scholarly journals Antigenotoxic potential of curcumin and carvacrol against malathion-induced DNA damage in cultured human peripheral blood and its relation to GSTM1 and GSTT1 polymorphism

2015 ◽  
Vol 7 (3) ◽  
pp. 98-104 ◽  
Author(s):  
Neeraj Kumar ◽  
Anita Yadav ◽  
Sachin Gulati ◽  
Kanupriya ◽  
Neeraj Aggarwal ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Gstt1 Polymorphism

scholarly journals HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production

Redox Biology ◽  
2018 ◽  
Vol 15 ◽  
pp. 97-108 ◽  
Author(s):  
Rawan El-Amine ◽  
Diego Germini ◽  
Vlada V. Zakharova ◽  
Tatyana Tsfasman ◽  
Eugene V. Sheval ◽  
...  
Keyword(s):  
Dna Damage ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Ros Production ◽  
Tat Protein ◽  
Mitochondrial Ros ◽  
Hiv 1

scholarly journals Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

2007 ◽  
Vol 40 (4) ◽  
pp. 476-478 ◽  
Author(s):  
Renata Aparecida Martinez Antunes Ribeiro-Vieira ◽  
Daniel Araki Ribeiro ◽  
Daisy Maria Favero Salvadori ◽  
Sílvio Alencar Marques
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Paracoccidioides Brasiliensis ◽  
Peripheral Blood Cells ◽  
Genetic Damage ◽  
Systemic Fungal Infection ◽  
Dna Breakage

Paracoccidioidomycosis is a systemic fungal infection caused by Paracoccidioides brasiliensis. As infectious diseases can cause DNA damage, the authors aimed at analyzing DNA breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. The results suggested that paracoccidioidomycosis does not cause genotoxicity.

scholarly journals Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes ExposedIn Vitroto Neonicotinoid Insecticides News

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
María Elena Calderón-Segura ◽  
Sandra Gómez-Arroyo ◽  
Rafael Villalobos-Pietrini ◽  
Carmen Martínez-Valenzuela ◽  
Yolanda Carbajal-López ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blue Dye ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Neonicotinoid Insecticides ◽  
Human Peripheral Blood Lymphocytes

Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposedin vitroto different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to9.5×10-6to5.7×10-5 M Jade;2.8×10-4to1.7×10-3 M Gaucho;0.6×10-1to1.4×10-1 M Calypso;1.2×10-1to9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to18×10-3 M Jade,2.0×10-3 M Gaucho,2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at30×10-3 M Jade,3.3×10-3 M Gaucho,2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL followingin vitroexposure to commercial neonicotinoid insecticides.

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