Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

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Facile silicification of plastic surface for bioassays

Chemical Communications ◽

10.1039/c6cc09359k ◽

2017 ◽

Vol 53 (13) ◽

pp. 2134-2137 ◽

Cited By ~ 1

Author(s):

Seonki Hong ◽

Ki Soo Park ◽

Ralph Weissleder ◽

Cesar M. Castro ◽

Hakho Lee

Keyword(s):

Nucleic Acid ◽

Surface Chemistry ◽

Point Of Care ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

Plastic Substrates ◽

Biomimetic Surface ◽

Plastic Surface

We report a biomimetic surface chemistry to modify plastic substrates for point-of-care nucleic acid extraction.

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Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

BioMed Research International ◽

10.1155/2017/9306564 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 56

Author(s):

Nasir Ali ◽

Rita de Cássia Pontello Rampazzo ◽

Alexandre Dias Tavares Costa ◽

Marco Aurelio Krieger

Keyword(s):

Nucleic Acid ◽

Extraction Method ◽

Point Of Care ◽

Extraction Methods ◽

Vital Role ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

Advantages And Disadvantages ◽

Point Of Care Diagnostics ◽

Working Principle

Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at thepoint of careorpoint of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.

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Advances in Fully Automated Nucleic Acid Extraction System Based on Magnetic Nanobeads

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2019.3051 ◽

2019 ◽

Vol 11 (12) ◽

pp. 1633-1643

Author(s):

Zhu Chen ◽

Changhu Xiao ◽

Manling Tang ◽

Yuyue Xu ◽

Ziyu He ◽

...

Keyword(s):

Nucleic Acid ◽

High Throughput ◽

Point Of Care ◽

Low Cost ◽

Future Trend ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

Advantages And Disadvantages ◽

Good Repeatability ◽

Magnetic Nanobeads

Using magnetic nanobeads (MNBs) to extract nucleic acids is an efficient, inexpensive, easy automation, high throughput and good repeatability, instead of the traditional nucleic acid extraction (NAE) methods. Advances in fully automated MNBs-based nucleic acid extraction systems (MNAES) can push the frontiers of point-of-care testing (POCT) devices towards low-cost, automation, and enhanced accuracy molecular-level diagnostics. So, this paper introduces the pipettingbased MNAES with position of magnetic separation kit or tip, and based on magnetic bar MNAES with blending manner is shock or rotation. Further, advantages and disadvantages of various MNAES are compared. We envisage that the future trend in molecular diagnosis and monitoring will be facilitation, intelligent, miniaturization, and high throughput MNAES with sample-in-answer-out capability.

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A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

Analytical Methods ◽

10.1039/c3ay40162f ◽

2013 ◽

Vol 5 (13) ◽

pp. 3177 ◽

Cited By ~ 18

Author(s):

Samantha Byrnes ◽

Andy Fan ◽

Jacob Trueb ◽

Francis Jareczek ◽

Mark Mazzochette ◽

...

Keyword(s):

Nucleic Acid ◽

Molecular Diagnostics ◽

Room Temperature ◽

Point Of Care ◽

Storage System ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

And Storage ◽

Pressure Driven

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A fast nucleic acid extraction system for point-of-care and integration of digital PCR

The Analyst ◽

10.1039/c9an01067j ◽

2019 ◽

Vol 144 (23) ◽

pp. 7032-7040 ◽

Cited By ~ 4

Author(s):

Juxin Yin ◽

Jiumei Hu ◽

Jingjing Sun ◽

Ben Wang ◽

Ying Mu

Keyword(s):

Nucleic Acid ◽

Point Of Care ◽

Digital Pcr ◽

Extraction System ◽

Acid Extraction ◽

Nucleic Acid Extraction

This work showcases a PTFE-based nucleic acid extraction system for point-of-care and integration of digital PCR.

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An integrated paper-based sample-to-answer biosensor for nucleic acid testing at the point of care

Lab on a Chip ◽

10.1039/c5lc01388g ◽

2016 ◽

Vol 16 (3) ◽

pp. 611-621 ◽

Cited By ~ 143

Author(s):

Jane Ru Choi ◽

Jie Hu ◽

Ruihua Tang ◽

Yan Gong ◽

Shangsheng Feng ◽

...

Keyword(s):

Nucleic Acid ◽

Point Of Care ◽

Acid Extraction ◽

Nucleic Acid Testing ◽

Point Of Care Testing ◽

Nucleic Acid Extraction

An integrated paper-based sample-to-answer biosensor is proposed, which enables simple paper-based nucleic acid extraction, amplification and detection for point-of-care testing.

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