Ardisia crispa root hexane fraction suppressed angiogenesis in human umbilical vein endothelial cells (HUVECs) and in vivo zebrafish embryo model

We previously reported that 3T3-L1 and rat primary adipocytes secreted microvesicles, known as adipocyte-derived microvesicles (ADMs). In the present study, we further characterized the 3T3-L1 ADMs and found that they exhibited angiogenic activity in vivo. Antibody arrays and gelatin zymography analyses revealed that several angiogenic and antiangiogenic proteins, including leptin, TNFα, acidic fibroblast growth factor (FGFa), interferon-γ, and matrix metalloprotease (MMP)-2 and MMP-9, were present in the ADMs. Gene expression of most of these angiogenic factors was induced in the adipose tissue of diet-induced obese mice. Furthermore, leptin, TNFα, and MMP-2 were up-regulated at the protein level in the adipocyte fractions prepared from epididymal adipose tissues of high-fat-diet-induced obese mice. ADMs induced cell migration and tube formation of human umbilical vein endothelial cells, which were partially suppressed by neutralizing antibodies to leptin, TNFα, or FGFa but not to interferon-γ. Supporting these data, a mixture of leptin, TNFα, and FGFa induced tube formation. ADMs also promoted cell invasion of human umbilical vein endothelial cells through Matrigel, which was suppressed by the addition of the MMP inhibitor 1,10′-phenanthroline and a neutralizing antibody to MMP-2 but not to MMP-9. These results suggest that ADMs are associated with multiple angiogenic factors and play a role in angiogenesis in adipose tissue.

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Evaluation of Solid Free-Form Fabrication-Based Scaffolds Seeded with Osteoblasts and Human Umbilical Vein Endothelial Cells for Use In Vivo Osteogenesis

Tissue Engineering Part A ◽

10.1089/ten.tea.2009.0644 ◽

2010 ◽

Vol 16 (7) ◽

pp. 2229-2236 ◽

Cited By ~ 46

Author(s):

Jong Young Kim ◽

Guang-Zhen Jin ◽

In Su Park ◽

Jeong-Nam Kim ◽

So Young Chun ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Free Form ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells ◽

Solid Free Form Fabrication

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Constructing heparin-modified pancreatic decellularized scaffold to improve its re-endothelialization

Journal of Biomaterials Applications ◽

10.1177/0885328217752859 ◽

2018 ◽

Vol 32 (8) ◽

pp. 1063-1070 ◽

Cited By ~ 4

Author(s):

Liancheng Xu ◽

Yibing Guo ◽

Yan Huang ◽

Yicheng Xiong ◽

Yang Xu ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Therapeutic Option ◽

Blue Staining ◽

Potential Candidate ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells ◽

Decellularized Scaffold

Pancreas transplantation is considered as a promising therapeutic option with the potential to cure diabetes. However, efficacy of current clinical transplantation is limited by the donor organ. With regard to creating a functional pancreas-tissue equivalent for transplantation, vascularization remains a large obstacle. To enhance the angiogenic properties of pancreatic decellularized scaffold, surface modification of the vasculature was used to promote endothelialization efficiency. In this study, an endothelialized pancreatic decellularized scaffold was obtained through heparin modification under mild conditions. The immobilization of heparin was performed through 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide and N-Hydroxysuccinimide. The morphology, ultra-structure and porosity of the heparinized scaffold were characterized by toluidine blue staining, scanning electron microscope and infrared spectrum. The adhesion, proliferation and angiogenesis of human umbilical vein endothelial cells on heparin-pancreatic decellularized scaffold were also researched in vitro. In vivo transplantation was also performed to observe the location of human umbilical vein endothelial cells and the formation of new blood vessel, which exhibited significant differences with pancreatic decellularized scaffold group (p<0.05). These findings indicated that the endothelialized heparin-pancreatic decellularized scaffold may be used to solve the problem of blood supply and to support the function of insulin-secreting cells better after in vivo transplantation, and therefore, would be a potential candidate for pancreatic tissue engineering.

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Studies on the acute release of tissue-type plasminogen activator from human endothelial cells in vitro and in rats in vivo: evidence for a dynamic storage pool

Blood ◽

10.1182/blood.v85.12.3510.bloodjournal85123510 ◽

1995 ◽

Vol 85 (12) ◽

pp. 3510-3517 ◽

Cited By ~ 98

Author(s):

Y van den Eijnden-Schrauwen ◽

T Kooistra ◽

RE de Vries ◽

JJ Emeis

Keyword(s):

Endothelial Cells ◽

Plasminogen Activator ◽

Umbilical Vein ◽

Tissue Type ◽

Enzyme Linked Immunosorbent Assay ◽

Tissue Type Plasminogen Activator ◽

Human Umbilical Vein ◽

Type Plasminogen Activator ◽

Umbilical Vein Endothelial Cells

The process of acute release of tissue-type plasminogen activator (tPA) is important in locally speeding up fibrinolysis. Using a sensitive enzyme-linked immunosorbent assay for tPA, we investigated the acute release of tPA from cultured human umbilical vein endothelial cells. The addition of thrombin (0.003 to 3 NIH U/mL) caused the dose-dependent release of noncomplexed, enzymatically active tPA into the medium. The amount of tPA released into the medium by thrombin was similar to the difference in the amounts of tPA present in extracts from thrombin-treated cells and control cells. The process of acute release of tPA was complete in 1 minute, whereas the concomitant release of von Willebrand factor into the medium was slightly slower (maximum after 3 minutes). By increasing (c.q. decreasing) tPA synthesis, it was found that the amount of tPA constitutively secreted, the amount acutely released, and the amount in cell extracts were increased (c.q. decreased) to the same extent. The same relation was found in vivo. When rats were pretreated with cholera toxin or retinoic acid to increase tPA synthesis, plasma levels of tPA were increased, whereas acute release of tPA, as induced by bradykinin, was increased to the same extent. Acutely released tPA and constitutively secreted tPA were liberated from different pathways in human umbilical vein endothelial cells; tPA had, relative to the in vivo situation, a short residence time in the acutely releasable pathway.

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