A cytokine immunosensor for Multiple Sclerosis detection based upon label-free electrochemical impedance spectroscopy using electroplated printed circuit board electrodes

2009 ◽  
Vol 25 (2) ◽  
pp. 506-509 ◽  
Author(s):  
Kinjal Bhavsar ◽  
Aaron Fairchild ◽  
Eric Alonas ◽  
Daniel K. Bishop ◽  
Jeffrey T. La Belle ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Printed Circuit Board ◽  
Electrochemical Impedance ◽  
Circuit Board ◽  
Label Free ◽  
Printed Circuit

A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy

2007 ◽  
Vol 23 (3) ◽  
pp. 428-431 ◽  
Author(s):  
Jeffrey T. La Belle ◽  
Kinjal Bhavsar ◽  
Aaron Fairchild ◽  
Anusuya Das ◽  
James Sweeney ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Label Free

scholarly journals Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 575 ◽  
Author(s):  
Gorachand Dutta ◽  
Abdoulie A. Jallow ◽  
Debjani Paul ◽  
Despina Moschou
Keyword(s):  
Electrochemical Detection ◽  
Printed Circuit Board ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Charge Transfer Resistance ◽  
Circuit Board ◽  
Label Free ◽  
Electrochemical Impedance Spectra ◽  
Transfer Resistance ◽  
Printed Circuit

This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (Rct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 103 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.

Label-free analysis of water-polluting parasite by electrochemical impedance spectroscopy

2010 ◽  
Vol 25 (5) ◽  
pp. 1122-1129 ◽  
Author(s):  
T. Houssin ◽  
J. Follet ◽  
A. Follet ◽  
E. Dei-Cas ◽  
V. Senez
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Label Free

Nanomonitor Technology for Glycosylation Analysis

2009 ◽  
Vol 1236 ◽  
Author(s):  
Gaurav Chatterjee ◽  
Manish Bothara ◽  
Srivatsa Aithal ◽  
Vinay J Nagraj ◽  
Peter Wiktor ◽  
...  
Keyword(s):  
Double Layer ◽  
Electrical Double Layer ◽  
Printed Circuit Board ◽  
Electrochemical Impedance ◽  
Electrochemical Techniques ◽  
Protein Glycosylation ◽  
Circuit Board ◽  
Biological Macromolecules ◽  
Label Free ◽  
Alumina Membrane

AbstractChanges in protein glycosylation have great potential as markers for the early diagnosis of cancer and other diseases. The current analytical tools for the analysis of glycan structures need expensive instrumentation, advanced expertise, is time consuming and therefore not practical for routine screening of glycan biomarkers from human samples in a clinical setting.We are developing a novel ultrasensitive diagnostic platform called ‘NanoMonitor’ to enable rapid label-free glycosylation analysis. The technology is based on electrochemical impedance spectroscopy where capacitance changes are measured at the electrical double layer interface as a result of interaction of two molecules.The NanoMonitor platform consists of a printed circuit board with array of electrodes forming multiple sensor spots. Each sensor spot is overlaid with a nanoporous alumina membrane that forms a high density of nanowells. Lectins, proteins that bind to and recognize specific glycan structures, are conjugated to the surface of nanowells. When specific glycoproteins from a test sample bind to lectins in the nanowells, it produces a perturbation to the electrical double layer at the solid/liquid interface at the base of each nanowell. This perturbation results in a change in the impedance of the double layer which is dominated by the capacitance changes within the electrical double layer.The nanoscale confinement or crowding of biological macromolecules within the nanowells is likely to enhance signals from the interaction of glycoproteins with the lectins leading to a high sensitivity of detection with the NanoMonitor as compared to other electrochemical techniques.Using a panel of lectins, we were able to detect subtle changes in the glycosylation of fetuin protein as well as differentiate glycoproteins from normal versus cancerous cells. Our results indicate that NanoMonitor can be used as a cost-effective miniature electronic biosensor for the detection of glycan biomarkers.

scholarly journals CortiWatch: watch-based cortisol tracker

2019 ◽  
Vol 5 (9) ◽  
pp. FSO416 ◽  
Author(s):  
Paul Rice ◽  
Sayali Upasham ◽  
Badrinath Jagannath ◽  
Roshan Manuel ◽  
Madhavi Pali ◽  
...  
Keyword(s):  
Printed Circuit Board ◽  
Cost Effective ◽  
Circuit Board ◽  
Electrochemical Technique ◽  
Label Free ◽  
Printed Circuit ◽  
Electrochemical Testing ◽  
Testing Techniques ◽  
Small Form Factor ◽  
A Current

Sweat-based analytics have recently caught the attention of researchers and medical professionals alike because they do not require professionally trained personnel or invasive collection techniques to obtain a sample. The following presents a small form-factor biosensor for reporting physiological ranges of cortisol present in ambient sweat (8–151 ng/ml). This device obtains cortisol measurements through low volumes of unstimulated sweat from the user’s wrist. We designed a potentiostatic circuit on a printed circuit board to perform electrochemical testing techniques. The detection modality developed for quantifying sensor response to varying cortisol concentrations is a current based electrochemical technique, chronoamperometry (CA). From the results, the sensor can detect cortisol in the physiologically relevant ranges of cortisol; thus, the sensor is a noninvasive, label free, cost-effective solution for tracking cortisol levels for circadian diagnostics.

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