scholarly journals In vivo biodistribution studies and ex vivo lymph node imaging using heavy metal-free quantum dots

Biomaterials ◽  
2016 ◽  
Vol 104 ◽  
pp. 182-191 ◽  
Author(s):  
Elnaz Yaghini ◽  
Helen D. Turner ◽  
Alix M. Le Marois ◽  
Klaus Suhling ◽  
Imad Naasani ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Quantum Dots ◽  
Lymph Node ◽  
Ex Vivo ◽  
Metal Free ◽  
Lymph Node Imaging ◽  
In Vivo Biodistribution ◽  
Biodistribution Studies

scholarly journals Functionalizing NaGdF4:Yb,Er Upconverting Nanoparticles with Bone-Targeting Phosphonate Ligands: Imaging and In Vivo Biodistribution

Inorganics ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 60 ◽  
Author(s):  
Silvia Alonso-de Castro ◽  
Emmanuel Ruggiero ◽  
Aitor Lekuona Fernández ◽  
Unai Cossío ◽  
Zuriñe Baz ◽  
...  
Keyword(s):  
Near Infrared ◽  
Ex Vivo ◽  
Mri Contrast Agents ◽  
Infrared Light ◽  
In Vivo Biodistribution ◽  
Bone Targeting ◽  
Biodistribution Studies ◽  
Positron Emission ◽  
Pet Probes

Lanthanide-doped upconverting nanoparticles (UCNPs) transform near infrared light (NIR) into higher-energy UV and visible light by multiphotonic processes. Owing to such unique feature, UCNPs have found application in optical imaging and have been investigated for the NIR light activation of prodrugs, including transition metal complexes of interest in photochemotherapy. Besides, UCNPs also function as magnetic resonance imaging (MRI) contrast agents and positron emission tomography (PET) probes when labelled with radionuclides such as 18F. In this contribution, we report on a new series of phosphonate-functionalized NaGdF4:Yb,Er UCNPs that show affinity for hydroxyapatite (inorganic constituent of bones), and we discuss their potential as bone targeting multimodal (MRI/PET) imaging agents. In vivo biodistribution studies of 18F-labelled NaGdF4:Yb,Er UCNPs in rats indicate that surface functionalization with phosphonates favours the accumulation of nanoparticles in bones over time. PET results reveal leakage of 18F− for phosphonate-functionalized NaGdF4:Yb,Er and control nanomaterials. However, Gd was detected in the femur for phosphonate-capped UCNPs by ex vivo analysis using ICP-MS, corresponding to 6–7% of the injected dose.

Fluorescent Ag–In–S/ZnS Quantum Dots for Tumor Drainage Lymph Node Imaging In Vivo

2021 ◽  
Vol 4 (2) ◽  
pp. 1029-1037
Author(s):  
Xinyuan Sun ◽  
Mengyao Shi ◽  
Chun Zhang ◽  
Jinting Yuan ◽  
Min Yin ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Lymph Node ◽  
Lymph Node Imaging

scholarly journals Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging

2011 ◽  
Vol 369 (1955) ◽  
pp. 4644-4650 ◽  
Author(s):  
Chulhong Kim ◽  
Todd N. Erpelding ◽  
Ladislav Jankovic ◽  
Lihong V. Wang
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Ex Vivo ◽  
Thick Layer ◽  
Chicken Breast ◽  
Breast Cancer Patients ◽  
Non Invasive ◽  
Lymph Node Imaging ◽  
Chicken Tissue

Clinical translation of photoacoustic (PA) imaging can be facilitated by integration with commercial ultrasound (US) scanners to enable dual-modality imaging. An array-based US scanner was modified for hand-held PA imaging. The performance was benchmarked in terms of signal-to-noise ratio (SNR), axial spatial resolution and sensitivity. PA images of a tube, filled with methylene blue (MB; approx. 30 mM) and placed at various depths in chicken tissue, were acquired. A 5 cm penetration depth was achieved with an 18.6 dB SNR using a laser fluence of 3 mJ cm −2 , only one-seventh of the safety limit (20 mJ cm −2 ). An axial resolution of approximately 400 μm was maintained at all imaging depths. The PA sensitivity to MB placed 2.3 cm deep in chicken tissue was less than 100 μM. Further, after intradermal injection of MB (approx. 30 mM), a rat sentinel lymph node was clearly identified in vivo , beneath a 3.8 cm thick layer of chicken breast. The accumulated concentration of MB in the node was estimated to be approximately 7 mM. The noise-equivalent sensitivities (approx. 2 cm depth) were 17 and 85 μM, ex vivo and in vivo , respectively. These results support the use of this PA system for non-invasive mapping and image-guided needle biopsy of sentinel nodes in breast cancer patients.

scholarly journals Effect of ethanol and cocaine on [11C]MPC-6827 uptake in SH-SY5Y cells

2021 ◽  
Author(s):  
Naresh Damuka ◽  
Miranda Orr ◽  
Paul W. Czoty ◽  
Jeffrey L. Weiner ◽  
Thomas J. Martin ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Ex Vivo ◽  
Neuroblastoma Cells ◽  
Proper Function ◽  
Brain Functions ◽  
Biodistribution Studies ◽  
Positron Emission ◽  
Vitro Binding

AbstractMicrotubules (MTs) are structural units in the cytoskeleton. In brain cells they are responsible for axonal transport, information processing, and signaling mechanisms. Proper function of these processes is critical for healthy brain functions. Alcohol and substance use disorders (AUD/SUDs) affects the function and organization of MTs in the brain, making them a potential neuroimaging marker to study the resulting impairment of overall neurobehavioral and cognitive processes. Our lab reported the first brain-penetrant MT-tracking Positron Emission Tomography (PET) ligand [11C]MPC-6827 and demonstrated its in vivo utility in rodents and non-human primates. To further explore the in vivo imaging potential of [11C]MPC-6827, we need to investigate its mechanism of action. Here, we report preliminary in vitro binding results in SH-SY5Y neuroblastoma cells exposed to ethanol (EtOH) or cocaine in combination with multiple agents that alter MT stability. EtOH and cocaine treatments increased MT stability and decreased free tubulin monomers. Our initial cell-binding assay demonstrated that [11C]MPC-6827 may have high affinity to free/unbound tubulin units. Consistent with this mechanism of action, we observed lower [11C]MPC-6827 uptake in SH-SY5Y cells after EtOH and cocaine treatments (e.g., fewer free tubulin units). We are currently performing in vivo PET imaging and ex vivo biodistribution studies in rodent and nonhuman primate models of AUD and SUDs and Alzheimer's disease.

In vitro binding and in vivo biodistribution studies of the neuroprotective peptide humanin using [125I]humanin derivatives

Peptides ◽  
2009 ◽  
Vol 30 (12) ◽  
pp. 2409-2417 ◽  
Author(s):  
Alexandra Evangelou ◽  
Christos Zikos ◽  
Dimitra Benaki ◽  
Maria Pelecanou ◽  
Penelope Bouziotis ◽  
...  
Keyword(s):  
In Vitro Binding ◽  
In Vivo Biodistribution ◽  
Biodistribution Studies ◽  
Vitro Binding
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