Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds

2020 ◽  
Vol 160 ◽  
pp. 107565
Author(s):  
Wei Liu ◽  
Mi Zhang ◽  
Yan Xiao ◽  
Zhaoyang Ye ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Ethylene Terephthalate ◽  
Packed Bed ◽  
Packed Bed Bioreactor ◽  
In Vitro Evaluation ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

Surface Characterization and In Vitro Blood Compatibility of Poly(Ethylene Terephthalate) Immobilized with Hirudin

2005 ◽  
Vol 288-289 ◽  
pp. 421-424
Author(s):  
F. Li ◽  
Jin Wang ◽  
H. Sun ◽  
Nan Huang
Keyword(s):  
Free Energy ◽  
Photoelectron Spectroscopy ◽  
Surface Characterization ◽  
Ethylene Terephthalate ◽  
Blood Compatibility ◽  
Argon Plasma ◽  
Angle Measurement ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

Poly(ethylene terephthalate) films were exposed under argon plasma glow discharge and induced polymerization of acrylic acid (AA) in order to introduce carboxylic acid group onto PET (PET-AA) assisting by ultroviolet radiation. Hirudin-immobilized PETs were prepared by the grafting of PET-AA, followed by chemical reaction with hirudin. The surface structure of the treated PET is determined by X-ray photoelectron spectroscopy (XPS). The wettability and surface free energy, interface free energy of the films is investigated by contact angle measurement. Platelet adhesion evaluatiion is conducted to examine the blood compatibility in vitro. Scanning electron microscopy (SEM) and optical microscopy reveal that the amounts of adhered, aggregated and morphologically changed platelets are reduced on hirudin-immobilized PET films.

In vivo and in vitro responses to poly(ethylene terephthalate-co-diethylene glycol terephthalate) and polyethylene oxide blends

Biomaterials ◽  
1998 ◽  
Vol 19 (22) ◽  
pp. 2075-2082 ◽  
Author(s):  
Ivonete O. Barcellos ◽  
Sonia G. Carobrez ◽  
Alfredo T.N. Pires ◽  
Marcio Alvarez-Silva
Keyword(s):  
Polyethylene Oxide ◽  
Diethylene Glycol ◽  
Ethylene Terephthalate ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing

2010 ◽  
Vol 638-642 ◽  
pp. 612-617
Author(s):  
Jacqueline Marchand-Brynaert
Keyword(s):  
Ethylene Terephthalate ◽  
Serum Free Medium ◽  
Iron Oxide Particles ◽  
Poly Ethylene Terephthalate ◽  
Spacer Arm ◽  
Integrin Antagonists ◽  
Oxide Particles ◽  
Poly Ethylene ◽  
20 Nm

Arg-Gly-Asp (RGD) peptidomimetics constructed on the tyrosine scaffold were equipped with an oligoethylene glycol (OEG) spacer-arm for surface grafting. Their in vitro activities as V3 integrin antagonists were in the range of 0.3-0.7 nM. Poly (ethylene terephthalate) (PET) membranes derivatized with peptidomimetics (50-120 pmol/cm2) allowed the adhesion and proliferation of endothelial cells (HSV) in serum-free medium. Iron oxide particles for MR Imaging grafted with peptidomimetics (2-3 molecules/particle of 20 nm diameter) were selectively targeted to activated tumour cells (Jurkat).

Surface Characterization and Antibacterial Evaluation of Poly(Ethylene Terephthalate) Modified by Chitosan-Immobilization

2005 ◽  
Vol 288-289 ◽  
pp. 331-334 ◽  
Author(s):  
Peng Li ◽  
Jin Wang ◽  
W.C. Lu ◽  
H. Sun ◽  
Nan Huang
Keyword(s):  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Surface Characterization ◽  
Ethylene Terephthalate ◽  
Attenuated Total Reflection ◽  
Poly Ethylene Terephthalate ◽  
Modified Surface ◽  
Poly Ethylene ◽  
Pet Films

Biomedical PET films were modified by the approach of chitosan-surface-grafting. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) revealed that chitosan molecules were successfully grafted on the PET surface. The bacterial adhesion on the modified surface was evaluated by bacteria plate counting in vitro and scanning electron microscopy (SEM). The results testified that chitosan did make the surface of PET become more antibacterial. The free energy of adhesion (∆Fadh) between the bacteria and the chitosan-immobilized surface of PET was calculated. The value of the ∆Fadh was positive, which suggests that the process of bacterial adhesion on the modified PET surface was not thermodynamically favorable, namely, not spontaneous.

Blood Compatibility of Chitosan Immobilized on Poly(Ethylene Terephthalate) Surface Modified by Plasma and Ultraviolet Grafting

2005 ◽  
Vol 288-289 ◽  
pp. 327-330 ◽  
Author(s):  
Jin Wang ◽  
P. Li ◽  
H. Sun ◽  
Ping Yang ◽  
Y.X. Leng ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Ethylene Terephthalate ◽  
Blood Compatibility ◽  
Attenuated Total Reflection ◽  
Grafting Modification ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene ◽  
Surface Modified ◽  
Pet Films

We report a study involving chitosan chains immobilized on poly (ethylene terephthalate) (PET) films by plasma and ultraviolet (UV) grafting modification. The surface structure of the modified PET is determined by means of attenuated total reflection Fourier transform infraed spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results show that the chains of chitosan are successfully grafted on the surface of PET. Platelet adhesion evaluation in vitro is conducted to examine the blood compatibility in vitro. Scanning electron microscopy (SEM) and optical microscopy reveal that the amounts of adhered, aggregated and morphologically changed platelets are reduced on the chitosan-immobilized PET films. The number of platelet adhered on the modified film is reduced by almost 48% compared to the amount of platelets on the untreated film. Our result thus shows that chitosan immobilized on the PET surface improves blood compatibility.

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