Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process

2011 ◽  
Vol 102 (2) ◽  
pp. 2105-2108 ◽  
Author(s):  
Jong Ho Lee ◽  
Sung Bong Kim ◽  
Seong Woo Kang ◽  
Yoon Seok Song ◽  
Chulhwan Park ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Rhizopus Oryzae ◽  
Candida Rugosa ◽  
Biodiesel Production ◽  
Supercritical Carbon

scholarly journals Microwave Irradiation Assisted Supercritical Carbon Dioxide Extraction of Bio-Oils from Chlorella Vulgaris Microalgae

2019 ◽  
Vol 7 (4.14) ◽  
pp. 221
Author(s):  
S. N. Ibrahim ◽  
K. A. Radzun ◽  
K. Ismail
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Supercritical Carbon Dioxide ◽  
Optimum Condition ◽  
Microwave Irradiation ◽  
Chlorella Vulgaris ◽  
Saturated Fatty Acids ◽  
Biodiesel Production ◽  
High Yield ◽  
Supercritical Carbon

Chlorella vulgaris is one of the promising microalgae strains that can produce high yield of bio-oils. The C. vulgaris was pretreated with microwave irradiation prior to extraction using supercritical carbon dioxide (SCCO2). Fourier transform infrared spectroscopy (FTIR) analysis showed microwave irradiation pretreatment does not affect the material composition of C. vulgaris. Scanning electron microscopy (SEM) of the microwave irradiation pretreated microalgae showed an agglomeration of the cells with the cells shape became distorted due to rupturing of the cell walls. Optimization of the SCCO2 process parameters (pressure, temperature and CO2 flow rate) was performed by using response surface methodology (RSM) with central composite design (CCD). Two factors significantly affecting the extraction yield were temperature and pressure. The model equation also predicted the optimum condition for the SCCO2 (without microwave pretreatment) at 70 , 5676 psi and 7 sL/ min while optimum condition for SCCO2 (microwave irradiation pretreatment) at 63 , 5948 psi and 10 sL/ min. High amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), -linolenic acid and palmitoleic acid were found in the extracted oil with microwave irradiation pre-treatment sample.  In addition, the polyunsaturated fatty acids (PUFA) content in the microwave irradiation pretreated oil was considerably low and is desirable for biodiesel production. 

Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide

2011 ◽  
Vol 156 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Kishor P. Dhake ◽  
Krishna M. Deshmukh ◽  
Yogesh P. Patil ◽  
Rekha S. Singhal ◽  
Bhalchandra M. Bhanage
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Rhizopus Oryzae ◽  
Ester Synthesis ◽  
Rhizopus Oryzae Lipase ◽  
Supercritical Carbon

Utilization of Supercritical Carbon Dioxide and Co-solvent n-hexane to Optimize Oil Extraction from Gliricidia sepium Seeds for Biodiesel Production

2021 ◽  
Author(s):  
Maria Cristina Macawile ◽  
Joseph Auresenia
Keyword(s):  
Carbon Dioxide ◽  
Response Surface Methodology ◽  
Supercritical Carbon Dioxide ◽  
Response Surface ◽  
Flow Rate ◽  
Gliricidia Sepium ◽  
Biodiesel Production ◽  
Oil Yield ◽  
Co2 Flow ◽  
Supercritical Carbon

This study was conducted to optimize the supercritical carbon dioxide (scCO2) extraction of oil from Gliricidia sepium seeds using response surface methodology. Initial experiments were carried out using scCO2 and scCO2 with co-solvent n-hexane to determine the effect of co-solvent addition in oil yield. In order to obtain the maximum yield, experiments were conducted using Response Surface Methodology - Face Centered Central Composite Design (RSM – FCCD) under the following conditions: pressure of 20, 30, and 40 MPa, temperature of 50, 60, and 70°C, and CO2 flow rate of 2, 2.5, and 3 mL/min. A second-order polynomial with extended cubic interaction model was significantly fitted (p < 0.05), and a high coefficient determination value (R2 = 0.98) was recorded. At a constant extraction time of 60 minutes, the best extraction yield (12.12%) was obtained at 60°C, 40 MPa, and 2.5 mL/min. The pressure, temperature, and CO2 flow rate were all found to have a significant effect on the oil yield. The oil was used in biodiesel production and its methyl ester composition was analyzed using Gas Chromatography-Flame Ionization Detector (GC-FID).

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