Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic changes during the callus culture cycle and differences between stock cultures

1974 ◽  
Vol 52 (12) ◽  
pp. 2621-2629 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Polyphenol Oxidase ◽  
Callus Culture ◽  
Cell Cultures ◽  
Callus Cultures ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
Culture Cycle ◽  
Enzyme Patterns ◽  
Isoenzyme Patterns

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon callus cultures derived from a single seedling. The enzymes studied included peroxidase, polyphenol oxidase, catalase, malate and glutamate dehydrogenases, esterase, and leucine amino peptidase. Enzyme patterns changed during the culture cycle and several isoenzymes appeared only at certain times. The isoenzymatic patterns of the three cultures were very similar but persistent differences between them were observed.

Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic differences between stock suspension cultures derived from a single seedling

1975 ◽  
Vol 53 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
The Media ◽  
Isoenzyme Patterns ◽  
Stock Suspension

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon suspension cultures derived from a single seedling. The enzymes studied included malate, glutamate, and glucose-6-phosphate dehydrogenases; peroxidase; polyphenol oxidase; esterase; acid phosphatase; and leucine amino peptidase. Peroxidase isoenzymes were also detected in the media. The isoenzymatic patterns of the three cultures were different for some enzymes, similar for others, and identical for the rest. The isoenzymatic patterns were recorded on a number of occasions over a period of 3 years and they remained relatively unchanged.

Changes in the amount and composition of fractions from extracellular polysaccharide during the culture cycle of cotyledon cell suspension culture of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (3-4) ◽  
pp. 198-201 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Suspension Culture ◽  
Cell Suspension Culture ◽  
Cold Water ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
Neutral Sugars ◽  
Culture Cycle

Three different polysaccharides (cold-water-soluble pectin, EDTA-soluble pectin, and neutral polysaccharides) were separated from extracellular polysaccharide produced by bush-bean cotyledon suspension cultures. The different polysaccharides were associated with different phases of the culture cycle. The neutral sugars present in the pectin fractions showed considerable fluctuation during the culture cycle as compared with the rather small changes in the levels of the sugars present in the neutral polysaccharides.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

scholarly journals Effect of Nitrogen and Molybdenum on Post Harvest Soil Characteristics and Economic Yield of Bush Bean (Phaseolus vulgaris L.).

2010 ◽  
pp. 9-14
Author(s):  
MK Hossain ◽  
MS Islam
Keyword(s):  
Phaseolus Vulgaris ◽  
Organic Carbon ◽  
Soil Characteristics ◽  
Ammonium Molybdate ◽  
Research Field ◽  
Treatment Combination ◽  
Net Benefit ◽  
Total N ◽  
Post Harvest ◽  
Bush Bean

A field experiment was carried out at the research field of Sher-e-Bangla Agricultural University, Dhaka during December 2006 to February 2007 to study the effect of nitrogen and molybdenum on post harvest soil characteristics and economic yield of bush bean (Phaseolus vulgaris L.). The treatments consisted of 5 levels of N (0, 40, 80, 120 and 160 kg ha-1) designated and 3 levels of Mo (0, 0.5 and 1.0 kg ha-1). Urea and ammonium molybdate were used as the sources of nitrogen and molybdenum, respectively. The characteristics of the post harvest soils showed a marked variation in relation to soil pH, soil organic carbon, N, P, K and S content in the post harvest soil due to application of N and Mo. The highest organic carbon (0.60%) and total N (0.072%) was obtained from N120. The highest organic carbon (0.60%) and total N (0.068%) was obtained from Mo0.5. The maximum organic carbon was obtained (0.63%) from N120Mo0.5 treatment combination. The maximum total N was obtained (0.075%) from N160Mo0.5 treatment combination. The results of economic analysis showed that the highest net benefit of Tk.1,68,722.00 ha-1 was obtained in N120Mo0.5 treatment and the lowest net benefit of Tk.16,559.00 ha-1 was found in control.

scholarly journals Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L.

2019 ◽  
Vol 20 (7) ◽  
pp. 1787 ◽  
Author(s):  
Muhammad Asad Ullah ◽  
Duangjai Tungmunnithum ◽  
Laurine Garros ◽  
Samantha Drouet ◽  
Christophe Hano ◽  
...  
Keyword(s):  
Callus Culture ◽  
High Performance ◽  
Biological Activities ◽  
Fold Increase ◽  
Dry Weight ◽  
Callus Cultures ◽  
Lepidium Sativum ◽  
Uv C ◽  
Lepidium Sativum L

Lepidium sativum L. is a rich source of polyphenols that have huge medicinal and pharmaceutical applications. In the current study, an effective abiotic elicitation strategy was designed for enhanced biosynthesis of polyphenols in callus culture of L. sativum. Callus was exposed to UV-C radiations for different time intervals and various concentrations of melatonin. Secondary metabolites were quantified by using high-performance liquid chromatography (HPLC). Results indicated the total secondary metabolite accumulation of nine quantified compounds was almost three fold higher (36.36 mg/g dry weight (DW)) in melatonin (20 μM) treated cultures, whereas, in response to UV-C (60 min), a 2.5 fold increase (32.33 mg/g DW) was recorded compared to control (13.94 mg/g DW). Metabolic profiling revealed the presence of three major phytochemicals, i.e., chlorogenic acid, kaemferol, and quercetin, in callus culture of L. sativum. Furthermore, antioxidant, antidiabetic, and enzymatic activities of callus cultures were significantly enhanced. Maximum antidiabetic activities (α-glucosidase: 57.84%; α-amylase: 62.66%) were recorded in melatonin (20 μM) treated callus cultures. Overall, melatonin proved to be an effect elicitor compared to UV-C and a positive correlation in these biological activities and phytochemical accumulation was observed. The present study provides a better comparison of both elicitors and their role in the initiation of physiological pathways for enhanced metabolites biosynthesis in vitro callus culture of L. sativum.

Cyclamen callus culture

1969 ◽  
Vol 47 (12) ◽  
pp. 2065-2067 ◽  
Author(s):  
J. R. Loewenberg
Keyword(s):  
Callus Culture ◽  
Defined Medium ◽  
Callus Cultures ◽  
Cyclamen Persicum

Cyclamen persicum callus cultures have been established on a defined medium. The tissue requires an auxin and a cytokinin. Adenine, while not required, greatly stimulates growth. The callus grows more vigorously in the dark than in light. After more than 6 years of subculture, the callus retains the capacity to form roots and shoots.

Extracellular polysaccharide from cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1972 ◽  
Vol 50 (10) ◽  
pp. 2031-2037 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Higher Plants ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Cell Number ◽  
Suspension Cultures ◽  
Logarithmic Phase ◽  
Bush Bean ◽  
Culture Cycle ◽  
High Yields

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.

Exchange Transaminase Reactions in Bush Bean (Phaseolus vulgaris) Roots

1969 ◽  
Vol 130 (1) ◽  
pp. 23-26 ◽  
Author(s):  
S. Abbadi ◽  
L. M. Shannon
Keyword(s):  
Phaseolus Vulgaris ◽  
Bush Bean

scholarly journals Callus Culture of Thai Basil Is an Effective Biological System for the Production of Antioxidants

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4859 ◽  
Author(s):  
Saher Nazir ◽  
Hasnain Jan ◽  
Duangjai Tungmunnithum ◽  
Samantha Drouet ◽  
Muhammad Zia ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Callus Culture ◽  
Biological System ◽  
Rosmarinic Acid ◽  
Callus Cultures ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Chicoric Acid

Thai basil is a renowned medicinal plant and a rich source of bioactive antioxidant compounds with several health benefits, with actions to prevent of cancer, diabetes and cardiovascular disease. Plant cell and tissue culture technologies can be routinely established as an important, sustainable and low-cost biomass source to produce high-value phytochemicals. The current study aimed at developing an effective protocol to produce Thai basil leaf-derived callus cultures with sustainable and high production of biomass and antioxidants as an alternative of leaves production. MS basal medium with various concentrations of plant growth regulators (PGRs) compatible with nutraceutical applications (i.e., gibberellic acid (GA3) and 6-benzylaminopurine (BAP) either alone or in combination with naphthalene acetic acid (NAA)) were evaluated. Among all tested PGRs, the combination BAP:NAA (5 mg/L:1 mg/L) yields the maximum biomass accumulation (fresh weight (FW): 190 g/L and dry weight (DW): 13.05 g/L) as well as enhanced phenolic (346.08 mg/L) production. HPLC quantification analysis indicated high productions of chicoric acid (35.77 mg/g DW) and rosmarinic acid (7.35 mg/g DW) under optimized callus culture conditions. Antioxidant potential was assessed using both in vitro cell free and in vivo cellular antioxidant assays. Maximum in vitro antioxidant activity DPPH (93.2% of radical scavenging activity) and ABTS (1322 µM Trolox equivalent antioxidant capacity) was also observed for the extracts from callus cultures grown in optimal conditions. In vivo cellular antioxidant activity assay confirmed the effective protection against oxidative stress of the corresponding extract by the maximum inhibition of ROS and RNS production. Compared to commercial leaves, callus extracts showed higher production of chicoric acid and rosmarinic acid associated with higher antioxidant capacity. In addition, this biological system also has a large capacity for continuous biomass production, thus demonstrating its high potential for possible nutraceutical applications.

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