Electrochemical DNA biosensor for the detection of DNA hybridization with the amplification of Au nanoparticles and CdS nanoparticles

2009 ◽  
Vol 75 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Ping Du ◽  
Hongxia Li ◽  
Zhenhua Mei ◽  
Shufeng Liu
Keyword(s):  
Dna Hybridization ◽  
Au Nanoparticles ◽  
Dna Biosensor ◽  
Cds Nanoparticles ◽  
Electrochemical Dna Biosensor

scholarly journals Application of Gold Nanoparticles for Electrochemical DNA Biosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ahmed Mishaal Mohammed ◽  
Ruslinda A. Rahim ◽  
Ibraheem Jaleel Ibraheem ◽  
Foo Kai Loong ◽  
Hasrul Hisham ◽  
...  
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Base Line ◽  
Frequency Range ◽  
Dna Immobilization ◽  
Electrochemical Dna Biosensor ◽  
Chemically Modified

An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2thin films. The SiO2thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2thin films using APTES and GNPs were successfully developed for DNA detection.

A novel electrochemical DNA biosensor construction based on layered CuS–graphene composite and Au nanoparticles

2014 ◽  
Vol 406 (27) ◽  
pp. 6943-6951 ◽  
Author(s):  
Chun-Xuan Xu ◽  
Qiu-Ge Zhai ◽  
Yu-Jie Liu ◽  
Ke-Jing Huang ◽  
Lu Lu ◽  
...  
Keyword(s):  
Au Nanoparticles ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Graphene Composite

Development of an electrochemical DNA biosensor based on Quercetin as a new electroactive indicator for DNA hybridization detection

2021 ◽  
Author(s):  
Esmaeel Alipour ◽  
Sheida Norouzi ◽  
Shokoufe Moradi
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Dna Hybridization ◽  
Electrode Surface ◽  
Dna Biosensor ◽  
Carbon Electrode ◽  
Electrochemical Dna Biosensor ◽  
Specific Target ◽  
Target Dna ◽  
Hybridization Detection

Electrochemical DNA biosensor is designed for detection of specific target DNA after hybridization with complementary probe DNA immobilized onto glassy carbon electrode surface. Quercetin was successfully used as a new...

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