Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic differences between stock suspension cultures derived from a single seedling

1975 ◽  
Vol 53 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
The Media ◽  
Isoenzyme Patterns ◽  
Stock Suspension

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon suspension cultures derived from a single seedling. The enzymes studied included malate, glutamate, and glucose-6-phosphate dehydrogenases; peroxidase; polyphenol oxidase; esterase; acid phosphatase; and leucine amino peptidase. Peroxidase isoenzymes were also detected in the media. The isoenzymatic patterns of the three cultures were different for some enzymes, similar for others, and identical for the rest. The isoenzymatic patterns were recorded on a number of occasions over a period of 3 years and they remained relatively unchanged.

Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic changes during the callus culture cycle and differences between stock cultures

1974 ◽  
Vol 52 (12) ◽  
pp. 2621-2629 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Polyphenol Oxidase ◽  
Callus Culture ◽  
Cell Cultures ◽  
Callus Cultures ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
Culture Cycle ◽  
Enzyme Patterns ◽  
Isoenzyme Patterns

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon callus cultures derived from a single seedling. The enzymes studied included peroxidase, polyphenol oxidase, catalase, malate and glutamate dehydrogenases, esterase, and leucine amino peptidase. Enzyme patterns changed during the culture cycle and several isoenzymes appeared only at certain times. The isoenzymatic patterns of the three cultures were very similar but persistent differences between them were observed.

The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (18) ◽  
pp. 2185-2195 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Malate Dehydrogenase ◽  
Leucine Aminopeptidase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glutamate Oxaloacetate Transaminase ◽  
Cotyledon Cell ◽  
Isoenzyme Patterns

Changes in the activity and isoenzyme patterns of acid phosphatase, leucine aminopeptidase, glutamate-oxaloacetate transaminase, esterase, and malate and glutamate dehydrogenases were studied in cotyledon cell suspension cultures of Phaseolus vulgaris grown in the presence and absence of the growth regulators 2,4-dichlorophenoxyacetic acid and kinetin. With all enzymes studied, the pattern of isoenzymes and total enzymatic activity changed with the different phases of the culture cycle. In particular, the patterns of esterase, malate dehydrogenase, and glutamate dehydrogenase changed markedly with the inoculation of cells into fresh medium.The differences in isoenzyme patterns of cells grown with and without regulators were predominantly quantitative. However, certain minor isoenzymes of acid phosphatase, glutamate-oxaloacetate transaminase, esterase, and malate dehydrogenase were only detected in cultures grown in the presence of the regulators, while one isoenzyme of leucine aminopeptidase and two of esterase were unique to cells cultured in the absence of regulators.Three cathodic isoenzymes of acid phosphatase were released from wall material by 1 M NaCl. Such isoenzymes were also detected in the medium and in cytoplasmic extracts. Increase in the wall isoenzymes following inoculation into fresh medium was correlated with a decrease in anodic, cytoplasmic acid phosphatase.

The effect of 2,4-D and kinetin on peroxidase activity and isoenzyme pattern in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1857-1867 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Peroxidase Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Cotyledon Cell ◽  
Cell Growth And Differentiation ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Peroxidase (EC 1.11.1.7) activity was at a minimum during the phase of cell division and at a maximum during the phase of cell expansion. Both the pattern and activity of peroxidase isoenzymes changed during the culture cycle.Cells cultured without growth regulators showed increased peroxidase activity and changed isoenzyme patterns. Certain peroxidase isoenzymes were only present or prominent during specific phases of the culture cycle.The electrophoretic mobilities of peroxidase isoenzymes detected in the medium were not the same as those of the cytoplasmic isoenzymes. Cell cultures grown with and without growth regulators showed different patterns of medium peroxidase activity.Results are discussed in relation to the correlation of peroxidase activity with cell wall expansion and the possible role of peroxidase in cell growth and differentiation.

On the generality of methods to obtain single-cell plant suspension cultures

1978 ◽  
Vol 56 (20) ◽  
pp. 2521-2527 ◽  
Author(s):  
D. J. Kubek ◽  
M. L. Shuler
Keyword(s):  
Single Cell ◽  
Cell Cultures ◽  
Mass Number ◽  
Cell Mass ◽  
Continuous Cultivation ◽  
Suspension Cultures ◽  
Media Composition ◽  
Glycine Max L ◽  
The Media ◽  
Biochemical State

Several techniques employing mechanical, chemical, or enzymatic methods have been suggested for the production of essentially single-cell plant suspension cultures. If single-cell cultures can be obtained, the effects of the media composition on growth can be unambiguously determined. Additionally, such cultures would be amenable to optical and electronic methods for rapidly determining cell mass, number, and volume and could easily be used in experiments on continuous cultivation. Most methods to produce single-cell cultures have been applied to only one or two species. In this paper, these techniques are compared when extended to cultures of Paul's Scarlet Rose (Rosa sp.) cells and soybean cells (Glycine max L.). It is concluded that no technique will generally give sustained disaggregation without affecting the apparent biochemical state of the culture.

Initiation and morphological development of somatic embryoids from barley cell cultures

1984 ◽  
Vol 62 (6) ◽  
pp. 1245-1249 ◽  
Author(s):  
L. S. Kott ◽  
K. J. Kasha
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Cell Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Morphological Development ◽  
Low Concentrations ◽  
The Media ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Barley Cell

Somatic embryogenesis was induced in callus previously initiated from immature embryos of barley. These cultures ranged in age from 6 weeks to 30 months. Embryoids were readily initiated from homogenized suspension-grown aggregates when plated on modified B5 media with 2,4-dichlorophenoxyacetic acid. Low concentrations (0.1 and 0.05 mg∙L−1) of abscisic acid promoted further maturation of embryoids, while gibberellic acid (1 mg∙L−1) and kinetin (0.1 mg∙L−1) were used in the media to encourage embryoid germination. The development of somatic embryoids from initiation through maturation and germination is described.

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