Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation

In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min.

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Production of gibberellic acid by fed-batch solid state fermentation in an aseptic pilot-scale reactor

Process Biochemistry ◽

10.1016/s0032-9592(96)00063-5 ◽

1997 ◽

Vol 32 (2) ◽

pp. 141-145 ◽

Cited By ~ 38

Author(s):

S. Bandelier ◽

R. Renaud ◽

A. Durand

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Pilot Scale ◽

Fed Batch ◽

Pilot Scale Reactor ◽

Scale Reactor

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Enhanced Cellulase Production in Fed-Batch Solid State Fermentation ofTrichoderma virideSL-1

Journal of Chemical Technology & Biotechnology ◽

10.1002/(sici)1097-4660(199708)69:4<429::aid-jctb732>3.0.co;2-q ◽

1997 ◽

Vol 69 (4) ◽

pp. 429-432 ◽

Cited By ~ 6

Author(s):

Sun Tao ◽

Liu Beihui ◽

Li Zuohu

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Cellulase Production ◽

Fed Batch

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Production of Lignocellulolytic Enzymes and Biomass of Trametes versicolor from Agro-Industrial Residues in a Novel Fixed-Bed Bioreactor with Natural Convection and Forced Aeration at Pilot Scale

Processes ◽

10.3390/pr9020397 ◽

2021 ◽

Vol 9 (2) ◽

pp. 397

Author(s):

Sandra Montoya ◽

Alejandra Patiño ◽

Óscar J. Sánchez

Keyword(s):

Natural Convection ◽

Solid State ◽

Trametes Versicolor ◽

Fixed Bed ◽

Pilot Scale ◽

Lignocellulolytic Enzymes ◽

Fixed Bed Bioreactor ◽

Forced Aeration ◽

First Time ◽

Maximum Biomass Production

Solid-state fermentation requires the development of more efficient cultivation systems for its industrial application. The objective of this work was to evaluate the effect of aeration regime on the production of biomass and several lignocellulolytic enzymes (laccase, manganese peroxidase, endoxylanase, β-glucosidase, and total cellulases) by Trametes versicolor in a novel fixed-bed solid-state pilot-scale bioreactor. Three regimes were assessed: natural convection, low aeration level (10 min every 6 h at 10 L/h air flowrate), and high aeration level (1 h every 6 h at 10 L/h air flowrate). The mushroom was grown on a medium based on lignocellulosic residues. The design of the bioreactor, as well as the control of aeration, humidity, and temperature of the beds, allowed T. versicolor to grow properly on the medium, reaching a maximum biomass production of 204.7 mg/g dry solid (ds). The influence that aeration regime had on the production of lignocellulolytic enzymes was determined. Low level of forced aeration favored obtaining the highest titers of laccase (6.37 U/g ds) compared to natural convection and high aeration level. The greatest lignin degradation was also verified for low aeration regime. For the first time, pilot scale cultivation of T. versicolor was reported in a fixed-bed bioreactor.

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