp. 737520
Author(s):  
S.U. Mohammed Riyaz ◽  
D. Inbakandan ◽  
P. Bhavadharani ◽  
R. Gopika ◽  
J. Elson ◽  
...  

Data in Brief ◽  
2022 ◽  
pp. 107784
Author(s):  
Rennielyn Rupert ◽  
Kenneth Francis Rodrigues ◽  
Harry Lye Hin Chong ◽  
Wilson Thau Lym Yong

2021 ◽  
Vol 3 (2) ◽  
pp. 113
Author(s):  
Afifah Nurazizatul Hasanah ◽  
Aris Munandar ◽  
Dini Surilayani ◽  
Sakinah Haryati ◽  
Rifki Prayoga Aditia ◽  
...  

2021 ◽  
Vol 5 ◽  
Author(s):  
Khanjan Trivedi ◽  
Vijay Anand K. Gopalakrishnan ◽  
Ranjeet Kumar ◽  
Arup Ghosh

Kappaphycus alvarezii seaweed extract (KSWE) has been known for its plant biostimulant and stress alleviation activities on various crops. However, very few reports are available depicting its impact at the molecular level, which is crucial in identifying the mechanism of action of KSWE on plants. Here, maize leaf tissue of control and KSWE-treated plants were analyzed for their transcriptional changes under drought stress. KSWE was applied foliarly at the V5 stage of maize crop under drought, and leaf transcriptome analysis was performed. It was found that a total of 380 and 631 genes were up- and downregulated, respectively, due to the application of KSWE. Genes involved in nitrate transportation, signal transmission, photosynthesis, transmembrane transport of various ions, glycogen, and starch biosynthetic processes were found upregulated in KSWE-treated plants, while genes involved in the catabolism of polysaccharide molecules such as starch as well as cell wall macromolecules like chitin and protein degradation were found downregulated. An overview of differentially expressed genes involved in metabolic as well as regulatory processes in KSWE-treated plants was also analyzed via Mapman tool. Phytohormone signaling genes such as cytokinin-independent 1 (involved in cytokine signal transduction), Ent-kaurene synthase and GA20 oxidase (involved in gibberellin synthesis), and gene of 2-oxoglutarate-dependent dioxygenase enzyme activity (involved in ethylene synthesis) were found upregulated while 9-cis-epoxycarotenoid dioxygenase (a gene involved in abscisic acid synthesis) was found downregulated due to the application of KSWE. Modulation of gene expression in maize leaf tissue in response to KSWE treatment elucidates mechanisms to ward off drought stress, which can be extended to understand similar phenomenon in other crops as well. This molecular knowledge can be utilized to make the use of KSWE more efficient and sustainable.


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