Characterization of fatty amides produced by lipase-catalyzed amidation of multi-hydroxylated fatty acids

2008 ◽  
Vol 99 (7) ◽  
pp. 2706-2709 ◽  
Author(s):  
William E. Levinson ◽  
Tsung Min Kuo ◽  
Gerhard Knothe
Keyword(s):  
Fatty Acids ◽  
Fatty Amides ◽  
Hydroxylated Fatty Acids

Characterization of Aeromonas genomic species by using quinone, polyamine, and fatty acid patterns

1994 ◽  
Vol 40 (10) ◽  
pp. 844-850 ◽  
Author(s):  
Peter Kämpfer ◽  
Klaus Blasczyk ◽  
Georg Auling
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Octadecenoic Acid ◽  
Hexadecenoic Acid ◽  
Genomic Species ◽  
Great Homogeneity ◽  
Hydroxylated Fatty Acids ◽  
Fatty Acid Patterns ◽  
Chemotaxonomic Study

A chemotaxonomic study was carried out on representative strains of 13 Aeromonas genomic species. Quinone, polyamine, and fatty acid patterns were found to be very useful for an improved characterization of the genus and an improved differentiation from members of the families Enterobacteriaceae and Vibrionaceae. The Q-8-benzoquinone was the predominant ubiquinone, and putrescine and diaminopropane were the major poly amines of the genus. The fatty acid patterns of 181 strains, all characterized by DNA–DNA hybridization, showed a great homogeneity within the genus, with major amounts of hexadecanoic acid (16:0), hexadecenoic acid (16:1), and octadecenoic acid (18:1), and minor amounts of the hydroxylated fatty acids (3-OH 13:0, 2-OH 14:0, 3-OH 14:0) in addition to some iso and anteiso branched fatty acids (i-13:0, i-17:1, i-17:0, and a-17:0). Although some differences in fatty acid profiles between the genomic species could be observed, a clearcut differentiation of all species was not possible.Key words: Aeromonas, polyamines, quinones, fatty acids, differentiation.

Isolation and characterization of nocardia-like variants of Mycobacterium smegmatis

1976 ◽  
Vol 22 (10) ◽  
pp. 1480-1491 ◽  
Author(s):  
R. J. Hawley ◽  
T. Imaeda ◽  
Nora Mann
Keyword(s):  
Fatty Acids ◽  
Mitomycin C ◽  
Ultraviolet Light ◽  
Mycobacterium Smegmatis ◽  
Deoxyribonucleic Acid ◽  
New Class ◽  
Parental Type ◽  
Isolation And Characterization ◽  
Hydroxylated Fatty Acids

Orange-red-pigmented (OR) colonies were isolated from cream-yellow-pigmented Mycobacterium smegmatis after exposure to either mycobacteriophage MC4 or ultraviolet light; these variant strains were designated OR4 and ORuv, respectively. Early subculture of OR-colonies did not show any segregation of parental-type cells. However, colonies resembling the parental strains, possibly representing a back mutant (REV-OR4), were occasionally found during subculture of established OR-colonies or upon treatment with N-nitroso-N′-nitro-N-methylguanidine. The OR-variants were characterized by their lytic response to nocardiophage, but not to mycobacteriophages, presence of α-branched, β-hydroxylated fatty acids of the Nocardia-type, and a guanine plus cytosine value of deoxyribonucleic acid (DNA) between 62 and 64 mol %. They were more resistant to the lethal action of both ultraviolet light and mitomycin C treatment than the parental and back mutant strains.Although the OR-variants in this study possess characteristics common to the genus Nocardia or some of the 'rhodochrous' mycobacteria, evidence is presented that they form a new class of mycobacterial variants.

scholarly journals Characterization of the Solution Structure of Human Serum Albumin Loaded with a Metal Porphyrin and Fatty Acids

2011 ◽  
Vol 100 (9) ◽  
pp. 2293-2301 ◽  
Author(s):  
Matthias J.N. Junk ◽  
Hans W. Spiess ◽  
Dariush Hinderberger
Keyword(s):  
Fatty Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Solution Structure ◽  
Metal Porphyrin

The synthesis and molecular dynamics of phospholipids having hydroxylated fatty acids at the sn-2 position

1990 ◽  
Vol 52 (3-4) ◽  
pp. 217-226 ◽  
Author(s):  
Yisrael Isaacson ◽  
Caroline D. Sherbourne ◽  
Richard W. Gross ◽  
William F. Stenson
Keyword(s):  
Molecular Dynamics ◽  
Fatty Acids ◽  
Hydroxylated Fatty Acids

scholarly journals Channeling of Eukaryotic Diacylglycerol into the Biosynthesis of Plastidial Phosphatidylglycerol

2006 ◽  
Vol 282 (7) ◽  
pp. 4613-4625 ◽  
Author(s):  
Markus Fritz ◽  
Heiko Lokstein ◽  
Dieter Hackenberg ◽  
Ruth Welti ◽  
Mary Roth ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Double Bond ◽  
Transgenic Plants ◽  
Phosphatidic Acid ◽  
Lipid Analysis ◽  
Photosynthetic Apparatus ◽  
Hexadecenoic Acid ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Plastidial glycolipids contain diacylglycerol (DAG) moieties, which are either synthesized in the plastids (prokaryotic lipids) or originate in the extraplastidial compartment (eukaryotic lipids) necessitating their transfer into plastids. In contrast, the only phospholipid in plastids, phosphatidylglycerol (PG), contains exclusively prokaryotic DAG backbones. PG contributes in several ways to the functions of chloroplasts, but it is not known to what extent its prokaryotic nature is required to fulfill these tasks. As a first step toward answering this question, we produced transgenic tobacco plants that contain eukaryotic PG in thylakoids. This was achieved by targeting a bacterial DAG kinase into chloroplasts in which the heterologous enzyme was also incorporated into the envelope fraction. From lipid analysis we conclude that the DAG kinase phosphorylated eukaryotic DAG forming phosphatidic acid, which was converted into PG. This resulted in PG with 2–3 times more eukaryotic than prokaryotic DAG backbones. In the newly formed PG the unique Δ3-trans-double bond, normally confined to 3-trans-hexadecenoic acid, was also found in sn-2-bound cis-unsaturated C18 fatty acids. In addition, a lipidomics technique allowed the characterization of phosphatidic acid, which is assumed to be derived from eukaryotic DAG precursors in the chloroplasts of the transgenic plants. The differences in lipid composition had only minor effects on measured functions of the photosynthetic apparatus, whereas the most obvious phenotype was a significant reduction in growth.

ChemInform Abstract: Derivatization of Keto Fatty Acids. Part 9. Synthesis and Characterization of Oxathiolanes.

ChemInform ◽  
1987 ◽  
Vol 18 (11) ◽  
Author(s):  
S. AHMAD ◽  
M. KHAN ◽  
F. AHMAD ◽  
NASIRULLA NASIRULLA ◽  
S. M. OSMAN
Keyword(s):  
Fatty Acids ◽  
Synthesis And Characterization

scholarly journals Characterization of planktonic and biofilm fatty acid profiles and evaluation of their trophic transfer potential to Hyalella Azteca

2021 ◽  
Author(s):  
Jerry Chien-Yao Chao
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Bacterial Species ◽  
Saturated Fatty Acids ◽  
Hyalella Azteca ◽  
Fatty Acid Profiles ◽  
Fa Composition ◽  
Branched Chain ◽  
Transfer Potential

Fatty acid (FA) composition between biofilms and batch planktonic cultures were compared for two bacterial species Pseudomonas aeruginosa and Staphylococcus aureaus. Biofilm cultures exhibited decrease in saturated fatty acids (SAFA) that potentially conform to a more fluidic biophysical membrane property. The amount of FA in the biofilms' extracellular polymeric substance was not sufficient to consider it having a major contribution to the observed differences between biofilms and batch planktonic cultures. While biofilm grazing by the amphipod Hyalella azteca was evident, only certain bacteria-specific FA appeared to have the potential to be retained (odd-number SAFA and branched-chain FA). H. azteca with diet strictly consisted of bacteria biofilms did not demonstrate significant changes in their nutritional condition in terms of ω-3 and ω-6 polyunsaturated fatty acids (PUFA): combined with the results from fasting trials, H. azteca appears to have the capacity to retain ω-3 and ω-6 PUFAs up to 10 days.

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