Economical lipid production from crude glycerol using Rhodosporidiobolus fluvialis DMKU-RK253 in a two-stage cultivation under non-sterile conditions

2020 ◽  
Vol 138 ◽  
pp. 105597
Author(s):  
Pirapan Polburee ◽  
Savitree Limtong
Keyword(s):  
Crude Glycerol ◽  
Lipid Production ◽  
Two Stage

scholarly journals Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

2020 ◽  
Vol 8 (2) ◽  
pp. 151 ◽  
Author(s):  
Poontawee ◽  
Limtong
Keyword(s):  
Crude Glycerol ◽  
Lipid Production ◽  
Cell Mass ◽  
Batch Cultivation ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Two Stage ◽  
Microbial Lipid Production ◽  
Constant Feeding ◽  
Fed Batch Cultivation

Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

scholarly journals Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

2020 ◽  
Vol 8 (3) ◽  
pp. 453
Author(s):  
Jeerapan Boonyarit ◽  
Pirapan Polburee ◽  
Bongkot Khaenda ◽  
Zongbao K. Zhao ◽  
Savitree Limtong
Keyword(s):  
Crude Glycerol ◽  
Lipid Production ◽  
Cell Mass ◽  
Production Optimization ◽  
Biodiesel Production ◽  
Glycerol Solution ◽  
High Cell ◽  
Two Stage ◽  
Second Stage ◽  
High Lipid

Lipids from oleaginous microorganisms, including oleaginous yeasts, are recognized as feedstock for biodiesel production. A production process development of these organisms is necessary to bring lipid feedstock production up to the industrial scale. This study aimed to enhance lipid production of low-cost substrates, namely sugarcane top and biodiesel-derived crude glycerol, by using a two-stage cultivation process with Rhodosporidiobolus fluvialis DMKU-SP314. In the first stage, sugarcane top hydrolysate was used for cell propagation, and in the second stage, cells were suspended in a crude glycerol solution for lipid production. Optimization for high cell mass production in the first stage, and for high lipid production in the second stage, were performed separately using a one-factor-at-a-time methodology together with response surface methodology. Under optimum conditions in the first stage (sugarcane top hydrolysate broth containing; 43.18 g/L total reducing sugars, 2.58 g/L soy bean powder, 0.94 g/L (NH4)2SO4, 0.39 g/L KH2PO4 and 2.5 g/L MgSO4 7H2O, pH 6, 200 rpm, 28 °C and 48 h) and second stage (81.54 g/L crude glycerol, pH 5, 180 rpm, 27 °C and 196 h), a high lipid concentration of 15.85 g/L, a high cell mass of 21.07 g/L and a high lipid content of 73.04% dry cell mass were obtained.

Biophotolysis-based hydrogen and lipid production by oleaginous microalgae using crude glycerol as exogenous carbon source

2017 ◽  
Vol 42 (4) ◽  
pp. 1970-1976 ◽  
Author(s):  
Dennapa Sengmee ◽  
Benjamas Cheirsilp ◽  
Thanwadee Tachapattaweawrakul Suksaroge ◽  
Poonsuk Prasertsan
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Lipid Production ◽  
Oleaginous Microalgae ◽  
Exogenous Carbon Source

scholarly journals Economical lipid production from Trichosporon oleaginosus via dissolved oxygen adjustment and crude glycerol addition

2019 ◽  
Vol 273 ◽  
pp. 288-296 ◽  
Author(s):  
Xiaolei Zhang ◽  
Jiaxin Chen ◽  
Di Wu ◽  
Ji Li ◽  
Rajeshwar Dayal Tyagi ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Crude Glycerol ◽  
Lipid Production

scholarly journals Exogenous l-proline improved Rhodosporidium toruloides lipid production on crude glycerol

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Rasool Kamal ◽  
Yuxue Liu ◽  
Qiang Li ◽  
Qitian Huang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Lipid Content ◽  
Crude Glycerol ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biodiesel Production ◽  
Lipid Yield ◽  
Stress Agent ◽  
Glycerol Consumption

Abstract Background Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l-proline as a safeguard. Herein, l-proline was assessed as an anti-stress agent in crude glycerol media. Results Crude glycerol was converted to microbial lipids by the oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 in a two-staged culture mode. The media was supplied with exogenous l-proline to improve lipid production efficiency in high crude glycerol stress. An optimal amount of 0.5 g/L l-proline increased lipid titer and lipid yield by 34% and 28%, respectively. The lipid titer of 12.2 g/L and lipid content of 64.5% with a highest lipid yield of 0.26 g/g were achieved with l-proline addition, which were far higher than those of the control, i.e., lipid titer of 9.1 g/L, lipid content of 58% and lipid yield of 0.21 g/g. Similarly, l-proline also improved cell growth and glycerol consumption. Moreover, fatty acid compositional profiles of the lipid products was found suitable as a potential feedstock for biodiesel production. Conclusion Our study suggested that exogenous l-proline improved cell growth and lipid production on crude glycerol by R. toruloides. The fact that higher lipid yield as well as glycerol consumption indicated that l-proline might act as a potential anti-stress agent for the oleaginous yeast strain.

Co-fermentation of a sugar mixture for microbial lipid production in a two-stage fermentation mode under non-sterile conditions

2020 ◽  
Vol 4 (5) ◽  
pp. 2380-2385
Author(s):  
Wujun Liu ◽  
Weifeng Mao ◽  
Cuili Zhang ◽  
Xi Lu ◽  
Xujie Xiao ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Two Stage ◽  
Sugar Mixture ◽  
Microbial Lipid Production

The economical production of biodiesel from lignocellulose requires an effective co-fermentation of lignocellulose-derived sugars, such as glucose and xylose.

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