Label-free visual detection of nucleic acids in biological samples with single-base mismatch detection capability

2012 ◽  
Vol 48 (4) ◽  
pp. 576-578 ◽  
Author(s):  
Yanling Song ◽  
Weiting Zhang ◽  
Yuan An ◽  
Liang Cui ◽  
Chundong Yu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Biological Samples ◽  
Visual Detection ◽  
Label Free ◽  
Detection Capability ◽  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Single base mismatch detection by microsecond voltage pulses

2005 ◽  
Vol 21 (6) ◽  
pp. 888-893 ◽  
Author(s):  
F. Fixe ◽  
V. Chu ◽  
D.M.F. Prazeres ◽  
J.P. Conde
Keyword(s):  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Voltage Pulses

scholarly journals Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 575 ◽  
Author(s):  
Jialun Han ◽  
Jincai Wu ◽  
Jie Du
Keyword(s):  
Genetic Diseases ◽  
Cost Effective ◽  
Raw 264.7 Cells ◽  
Snp Analysis ◽  
Single Base ◽  
Quenching Effect ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Simple and rapid detection of DNA single base mismatch or point mutation is of great significance for the diagnosis, treatment, and detection of single nucleotide polymorphism (SNP) in genetic diseases. Homogeneous mutation assays with fast hybridization kinetics and amplified discrimination signals facilitate the automatic detection. Herein we report a quick and cost-effective assay for SNP analysis with a fluorescent single-labeled DNA probe. This convenient strategy is based on the efficient quenching effect and the preferential binding of graphene oxide (GO) to ssDNA over dsDNA. Further, a cationic comb-type copolymer (CCC), poly(l-lysine)-graft-dextran (PLL-g-Dex), significantly accelerates DNA hybridization and strand-exchange reaction, amplifying the effective distinction of the kinetic barrier between a perfect matched DNA and a mismatched DNA. Moreover, in vitro experiments indicate that RAW 264.7 cells cultured on PLL-g-Dex exhibits excellent survival and proliferation ability, which makes this mismatch detection strategy highly sensitive and practical.

Preparation of a new electrochemical biosensor for single base mismatch detection in DNA

2013 ◽  
Vol 5 (22) ◽  
pp. 6531 ◽  
Author(s):  
Nasrin Moradi ◽  
Mir Fazlollah Mousavi ◽  
Masoud Ayatollahi Mehrgardi ◽  
Abolhassan Noori
Keyword(s):  
Electrochemical Biosensor ◽  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Simultaneous Discrimination of Single-Base Mismatch and Full Match Using a Label-Free Single-Molecule Strategy

2018 ◽  
Vol 90 (13) ◽  
pp. 8102-8107 ◽  
Author(s):  
Qiufang Yang ◽  
Tingting Ai ◽  
You Lv ◽  
Yuqin Huang ◽  
Jia Geng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Simultaneous Discrimination ◽  
Label Free ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

Aligner mediated cleavage of nucleic acids for site-specific detection of single base mismatch

Talanta ◽  
2019 ◽  
Vol 201 ◽  
pp. 358-363
Author(s):  
Wufan Pan ◽  
Dongdong Yu ◽  
Yazhou Qin ◽  
Wanghua Wu ◽  
Yuxiang Lu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Specific Detection ◽  
Site Specific ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch
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