Production of leucine amino peptidase in lab scale bioreactors using Streptomyces gedanensis

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon suspension cultures derived from a single seedling. The enzymes studied included malate, glutamate, and glucose-6-phosphate dehydrogenases; peroxidase; polyphenol oxidase; esterase; acid phosphatase; and leucine amino peptidase. Peroxidase isoenzymes were also detected in the media. The isoenzymatic patterns of the three cultures were different for some enzymes, similar for others, and identical for the rest. The isoenzymatic patterns were recorded on a number of occasions over a period of 3 years and they remained relatively unchanged.

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Leucine amino peptidase a better indicator of disseminated malignant disease

Indian Journal of Clinical Biochemistry ◽

10.1007/bf02894275 ◽

2004 ◽

Vol 19 (2) ◽

pp. 149-151

Author(s):

M. S. Ghadge ◽

Raste

Keyword(s):

Malignant Disease ◽

Leucine Amino Peptidase

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Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic changes during the callus culture cycle and differences between stock cultures

Canadian Journal of Botany ◽

10.1139/b74-341 ◽

1974 ◽

Vol 52 (12) ◽

pp. 2621-2629 ◽

Cited By ~ 10

Author(s):

Paul G. Arnison ◽

W. G. Boll

Keyword(s):

Phaseolus Vulgaris ◽

Polyphenol Oxidase ◽

Callus Culture ◽

Cell Cultures ◽

Callus Cultures ◽

Bush Bean ◽

Leucine Amino Peptidase ◽

Culture Cycle ◽

Enzyme Patterns ◽

Isoenzyme Patterns

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon callus cultures derived from a single seedling. The enzymes studied included peroxidase, polyphenol oxidase, catalase, malate and glutamate dehydrogenases, esterase, and leucine amino peptidase. Enzyme patterns changed during the culture cycle and several isoenzymes appeared only at certain times. The isoenzymatic patterns of the three cultures were very similar but persistent differences between them were observed.

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Allomones confer resistance to Musa cultivar Pisanglilin against infestation by Odoiporus longicollis [Oliver] and characterization of Allomones

Allelopathy Journal ◽

10.26651/allelo.j/2021-53-1-1326 ◽

2021 ◽

Vol 53 (1) ◽

pp. 35-52

Author(s):

K.J Kavitha ◽

L. Sibina L ◽

D.A. Evans

Keyword(s):

Larvicidal Activity ◽

Column Chromatography ◽

Simultaneous Action ◽

Sulfoquinovosyl Diacylglycerol ◽

Leucine Amino Peptidase ◽

Total Free Amino Acids ◽

Preliminary Study ◽

Cuticle Development ◽

Moulting Hormone

In bioassay guided extraction of pseudostem powder of Pisanglilin by organic solvents we found the larvicidal activity in acetone extract, whose column chromatography by methanol-chloroform mixture separated the extract into 9-fractions, of which the 8th fraction showed larvicidal activity. Subfractionation of the active fraction by column chromatography resulted in the isolation of two larvicidal molecules [Stigmasterol-3-O-glucoside (SOG) and Sulfoquinovosyl diacylglycerol (SQDG)]. Yield of SOG was 0.002 % and SQDG was 0.005 % and both were highly toxic to O. longicollis larvae with LD50 of 0.40 and 0.378 ppm, respectively. Larvae fed these compounds stopped feeding on third day and died within one week. SOG inhibited the amylase and protease activity of gut and induced histolysis in the mid gut. While SQDG inhibited the leucine amino peptidase and trypsin like serine protease activities, which decreased the content of total free amino acids. Imbalance in the activities of aspartate amino transferase and alanine amino transferase disrupted the aminoacid metabolism and the compound inhibited the activity of tyrosinase (an enzyme involved in cuticle development). SQDG toxicity caused accumulation of 20-hydroxyecdysone, the active moulting hormone in the hemolymph. Simultaneous action of two allomones present in Pisanglilin effectively resisted the attack of endophytic larvae in the pseudostem and thereby conferred resistance against infestation by O.longicollis. Preliminary study by intrapseudostem injection of Pisanglilin extract in susceptible M.paradisiaca cultivar Kappa, gave complete protection to it from attack by this pest, under field condition.

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An analysis of selection at two loci in fluctuating populations of Microtus

Canadian Journal of Zoology ◽

10.1139/z74-187 ◽

1974 ◽

Vol 52 (12) ◽

pp. 1457-1462 ◽

Cited By ~ 3

Author(s):

D. Alan Birdsall

Keyword(s):

Microtus Pennsylvanicus ◽

Early Mortality ◽

Leucine Amino Peptidase ◽

Fluctuating Populations

Indirect estimates of differential early mortality at the transferrin (Tf) and leucine amino-peptidase (LAP) loci in fluctuating populations of Microtus pennsylvanicus have been obtained. Among individuals first captured during decline phases, there were consistent excesses of males heterozygous at the Tf locus. Among individuals first captured during increase phases, there were consistent excesses of males heterozygous at the LAP locus. It is concluded that these observations are attributable to selection.

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ALLOZYME INHERITANCE AND DIVERSITY IN SOUR CHERRY

HortScience ◽

10.21273/hortsci.25.9.1081a ◽

1990 ◽

Vol 25 (9) ◽

pp. 1081a-1081

Author(s):

James Beaver ◽

Amy Iezzoni

Keyword(s):

Germplasm Collection ◽

Sour Cherry ◽

Starch Gel Electrophoresis ◽

Shikimate Dehydrogenase ◽

Heterozygous Condition ◽

Segregation Data ◽

Disomic Inheritance ◽

Leucine Amino Peptidase ◽

Starch Gel ◽

Allozyme Loci

Starch gel electrophoresis was employed to study inheritance and diversity of allozyme loci in a sour cherry (2n=4×=32) germplasm collection. Segregation data was collected for alcohol dehydrogenase (ADH), glucose phosphate isomerase (GPI), isocitrate dehydrogenase (IDH), leucine amino peptidase (LAP), malate dehydrogenase (MDH), peroxidase (PX) (cathodal activity), phosphoglucomutase (PGM), 6-phosphogluconic dehydrogenase (6-PGD), and shikimate dehydrogenase (s k d h).Data suggest that alleles can be assigned to many of the enzyme systems being studied: ADH, GPI, IDH, LAP, PGM, and 6-PGD. Most loci are diallelic and often exhibit the unbalanced heterozygous condition. MDH, PX, and 6-PGD are highly polymorphic. Progeny segregation data fit disomic inheritance models, indicating that sour cherry is an allotetraploid.

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