AFM and impedance spectroscopy characterization of the immobilization of antibodies on indium–tin oxide electrode through self-assembled monolayer of epoxysilane and their capture of Escherichia coli O157:H7

2005 ◽  
Vol 20 (7) ◽  
pp. 1407-1416 ◽  
Author(s):  
Liju Yang ◽  
Yanbin Li
2011 ◽  
Vol 56 (28) ◽  
pp. 10776-10785 ◽  
Author(s):  
Anand Jain ◽  
Giulio Gazzola ◽  
Aurora Panzera ◽  
Michele Zanoni ◽  
Enrico Marsili

2020 ◽  
Vol 16 ◽  
Author(s):  
Elif Burcu AYDIN ◽  
Muhammet AYDIN ◽  
Mustafa Kemal SEZGİNTÜRK

: In this study, it was aimed to develop a reliable tool based on star-shaped poly(glycidyl methacrylate) polymer coated disposable indium tin oxide electrode for determination of Tumor necrosis factor-alpha (TNF α), an important disease biomarker. Star shaped polymer was used as an interface material for anti-TNF α antibodies immobilization. Anti-TNF α antibodies were immobilized covalently onto polymer coated indium tin oxide electrode. The immunosensor responses were recorded by electrochemical impedance spectroscopy and cyclic voltammetry techniques and electrochemical impedance spectroscopy was utilized for TNF α determination. The suggested immunosensor exhibited a linear range between 0.02 and 4 pg/mL TNF α, and the detection limit was found as 6 fg/mL. Scanning electron microscopy and atomic force microscopy were used for electrode surface characterization. In addition, the suggested immunosensor was used for TNF α sensing in human serum samples. The results displayed recoveries between 97.07 and 100.19%. Moreover, this immunosensor had a simple fabrication procedure and a long storage-stability.


Talanta ◽  
2014 ◽  
Vol 118 ◽  
pp. 224-230 ◽  
Author(s):  
Mina Souiri ◽  
Nesrine Blel ◽  
Dejla Sboui ◽  
Lotfi Mhamdi ◽  
Thibaut Epalle ◽  
...  

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