Allomones confer resistance to Musa cultivar Pisanglilin against infestation by Odoiporus longicollis [Oliver] and characterization of Allomones

2021 ◽  
Vol 53 (1) ◽  
pp. 35-52
Author(s):  
K.J Kavitha ◽  
L. Sibina L ◽  
D.A. Evans
Keyword(s):  
Larvicidal Activity ◽  
Column Chromatography ◽  
Simultaneous Action ◽  
Sulfoquinovosyl Diacylglycerol ◽  
Leucine Amino Peptidase ◽  
Total Free Amino Acids ◽  
Preliminary Study ◽  
Cuticle Development ◽  
Moulting Hormone

In bioassay guided extraction of pseudostem powder of Pisanglilin by organic solvents we found the larvicidal activity in acetone extract, whose column chromatography by methanol-chloroform mixture separated the extract into 9-fractions, of which the 8th fraction showed larvicidal activity. Subfractionation of the active fraction by column chromatography resulted in the isolation of two larvicidal molecules [Stigmasterol-3-O-glucoside (SOG) and Sulfoquinovosyl diacylglycerol (SQDG)]. Yield of SOG was 0.002 % and SQDG was 0.005 % and both were highly toxic to O. longicollis larvae with LD50 of 0.40 and 0.378 ppm, respectively. Larvae fed these compounds stopped feeding on third day and died within one week. SOG inhibited the amylase and protease activity of gut and induced histolysis in the mid gut. While SQDG inhibited the leucine amino peptidase and trypsin like serine protease activities, which decreased the content of total free amino acids. Imbalance in the activities of aspartate amino transferase and alanine amino transferase disrupted the aminoacid metabolism and the compound inhibited the activity of tyrosinase (an enzyme involved in cuticle development). SQDG toxicity caused accumulation of 20-hydroxyecdysone, the active moulting hormone in the hemolymph. Simultaneous action of two allomones present in Pisanglilin effectively resisted the attack of endophytic larvae in the pseudostem and thereby conferred resistance against infestation by O.longicollis. Preliminary study by intrapseudostem injection of Pisanglilin extract in susceptible M.paradisiaca cultivar Kappa, gave complete protection to it from attack by this pest, under field condition.

Characterization of column chromatography separated bio-oil obtained from hydrothermal liquefaction of Spirulina

Fuel ◽  
2021 ◽  
Vol 297 ◽  
pp. 120695
Author(s):  
Jiahui Han ◽  
Xing Li ◽  
Shengyan Kong ◽  
Guang Xian ◽  
Hualong Li ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Hydrothermal Liquefaction ◽  
Bio Oil

Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials

2019 ◽  
Vol 964 ◽  
pp. 240-245 ◽  
Author(s):  
Amaliya Rasyida ◽  
Thalyta Rizkha Pradipta ◽  
Sigit Tri Wicaksono ◽  
Vania Mitha Pratiwi ◽  
Yeny Widya Rakhmawati
Keyword(s):  
Sodium Alginate ◽  
Brown Algae ◽  
Extraction Process ◽  
Composition Analysis ◽  
3D Printer ◽  
X Ray Diffraction ◽  
X Ray ◽  
3D Printed ◽  
Preliminary Study

Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

scholarly journals Phenotypic and Genotypic Characterization of Some Lactic Acid Bacteria Isolated from Bee Pollen: A Preliminary Study

2014 ◽  
Vol 33 (1) ◽  
pp. 11-23 ◽  
Author(s):  
Hani BELHADJ ◽  
Daoud HARZALLAH ◽  
Dalila BOUAMRA ◽  
Seddik KHENNOUF ◽  
Saliha DAHAMNA ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bee Pollen ◽  
Genotypic Characterization ◽  
Preliminary Study ◽  
Phenotypic And Genotypic Characterization

scholarly journals Production Process Effect on Mexican Agave Syrups Quality: A Preliminary Study

2018 ◽  
Vol 7 (3) ◽  
pp. 50
Author(s):  
Blanca I. Maldonado-Guevara ◽  
Sandra T. Martín del Campo ◽  
Anaberta Cardador-Martínez
Keyword(s):  
Production Process ◽  
Industrial Process ◽  
Quality Parameters ◽  
Thermal Hydrolysis ◽  
Process Variables ◽  
Anova Analysis ◽  
Hydroxymethyl Furfural ◽  
Wide Range ◽  
Preliminary Study

In this work, the quality parameters of commercial agave syrups produced in five different Mexican states and with different production process were evaluated. Regulated parameters (pH, Moisture, Ashes, and 5-(hydroxymethyl) furfural), as well as color and aw, were measured on 25 agave syrups, including traditional samples as controls. Traditional and semi-industrial syrups were samples obtained by thermal hydrolysis. Additionally, the semi-industrial process included control of process variables such as pH, °Bx, and temperature. The industrial process is technified. The agave syrups ranged from 70-76 °Bx, pH ranged from 3.2-6.7, and moisture from 20.2-28.6%. The aw values shown a wide variation as well as L* a* and b* color parameters. Some of those parameters shown significant differences in ANOVA analysis; however, most of the samples complied with the norm. General Discriminant Analysis (GDA) made it possible to discriminate between production process by using pH, % Ash, b*, 5-(hydroxymethyl)furfural and a* parameters. Further analysis of a wide range of syrups and the inclusion of non-regulated compounds such as volatile compounds and carbohydrates are needed to get more information for a deeper characterization of agave syrups. 

Fabrication and characterization of air-impedance electrospun polydioxanone templates

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Gretchen S. Selders ◽  
Allison E. Fetz ◽  
Shannon L. Speer ◽  
Gary L. Bowlin
Keyword(s):  
Tissue Engineering ◽  
Mercury Porosimetry ◽  
Synthetic Polymers ◽  
Pore Analysis ◽  
Exceptional Value ◽  
Preliminary Study ◽  
Fabrication And Characterization ◽  
Fiber Deposition ◽  
Retention Strength

AbstractElectrospinning, a fabrication technique used to create non-woven, porous templates from natural and synthetic polymers, is commonly used in tissue engineering because it is highly tailorable. However, traditional electrospinning creates restrictive pore sizes that limit the required cell migration. Therefore, tissue engineering groups have sought to enhance and regulate porosity of tissue engineering templates. Air-impedance electrospinning generates templates with tailorable, patterned areas of low and high density fiber deposition. Here we demonstrate an improved air-impedance electrospinning system, consisting of a newly designed funnel equipped to hold changeable porous deposition plates and administer air flow in a confined and focused manner, with parameters that maintain template integrity. In this preliminary study, we quantify the increase in porosity of polydioxanone templates with use of traditional fiber and pore analysis as well as with mercury porosimetry. Additionally, we validate the system’s significance in fabricating enhanced porosity templates that maintain their mechanical properties (i.e. elastic modulus, tensile strength, and suture retention strength) despite the deliberate increase in porosity. This is of exceptional value to the template’s integrity and efficacy as these parameters can be further optimized to induce the desired template porosity, strength, and texture for a given application.

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