Simultaneous nitrification and denitrification by a novel isolated Pseudomonas sp. JQ-H3 using polycaprolactone as carbon source

2019 ◽  
Vol 288 ◽  
pp. 121506 ◽  
Author(s):  
Xiujie Wang ◽  
Weiqi Wang ◽  
Yang Zhang ◽  
Zhitao Sun ◽  
Jing Zhang ◽  
...  
2019 ◽  
Vol 80 (8) ◽  
pp. 1512-1523
Author(s):  
Weiwei Ma ◽  
Yuxing Han ◽  
Wencheng Ma ◽  
Hongjun Han ◽  
Chunyan Xu ◽  
...  

Abstract A simultaneous nitrification and denitrification (SND) bioaugmention system with Pseudomonas sp. HJ3 inoculated was established to explore the potential of simultaneous phenol and nitrogen removal in coal gasification wastewater (CGW). When the concentration of influent chemical oxygen demand (COD) and total phenols (TPh) was 1,765.94 ± 27.43 mg/L and 289.55 ± 10.32 mg/L, the average removal efficiency of COD and TPh at the stable operating stage reached 64.07% ± 0.76% and 74.91% ± 0.33%, respectively. Meanwhile, the average removal efficiency of NH4+-N and total nitrogen (TN) reached 67.96% ± 0.17% and 57.95% ± 0.12%, respectively. The maximum SND efficiency reached 83.51%. Furthermore, SND bioaugmentation performed with good nitrification tolerance of phenol shock load and significantly reduced toxic inhibition of organisms. Additionally, the microbial community analysis indicated that Pseudomonas sp. HJ3 was the predominant bacterium in the SND bioaugmentation system. Moreover, the indigenous nitrogen removal bacteria such as Thauera, Acidovorax and Stenotrophomonas were enriched, which further enhanced the nitrogen removal in the SND bioaugmentation system. The results demonstrated the promising application of SND bioaugmentation for enhancing simultaneous phenol and nitrogen removal in CGW treatment.


2014 ◽  
Vol 955-959 ◽  
pp. 618-622
Author(s):  
Li Fang Guo ◽  
Chang Hang Wu

In order to investigate the potential of sequencing batch biofilm reactor (SBBR) technology of two-sludge system for denitrifying dephosphatation (A2ON process),we build a tab-scale experiment .Then find that the simultaneous removal of phosphorus and nitrogen in the two sludge system is possible .By the control of COD/TN ratio ,highly effective simultaneous nitrification and denitrification is achieved in the A2ON system.And the ratio of denitrification is 85%, the ratio of dephosphatation is 87%.It also demonstrates that the process is stable ,at the same time decrease the effect of dephosphorization and denitrification by the limited carbon source.


2012 ◽  
Vol 610-613 ◽  
pp. 2012-2016
Author(s):  
Tao Yang ◽  
Wan You Zhang ◽  
Lan He Zhang ◽  
Pei Ying Wu ◽  
Shuo Yang

A SBR was used to treat power plant sewage based on SND when pH, DO(dissolved oxygen),MLSS, were 7.0-8.0, 0.5-1mg/L, 3000±200mg/L, respectively. Results indicated average removal efficiency of COD and NH4+-N were 89% ,96.34%.Because of low COD/ NH4+-N, average removal efficiency of TN(total nitrogen)was only 71.15%, effect of SND was not apparent. After improving COD/ NH4+-N by adding carbon source intermittently, removal effects of COD and NH4+-N were not reduced, and effluent NO3--N and NO2--N was not accumulated, average removal efficiency of TN was 95%, and efficiency of SND was enhanced obviously. The effluent of system was recycled effectively. It indicates that SBR based on SND is feasible in theory and technology which is applied to treat power plant sewage.


Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty

2013 ◽  
Vol 39 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Katarzyna Bernat

Abstract In this study, the dependence between volumetric exchange rate (n) in an SBR (Sequencing Batch Reactor) with a modified cycle and simultaneous nitrification and denitrification (SND) efficiency during the treatment of anaerobic sludge digester supernatant was determined. In the SBR cycle alternating three aeration phases (with limited dissolved oxygen (DO) concentration up to 0.7 mg O2/L) and two mixing phases were applied. The lengths of each aeration and mixing phases were 4 and 5.5 h, respectively. Independently of n, a total removal of ammonium was achieved. However, at n = 0.1 d-1 and n = 0.3 d-1 nitrates were the main product of nitrification, while at n = 0.5 d-1, both nitrates and nitrites occurred in the effluent. Under these operational conditions, despite low COD/N (ca. 4) ratio in the influent, denitrification in activated sludge was observed. A higher denitrification efficiency at n = 0.5 d-1 (51.3%) than at n = 0.1 d-1 (7.8%) indicated that n was a crucial factor influencing SND via nitrite and nitrate in the SBR with a low oxygen concentration in aeration phases.


2001 ◽  
Vol 43 (1) ◽  
pp. 269-276 ◽  
Author(s):  
N. Puznava ◽  
M. Payraudeau ◽  
D. Thornberg

The aim of this article is to present a new biological aerated filter (BAF) for nitrogen removal based on simultaneous nitrification and denitrification. Contrary to the systems which integrate both an aerated and a non-aerated zone to allow complete nitrogen removal in one compact or two different units (pre-denitrification and nitrification), this upflow BAF system is based on the principle of simultaneous nitrification and denitrification since the filter is completely aerated. The denitrification process is possible due to the diffusion effect which dominates biofilm processes. The real time aeration control allows us to maintain a low dissolved oxygen value (0.5 to 3 mg O2/l). In this case, the biofilm will not be fully (or less) penetrated with oxygen and denitrification will be carried out in a large part of the biofilm. Therefore, nitrification and denitrification is running simultaneously in different depths of the biofilm. By using 50% less air this BAF gave the same results (less than 20mg TN/l) on pilot plant as a classical nitrification and denitrification BAF (Toettrup et al., 1994). Less recirculation was necessary to achieve the same denitrification.


2021 ◽  
Author(s):  
Aline dos Reis Souza ◽  
Mateus Pimentel de Matos ◽  
Luciene Alves Batista Siniscalchi ◽  
Ronaldo Fia

Abstract The objective of this study was to evaluate the effect of the introduction of a complementary aerobic treatment composed of a submerged aerated biological filter (SABF) with a secondary clarifier (SC), followed by horizontal subsurface flow constructed wetlands (CWs), after anaerobic units, on the ability to remove pollutants in different aeration phases (Ph1, Ph2, and Ph3) at the effluent treatment station of the Parque Francisco de Assis (PFA) dog shelter. Ph1 and Ph2 had 7 and 5 hours of daily aeration, respectively, and Ph3 had intermittent aeration every 2 hours. The phases were monitored regarding the removal efficiency of organic matter, solids, nutrients (N, P), coliforms, and detection of Giardia and Cryptosporidium. It was found that post-treatment provided greater removal efficiencies and that the aeration strategy of Ph3 showed mean efficiencies of 71% for COD removal and 77% for BOD removal, being similar or statistically higher, even with less biodegradable effluent, than those of Ph1 and Ph2. The SABF and SC removed N by nitrification and denitrification, leaving a total Kjeldahl nitrogen (TKN) concentration in the effluent of 18 mg L−1. The CW showed potential for simultaneous nitrification and denitrification (SND), in addition to solid filtration. The system did not satisfactorily remove thermotolerant coliforms (ThermC) (1 ± 0 log). PCR suggested the presence of the pathogens Giardia and Cryptosporidium in all post-treatment units in Ph1 and Ph2.


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