High throughput capture of circulating tumor cells using an integrated microfluidic system

2013 ◽  
Vol 47 ◽  
pp. 113-119 ◽  
Author(s):  
Zongbin Liu ◽  
Wang Zhang ◽  
Fei Huang ◽  
Hongtao Feng ◽  
Weiliang Shu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Microfluidic System

scholarly journals High-Throughput Selection, Enumeration, Electrokinetic Manipulation, and Molecular Profiling of Low-Abundance Circulating Tumor Cells Using a Microfluidic System

2011 ◽  
Vol 83 (6) ◽  
pp. 2301-2309 ◽  
Author(s):  
Udara Dharmasiri ◽  
Samuel K. Njoroge ◽  
Małgorzata A. Witek ◽  
Morayo G. Adebiyi ◽  
Joyce W. Kamande ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Molecular Profiling ◽  
Microfluidic System

scholarly journals Arrays of high-aspect ratio microchannels for high-throughput isolation of circulating tumor cells (CTCs)

2013 ◽  
Vol 20 (10-11) ◽  
pp. 1815-1825 ◽  
Author(s):  
Mateusz L. Hupert ◽  
Joshua M. Jackson ◽  
Hong Wang ◽  
Małgorzata A. Witek ◽  
Joyce Kamande ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
High Aspect Ratio

scholarly journals Clinical Validation of an Ultra High-Throughput Spiral Microfluidics for the Detection and Enrichment of Viable Circulating Tumor Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e99409 ◽  
Author(s):  
Bee Luan Khoo ◽  
Majid Ebrahimi Warkiani ◽  
Daniel Shao-Weng Tan ◽  
Ali Asgar S. Bhagat ◽  
Darryl Irwin ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Clinical Validation

scholarly journals Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay

2018 ◽  
Vol 115 (40) ◽  
pp. 9986-9991 ◽  
Author(s):  
Manjima Dhar ◽  
Jeffrey Nam Lam ◽  
Tonya Walser ◽  
Steven M. Dubinett ◽  
Matthew B. Rettig ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Activity Ratio ◽  
Proteolytic Enzymes ◽  
Microfluidic System ◽  
Matrix Metalloprotease ◽  
Rare Cancer ◽  
Companion Diagnostics ◽  
Functional Profiling ◽  
Vortex Capture

Tumor cells are hypothesized to use proteolytic enzymes to facilitate invasion. Whether circulating tumor cells (CTCs) secrete these enzymes to aid metastasis is unknown. A quantitative and high-throughput approach to assay CTC secretion is needed to address this question. We developed an integrated microfluidic system that concentrates rare cancer cells >100,000-fold from 1 mL of whole blood into ∼50,000 2-nL drops composed of assay reagents within 15 min. The system isolates CTCs by size, exchanges fluid around CTCs to remove contaminants, introduces a matrix metalloprotease (MMP) substrate, and encapsulates CTCs into microdroplets. We found CTCs from prostate cancer patients possessed above baseline levels of MMP activity (1.7- to 200-fold). Activity of CTCs was generally higher than leukocytes from the same patient (average CTC/leukocyte MMP activity ratio, 2.6 ± 1.5). Higher MMP activity of CTCs suggests active proteolytic processes that may facilitate invasion or immune evasion and be relevant phenotypic biomarkers enabling companion diagnostics for anti-MMP therapies.

Abstract 1144: High-throughput genetic and expression analysis of circulating tumor cells

2010 ◽  
Author(s):  
AmirAli H. Talasaz ◽  
Gordon Cann ◽  
Zulfiqar Gulzar ◽  
Jing Chen ◽  
Craig April ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Expression Analysis ◽  
High Throughput
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