scholarly journals Production of Lignocellulolytic Enzymes and Biomass of Trametes versicolor from Agro-Industrial Residues in a Novel Fixed-Bed Bioreactor with Natural Convection and Forced Aeration at Pilot Scale

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 397
Author(s):  
Sandra Montoya ◽  
Alejandra Patiño ◽  
Óscar J. Sánchez
Keyword(s):  
Natural Convection ◽  
Solid State ◽  
Trametes Versicolor ◽  
Fixed Bed ◽  
Pilot Scale ◽  
Lignocellulolytic Enzymes ◽  
Fixed Bed Bioreactor ◽  
Forced Aeration ◽  
First Time ◽  
Maximum Biomass Production

Solid-state fermentation requires the development of more efficient cultivation systems for its industrial application. The objective of this work was to evaluate the effect of aeration regime on the production of biomass and several lignocellulolytic enzymes (laccase, manganese peroxidase, endoxylanase, β-glucosidase, and total cellulases) by Trametes versicolor in a novel fixed-bed solid-state pilot-scale bioreactor. Three regimes were assessed: natural convection, low aeration level (10 min every 6 h at 10 L/h air flowrate), and high aeration level (1 h every 6 h at 10 L/h air flowrate). The mushroom was grown on a medium based on lignocellulosic residues. The design of the bioreactor, as well as the control of aeration, humidity, and temperature of the beds, allowed T. versicolor to grow properly on the medium, reaching a maximum biomass production of 204.7 mg/g dry solid (ds). The influence that aeration regime had on the production of lignocellulolytic enzymes was determined. Low level of forced aeration favored obtaining the highest titers of laccase (6.37 U/g ds) compared to natural convection and high aeration level. The greatest lignin degradation was also verified for low aeration regime. For the first time, pilot scale cultivation of T. versicolor was reported in a fixed-bed bioreactor.

Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis

2019 ◽  
Vol 144 ◽  
pp. 1-7 ◽  
Author(s):  
Sabrini N.S. Ávila ◽  
Melissa L.E. Gutarra ◽  
Roberto Fernandez-Lafuente ◽  
Elisa D.C. Cavalcanti ◽  
Denise M.G. Freire
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fixed Bed ◽  
Lipase Production ◽  
Fixed Bed Bioreactor

Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation

2011 ◽  
Vol 58-59 ◽  
pp. 39-49 ◽  
Author(s):  
Viviane Astolfi ◽  
Jaderson Joris ◽  
Ricardo Verlindo ◽  
J. Vladimir Oliveira ◽  
Francisco Maugeri ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fixed Bed ◽  
Fed Batch ◽  
Fixed Bed Bioreactor

scholarly journals Concurrently Produce a Novel High-Potency Sweetener (Glycyrrhetic Acid 3-O-Mono-β-D-glucuronide) and Lignocellulolytic Enzymes using Plant Entophytic Chaetomium Globosum DX-THS3 by Solid-State Fermentation

2021 ◽  
Author(s):  
Boliang Gao ◽  
Yiwen Xiao ◽  
Qian Zhang ◽  
Junru Sun ◽  
Zhibing Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Chaetomium Globosum ◽  
Glycyrrhetic Acid ◽  
Lignocellulolytic Enzymes ◽  
High Potency ◽  
Glucuronidase Activity ◽  
Percent Conversion ◽  
First Time

Abstract Licorice straw was used for the first time as a medium for glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) and lignocellulosic enzyme production via solid-state fermentation (SSF) of endophytic fungus Chaetomium globosum DX-THS3. Under optimal fermentation conditions, the percent conversion of glycyrrhizin reached 90% in 15 days, whereas the control needed 35 days to achieve the same result. The productivity of optimization (P=2.1 mg•g-1•day-1) was 2.33-fold that of non-optimization (P=0.9 mg•g-1•day-1). Meanwhile, high activities of filter paper enzyme (FPase) (234.6 U/g), carboxymethyl cellulase (CMCase) (29.25 U/g), xylanase (72.52 U/g), and β-glucuronidase activity (264.17 U/g) were obtained faster than those in the control during SSF. Our study provides a novel and efficient strategy for GAMG production and indicates C. globosum DX-THS3 as a potential producer of lignocellulolytic enzymes.

scholarly journals Biological manganese removal from mine drainage in a fixed-bed bioreactor at pilot-scale

2019 ◽  
Author(s):  
Jérôme Jacob ◽  
Isabelle Raignault ◽  
Fabienne Battaglia-Brunet ◽  
Carole Mailhan-Muxi ◽  
Jeremy Engevin ◽  
...  
Keyword(s):  
Mine Drainage ◽  
Fixed Bed ◽  
Pilot Scale ◽  
Manganese Removal ◽  
Fixed Bed Bioreactor

Bis(1-phenyl-1-propyne) complexes of tungsten(II): crystal structures of [WI2(CO)(NCR)(η2-MeC2Ph)2] (R = Me, Et, or Ph)

2001 ◽  
Vol 79 (3) ◽  
pp. 263-271
Author(s):  
Paul K Baker ◽  
Michael GB Drew ◽  
Deborah S Evans
Keyword(s):  
Carbon Monoxide ◽  
Solid State ◽  
Crystal Structures ◽  
Octahedral Geometry ◽  
X Ray ◽  
X Ray Crystallography ◽  
Alkyne Complexes ◽  
First Time

Reaction of [WI2(CO)3(NCMe)2] with two equivalents of 1-phenyl-1-propyne (MeC2Ph) in CH2Cl2, and in the absence of light, gave the bis(1-phenyl-1-propyne) complex [WI2(CO)(NCMe)(η2-MeC2Ph)2] (1) in 77% yield. Treatment of equimolar quantities of 1 and NCR (R = Et, i-Pr, t-Bu, Ph) in CH2Cl2 afforded the nitrile-exchanged products, [WI2(CO)(NCR)(η2-MeC2Ph)2] (2-5) (R = Et (2), i-Pr (3), t-Bu (4), Ph (5)). Complexes 1, 2, and 5 were structurally characterized by X-ray crystallography. All three structures have the same pseudo-octahedral geometry, with the equatorial sites being occupied by cis and parallel alkyne groups, which are trans to the cis-iodo groups. The trans carbon monoxide and acetonitrile ligands occupy the axial sites. In structures 1 and 2, the methyl and phenyl substituents of the 1-phenyl-1-propyne ligands are cis to each other, whereas for the bulkier NCPh complex (5), the methyl and phenyl groups are trans to one another. This is the first time that this arrangement has been observed in the solid state in bis(alkyne) complexes of this type.Key words: bis(1-phenyl-1-propyne), carbonyl, nitrile, diiodo, tungsten(II), crystal structures.

Pilot-scale plant study on solid-state metalized reduction–magnetic separation for magnesium-rich nickel oxide ores

2017 ◽  
Vol 169 ◽  
pp. 99-105 ◽  
Author(s):  
Baozhong Ma ◽  
Weijiao Yang ◽  
Peng Xing ◽  
Chengyan Wang ◽  
Yongqiang Chen ◽  
...  
Keyword(s):  
Solid State ◽  
Nickel Oxide ◽  
Magnetic Separation ◽  
Pilot Scale

Effect of salinity on nitrification efficiency and structure of ammonia-oxidizing bacterial communities in a submerged fixed bed bioreactor

2015 ◽  
Vol 266 ◽  
pp. 233-240 ◽  
Author(s):  
C. Cortés-Lorenzo ◽  
M. Rodríguez-Díaz ◽  
D. Sipkema ◽  
B. Juárez-Jiménez ◽  
B. Rodelas ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Fixed Bed ◽  
Fixed Bed Bioreactor

Gold(I) Complexes Bearing P∩N-Ligands: An Unprecedented Twelve-membered Ring Structure Stabilized by Aurophilic Interactions

2009 ◽  
Vol 64 (11-12) ◽  
pp. 1513-1524 ◽  
Author(s):  
Uwe Monkowius ◽  
Manfred Zabel ◽  
Michel Fleck ◽  
Hartmut Yersin
Keyword(s):  
Solid State ◽  
Ring Structure ◽  
Phosphine Ligands ◽  
X Ray Crystallography ◽  
Aggregation Pattern ◽  
Elemental Analyses ◽  
High Yields ◽  
First Time ◽  
Solid State Structures ◽  
Aurophilic Interactions

The P∩N-ligands Ph2Pqn, 1, Ph2 Piqn, 2, Ph2 Ppym, 3, and the As∩N-ligands Ph2Asqn, 4, Ph2Asiqn, 5, (Ph = phenyl, qn = 8-quinoline, iqn = 1-isoquinoline, pym = 2-pyrimidine) have been synthesized, the ligands 2 and 5 for the first time. Their ligand properties were probed by the synthesis of gold(I) complexes. Reaction with (tht)AuCl (tht = tetrahydrothiophene) yielded the chlorogold complexes Ph2RP-Au-Cl (R = qn, 6; iqn, 7; pym, 8) and Ph2RAs-Au-Cl (R = qn, 9; iqn, 10) in high yields. Further treatment of 7 and 8 with one equivalent of AgBF4 provided the complexes [(Ph2Piqn)Au]BF4, 11, [(Ph2Ppym)Au]BF4, 12, and [(Ph2Piqn)Au(tht)]BF4, 14. For comparison, the previously reported complex [(Ph2Ppy)Au]BF4 (py = pyridine), 13, was re-investigated. The compounds were characterized by elemental analyses, mass spectrometry and NMR spectroscopy. In addition, the solid-state structures of 2, 3, 6, 7, 9 - 14 have been determined by X-ray crystallography. The chloro-gold compounds crystallize in the common rod-like structure known from R3EAuCl (R = aryl, E = P, As) complexes without further aggregation via aurophilic interactions. In all cases the phosphine acts as a monodentate ligand. In the solid state compounds 11 - 13 feature an unprecedented cyclic trinuclear aggregation pattern, in which the Au(I) atoms are linearly coordinated by the bridging phosphine ligands forming a cyclic (P-Au-N)3 arrangement. The resulting twelvemembered ring is further stabilized by Au · · · Au interactions. Due to the presence of these Au · · · Au contacts, 11 - 13 are emissive in the solid state but not in solution

scholarly journals Solid-state fermentation of soybean residues for bioflocculant production in a pilot-scale bioreactor system

2014 ◽  
Vol 70 (6) ◽  
pp. 1032-1039 ◽  
Author(s):  
Zufarzaana Zulkeflee ◽  
Antoni Sánchez
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Microbial Growth ◽  
Future Prospect ◽  
Pilot Scale ◽  
Scale Level ◽  
Bioactive Materials ◽  
Current Dependence ◽  
Death Phase ◽  
Bioreactor System

An innovative approach using soybean residues for the production of bioflocculants through solid-state fermentation was carried out in 4.5 L near-to-adiabatic bioreactors at pilot-scale level. An added inoculum of the strain Bacillus subtilis UPMB13 was tested in comparison with control reactors without any inoculation after the thermophilic phase of the fermentation. The flocculating performances of the extracted bioflocculants were tested on kaolin suspensions, and crude bioflocculants were obtained from 20 g of fermented substrate through ethanol precipitation. The production of bioflocculants was observed to be higher during the death phase of microbial growth. The bioflocculants were observed to be granular in nature and consisted of hydroxyl, carboxyl and methoxyl groups that aid in their flocculating performance. The results show the vast potential of the idea of using wastes to produce bioactive materials that can replace the current dependence on chemicals, for future prospect in water treatment applications.

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