Single base mismatch detection by microsecond voltage pulses

2005 ◽  
Vol 21 (6) ◽  
pp. 888-893 ◽  
Author(s):  
F. Fixe ◽  
V. Chu ◽  
D.M.F. Prazeres ◽  
J.P. Conde
Keyword(s):  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Voltage Pulses

Label-free visual detection of nucleic acids in biological samples with single-base mismatch detection capability

2012 ◽  
Vol 48 (4) ◽  
pp. 576-578 ◽  
Author(s):  
Yanling Song ◽  
Weiting Zhang ◽  
Yuan An ◽  
Liang Cui ◽  
Chundong Yu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Biological Samples ◽  
Visual Detection ◽  
Label Free ◽  
Detection Capability ◽  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

scholarly journals Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 575 ◽  
Author(s):  
Jialun Han ◽  
Jincai Wu ◽  
Jie Du
Keyword(s):  
Genetic Diseases ◽  
Cost Effective ◽  
Raw 264.7 Cells ◽  
Snp Analysis ◽  
Single Base ◽  
Quenching Effect ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Simple and rapid detection of DNA single base mismatch or point mutation is of great significance for the diagnosis, treatment, and detection of single nucleotide polymorphism (SNP) in genetic diseases. Homogeneous mutation assays with fast hybridization kinetics and amplified discrimination signals facilitate the automatic detection. Herein we report a quick and cost-effective assay for SNP analysis with a fluorescent single-labeled DNA probe. This convenient strategy is based on the efficient quenching effect and the preferential binding of graphene oxide (GO) to ssDNA over dsDNA. Further, a cationic comb-type copolymer (CCC), poly(l-lysine)-graft-dextran (PLL-g-Dex), significantly accelerates DNA hybridization and strand-exchange reaction, amplifying the effective distinction of the kinetic barrier between a perfect matched DNA and a mismatched DNA. Moreover, in vitro experiments indicate that RAW 264.7 cells cultured on PLL-g-Dex exhibits excellent survival and proliferation ability, which makes this mismatch detection strategy highly sensitive and practical.

Preparation of a new electrochemical biosensor for single base mismatch detection in DNA

2013 ◽  
Vol 5 (22) ◽  
pp. 6531 ◽  
Author(s):  
Nasrin Moradi ◽  
Mir Fazlollah Mousavi ◽  
Masoud Ayatollahi Mehrgardi ◽  
Abolhassan Noori
Keyword(s):  
Electrochemical Biosensor ◽  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Rapid Single-Base Mismatch Detection in Genotyping for Phenylketonuria

2003 ◽  
Vol 24 (3) ◽  
pp. 233-242
Author(s):  
Yutaka Takarada ◽  
Shohei Kagawa ◽  
Yoshiyuki Okano ◽  
Takakuni Tanizawa
Keyword(s):  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Detection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters

2017 ◽  
Vol 27 (4) ◽  
pp. 1443-1448 ◽  
Author(s):  
Morteza Hosseini ◽  
Shiva Mohammadi ◽  
Yasaman-Sadat Borghei ◽  
Mohammad Reza Ganjali
Keyword(s):  
Gene Mutation ◽  
P53 Gene ◽  
Silver Nanoclusters ◽  
P53 Gene Mutation ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

scholarly journals Digital-resolution detection of microRNA with single-base selectivity by photonic resonator absorption microscopy

2019 ◽  
Vol 116 (39) ◽  
pp. 19362-19367 ◽  
Author(s):  
Taylor D. Canady ◽  
Nantao Li ◽  
Lucas D. Smith ◽  
Yi Lu ◽  
Manish Kohli ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Resonant Wavelength ◽  
High Signal ◽  
Single Base ◽  
Reflected Light ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Digital Resolution ◽  
Target Probe

Circulating exosomal microRNA (miR) represents a new class of blood-based biomarkers for cancer liquid biopsy. The detection of miR at a very low concentration and with single-base discrimination without the need for sophisticated equipment, large volumes, or elaborate sample processing is a challenge. To address this, we present an approach that is highly specific for a target miR sequence and has the ability to provide “digital” resolution of individual target molecules with high signal-to-noise ratio. Gold nanoparticle tags are prepared with thermodynamically optimized nucleic acid toehold probes that, when binding to a target miR sequence, displace a probe-protecting oligonucleotide and reveal a capture sequence that is used to selectively pull down the target-probe–nanoparticle complex to a photonic crystal (PC) biosensor surface. By matching the surface plasmon-resonant wavelength of the nanoparticle tag to the resonant wavelength of the PC nanostructure, the reflected light intensity from the PC is dramatically and locally quenched by the presence of each individual nanoparticle, enabling a form of biosensor microscopy that we call Photonic Resonator Absorption Microscopy (PRAM). Dynamic PRAM imaging of nanoparticle tag capture enables direct 100-aM limit of detection and single-base mismatch selectivity in a 2-h kinetic discrimination assay. The PRAM assay demonstrates that ultrasensitivity (<1 pM) and high selectivity can be achieved on a direct readout diagnostic.

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