Improvement of polygalacturonase production at high temperature by mixed culture of Aspergillus niger and Saccharomyces cerevisiae

2011 ◽  
Vol 102 (21) ◽  
pp. 10085-10088 ◽  
Author(s):  
Jie-Min Zhou ◽  
Xiang-Yang Ge ◽  
Wei-Guo Zhang
Author(s):  
Astri Nugroho ◽  
Edison Effendi ◽  
Tika Novaria

<p>Limbah padat tapioka merupakan hasil samping dari pengolahan tepung tapioka berupa ampas dan banyak mengandung karbohidrat yang dapat dikembangkan manfaatnya dengan cara mengolah limbah tersebut melalui proses hidrolisis asam dan fermentasi. Penelitian ini bertujuan mengetahui nilai C/N sebagai cara untuk mengetahui laju dekomposisi bahan organik dan mengetahui kadar etanol yang dihasilkan dari proses hidrolisis dan fermentasi pada limbah padat tapioka. Hidrolisis dilakukan dengan menggunakan 1.5% 1M H2SO4. Fermentasi dilakukan dengan variasi mikroorganisme 10% dan 20% secara mixed culture menggunakan Bacillus licheniformis, Aspergillus niger dan Saccharomyces cerevisiae pada limbah padat tapioka 50 gr, 100 gr, 150 gr dan 200 gr. Kadar etanol tertinggi pada variasi mikroorganisme 10% terjadi pada substrat 50 gr jam ke-120 yaitu 4.40% dengan jumlah mikroorganisme 2.29x1017 koloni/ml, pH = 4.60, nilai C/N = 36.43 Pada variasi mikroorganisme 20%, kadar etanol tertinggi terjadi pada substrat 50 gr jam ke-120 yaitu 3.26%, jumlah mikroorganisme 1.82x1017 koloni/ml,             pH = 3.70,  nilai C/N= 49.33. Nilai kinetika pada limbah padat tapioka 50 gr, µ = 0.003206 – 0.009712 1/jam,       µm = 0.00006120 /jam, Ks = 1036.78 mg/1, Y = 23.79, q = 0.000135 – 0.000408 1/jam, YT = 23.79 /jam,               Yobs = 1.8147 – 5.4977 /jam dan Kd = 0.0323 – 0.0388 /jam. Nilai kinetika pada mixed culture 20% pada limbah padat tapioka 50 gr, µ = 0.001818 – 0.008016 1/jam, µm=0.00004288/jam, Ks= 965.94 mg/1, Y = 7.27, q = 0.000250 – 0.001103 1/jam,  YT = 7.27 /jam, Yobs = 0.0961 – 0.4235  /jam dan Kd = 0.1296 – 0.1358 /jam.</p><p>Kata kunci: Limbah padat tapioka, Bioethanol, Fermentasi, Aspergillus niger, Bacillus licheniformis, Saccharomyces cerevisiae </p>


2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guangsen Fan ◽  
Chao Teng ◽  
Dai Xu ◽  
Zhilei Fu ◽  
Pengxiao Liu ◽  
...  

Ethyl acetate content has strong influence on the style and quality of Baijiu. Therefore, this study investigated the effect of Saccharomyces cerevisiae Y3401 on the production of ethyl acetate by Wickerhamomyces anomalus Y3604. Analysis of cell growth showed that Y3401 influences Y3604 by nutrient competition and inhibition by metabolites, while the effect of Y3604 on Y3401 was mainly competition for nutrients. Mixed fermentation with two yeasts was found to produce more ethyl acetate than a single fermentation. The highest yield of ethyl acetate was 2.99 g/L when the inoculation ratio of Y3401:Y3604 was 1:2. Synergistic fermentation of both yeasts improved ethyl acetate production and increased the content of other flavor compounds in liquid and simulated solid-state fermentation for Baijiu. Saccharomyces cerevisiae had a positive effect on ethyl acetate production in mixed culture and provides opportunities to alter the aroma and flavor perception of Baijiu.


2016 ◽  
Vol 3 (1) ◽  
pp. 20-24
Author(s):  
N Lageju ◽  
SC Shahi ◽  
NK Goil

Background and Objectives: Otomycosis is the fungal infection of ear with some complications involving middle ear cavity and mastoid cavity. It is one of commonly encountered problem in otolaryngology clinics. It is commonly present in hot, humid with moisture, high temperature. So, this research is to study the clinical profile of otomycosis in central terrain region of Nepal.Material and Methods: The study was conducted in department of ENT- head and neck surgery in Janaki medical college and teaching hospital from August 2015 to June 2016. All the clinically diagnosed cases were enrolled in the study with typical symptoms and characteristics fungal debris in the external auditory canal (EAC). All the data regarding age, sex, occupation, presenting complaints, type of fugal debris, presence of acute otitis externa, findings of tympanic membrane (TM) taken.Results: Total of 77 patients were enrolled. Among them 27(35.1%) were male and 50(64.9%) were female. The most common presenting complaint is itching followed by earache, ear discharge, hearing loss. Itching was present in 77.9% of the cases followed by earache in 72.7%, aural fullness in 29%, ear discharge in 46.8% and hearing loss in 31.2%. Based on type of fungal debris on otoscopy, blackish was the commonest with frequency of 38 patients (49.4%) followed by whitish debris in 27 patients (35.1%) and yellowish among 12 patients (15.6%).Conclusion: Otomycosis is one of the most common presenting problems in otorhinolaryngology OPD. Females are affected more. Aspergillus niger implicated the most and ear itching is the most common presenting symptom.Janaki Medical College Journal of Medical Sciences (2015) Vol. 3 (1):20-24


Sign in / Sign up

Export Citation Format

Share Document