DNA methyltransferase activity assay based on visible light-activated photoelectrochemical biosensor

2014 ◽  
Vol 53 ◽  
pp. 263-267 ◽  
Author(s):  
Yunlei Zhou ◽  
Zhenning Xu ◽  
Mo Wang ◽  
Bing Sun ◽  
Huanshun Yin ◽  
...  
Keyword(s):  
Visible Light ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Photoelectrochemical Biosensor

Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity

2016 ◽  
Vol 52 (12) ◽  
pp. 2517-2520 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Wei Jiang
Keyword(s):  
Dna Methyltransferase ◽  
Detection System ◽  
Rolling Circle Amplification ◽  
Molecular Probe ◽  
Sensitive Assay ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Fluorescence Detection System

A novel fluorescence detection system is developed for DNA methyltransferase activity assay based on target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy.

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

Immuno-DNA binding directed template-free DNA extension and enzyme catalysis for sensitive electrochemical DNA methyltransferase activity assay and inhibitor screening

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3064-3072 ◽  
Author(s):  
Ying Zhang ◽  
Lijie Hao ◽  
Zhen Zhao ◽  
Xiaoyan Yang ◽  
Li Wang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Enzyme Catalysis ◽  
Dna Methyltransferase ◽  
Dna Sensing ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Inhibitor Screening ◽  
Free Dna ◽  
Sensing Platform ◽  
Template Free

A new electrochemical immuno-DNA sensing platform for DNA methyltransferase activity assay and inhibitor screening.

MoS2nanosheets as an effective fluorescence quencher for DNA methyltransferase activity detection

The Analyst ◽  
2015 ◽  
Vol 140 (9) ◽  
pp. 3210-3215 ◽  
Author(s):  
Huimin Deng ◽  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Dna Methyltransferase ◽  
Van Der Waals ◽  
Van Der Waals Interactions ◽  
Activity Detection ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Fluorescence Quencher

A fluorometric DNA methyltransferase activity assay is described. MoS2nanosheets are employed as the fluorescence quencherviavan der Waals interactions with fluorophore labeled substrate DNA.

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