Protective effects of a blueberry extract in acute inflammation and collagen-induced arthritis in the rat

AbstractPotential protective effects of elderberry (Sambucus nigra) extracts against oxidative degradation of hyaluronan (HA) were detected in vitro. To induce free-radical-mediated HA degradation, Weissberger’s biogenic oxidative system, which mimics the situation of acute inflammation, was applied. Time- and dose-dependent changes of dynamic viscosity of the HA solutions in the presence and absence of two elderberry extracts produced in 2006 and 2012 were recorded by rotational viscometry (RV). Radical scavenging capacity of both extracts was investigated by the spectrocolorimetric ABTS [2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] assay and the “inverted” ABTS assay. Oxygen consumption of the system oxidizing HA either in the absence or presence of the elderberry extracts was determined. The results of RV revealed that an addition of the newer extract (2012) promoted the inhibition of HA degradation more markedly compared to the older extract (2006). The same effect of both extracts on ABTS

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Gut microbiota mediated the protective effects of tuna oil on collagen-induced arthritis in mice

Food & Function ◽

10.1039/d1fo00709b ◽

2021 ◽

Author(s):

Chenyang Lu ◽

Ju Chen ◽

Congmin Yi ◽

Jiaojiao Han ◽

Qiuyue Shi ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Autoimmune Disease ◽

Gut Microbiota ◽

Protective Effects ◽

Collagen Induced Arthritis ◽

Tuna Oil ◽

Beneficial Effects ◽

Chronic Autoimmune Disease

Rheumatoid arthritis is emerging as a chronic autoimmune disease worldwide. In this study, the components of tuna oil (TO) were firstly measured, and the beneficial effects of TO on collagen-induced...

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Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway

International Journal of Cell Biology ◽

10.1155/2020/8319516 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Fangfang Bi ◽

Wenbo Liu ◽

Zongtao Wu ◽

Chen Ji ◽

Cuicui Chang

Keyword(s):

Intervertebral Disc ◽

Disc Degeneration ◽

Intervertebral Disc Degeneration ◽

Acute Inflammation ◽

Protective Effects ◽

Toll Like Receptor ◽

Inhibitory Effects ◽

Toll Like Receptor 4 ◽

Disc Injury ◽

Anti Inflammation

Antiaging protein Klotho exhibits impressive properties of anti-inflammation, however is declined early after intervertebral disc injury, making Klotho restoration an attractive strategy of treating intervertebral disc inflammatory disorders. Here, we have found that Klotho is enriched in nucleus pulposus (NP) cells and Klotho overexpression attenuates H2O2-induced acute inflammation essentially via suppressing Toll-like receptor 4 (TLR4). The proinflammatory NF-κB signaling and cytokine expressions paralleled with Klotho repression and TLR4 elevation in both NP cells (H2O2 treatment) and rat intervertebral disc (needle puncture treatment). Overexpression of TLR4 downregulated expression of Klotho, whereas interfering TLR4 expression diminished the inhibitory effects of H2O2 on Klotho in NP cells. Consistently, Klotho knockdown by RNA interferences largely diminished the anti-inflammatory and intervertebral disc protective effects in an Intervertebral Disc Degeneration (IDD) model. Thus, our study indicates that TLR4-NF-κB signaling and Klotho form a negative-feedback loop in NP cells. Also, we demonstrate that the expression of Klotho is regulated by the balance between upregulation and downregulation of TLR4-NF-κB signaling.

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