Development of microfluidic platform capable of high-throughput absolute quantification of single-cell multiple intracellular proteins from tumor cell lines and patient tumor samples

2020 ◽  
Vol 155 ◽  
pp. 112097 ◽  
Author(s):  
Lixing Liu ◽  
Hongyu Yang ◽  
Dong Men ◽  
Meng Wang ◽  
Xiaolei Gao ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Single Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Absolute Quantification ◽  
Tumor Cell Lines ◽  
Microfluidic Platform ◽  
Intracellular Proteins

Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level

Small ◽  
2018 ◽  
Vol 14 (17) ◽  
pp. 1703684 ◽  
Author(s):  
Xiangchun Zhang ◽  
Ru Liu ◽  
Qingming Shu ◽  
Qing Yuan ◽  
Gengmei Xing ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Tumor Cell ◽  
Single Cell ◽  
Cell Lines ◽  
Single Cell Level ◽  
Tumor Cell Lines ◽  
Cell Level ◽  
Invasive Tumor

scholarly journals High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines

2016 ◽  
Vol 34 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Channing Yu ◽  
Aristotle M Mannan ◽  
Griselda Metta Yvone ◽  
Kenneth N Ross ◽  
Yan-Ling Zhang ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Tumor Cell Lines ◽  
Specific Cancer

scholarly journals High-Throughput Flow Cytometric Method for the Simultaneous Measurement of CAR-T Cell Characterization and Cytotoxicity against Solid Tumor Cell Lines

2018 ◽  
Vol 23 (7) ◽  
pp. 603-612
Author(s):  
Emily M. Martinez ◽  
Samuel D. Klebanoff ◽  
Stephanie Secrest ◽  
Gabrielle Romain ◽  
Samuel T. Haile ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Solid Tumor ◽  
Cell Function ◽  
Tumor Cell Lines ◽  
Car T Cell ◽  
Car T

High-throughput flow cytometry is an attractive platform for the analysis of adoptive cellular therapies such as chimeric antigen receptor T cell therapy (CAR-T) because it allows for the concurrent measurement of T cell–dependent cellular cytotoxicity (TDCC) and the functional characterization of engineered T cells with respect to percentage of CAR transduction, T cell phenotype, and measurement of T cell function such as activation in a single assay. The use of adherent tumor cell lines can be challenging in these flow-based assays. Here, we present the development of a high-throughput flow-based assay to measure TDCC for a CAR-T construct co-cultured with multiple adherent tumor cell lines. We describe optimal assay conditions (such as adherent cell dissociation techniques to minimize impact on cell viability) that result in robust cytotoxicity assays. In addition, we report on the concurrent use of T cell transduction and activation antibody panels (CD25) that provide further dissection of engineered T cell function. In conclusion, we present the development of a high-throughput flow cytometry method allowing for in vitro interrogation of solid tumor, targeting CAR-T cell–mediated cytotoxicity, CAR transduction, and engineered T cell characterization in a single assay.

Heterogeneity of radiation induced apoptosis in Ewing Tumor cell lines characterized on a single cell level

APOPTOSIS ◽  
2005 ◽  
Vol 10 (1) ◽  
pp. 177-184 ◽  
Author(s):  
S. K�nemann ◽  
T. B�lling ◽  
A. Kolkmeyer ◽  
D. Riesenbeck ◽  
S. Hesselmann ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Single Cell ◽  
Cell Lines ◽  
Single Cell Level ◽  
Tumor Cell Lines ◽  
Cell Level ◽  
Radiation Induced ◽  
Induced Apoptosis ◽  
Ewing Tumor

Interaction of Human Tumor Cells with Human Platelets and the Coagulation System

1983 ◽  
Vol 50 (03) ◽  
pp. 726-730 ◽  
Author(s):  
Hamid Al-Mondhiry ◽  
Virginia McGarvey ◽  
Kim Leitzel
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Human Platelets ◽  
Factor Vii ◽  
Coagulation System ◽  
Breast Adenocarcinoma ◽  
Activate Factor ◽  
Tumor Cell Lines

SummaryThis paper reports studies on the interaction between human platelets, the plasma coagulation system, and two human tumor cell lines grown in tissue culture: Melanoma and breast adenocarcinoma. The interaction was monitored through the use of 125I- labelled fibrinogen, which measures both thrombin activity generated by cell-plasma interaction and fibrin/fibrinogen binding to platelets and tumor cells. Each tumor cell line activates both the platelets and the coagulation system simultaneously resulting in the generation of thrombin or thrombin-like activity. The melanoma cells activate the coagulation system through “the extrinsic pathway” with a tissue factor-like effect on factor VII, but the breast tumor seems to activate factor X directly. Both tumor cell lines activate platelets to “make available” a platelet- derived procoagulant material necessary for the conversion of prothrombin to thrombin. The tumor-derived procoagulant activity and the platelet aggregating potential of cells do not seem to be inter-related, and they are not specific to malignant cells.

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