Differential effects of 1,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3 on protein expression in primary human mesenchymal stem cells from bone marrow (MSCBM): A quantitative proteomics analysis

Bone ◽  
2012 ◽  
Vol 50 ◽  
pp. S70-S71 ◽  
Author(s):  
A.K. Stunes⁎ ◽  
M. Pepaj ◽  
T.S. Steigedal ◽  
U. Syversen ◽  
E.F. Eriksen
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Protein Expression ◽  
Quantitative Proteomics ◽  
Human Mesenchymal Stem Cells ◽  
Proteomics Analysis ◽  
Dihydroxyvitamin D3 ◽  
Differential Effects ◽  
1,25 Dihydroxyvitamin D3

scholarly journals 1,25-Dihydroxyvitamin D3 Treatment Delays Cellular Aging in Human Mesenchymal Stem Cells while Maintaining Their Multipotent Capacity

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e29959 ◽  
Author(s):  
Barbara Klotz ◽  
Birgit Mentrup ◽  
Martina Regensburger ◽  
Sabine Zeck ◽  
Jutta Schneidereit ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cellular Aging ◽  
Dihydroxyvitamin D3 ◽  
Treatment Delays ◽  
1,25 Dihydroxyvitamin D3

scholarly journals Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1592
Author(s):  
Sevil Özer ◽  
H. Seda Vatansever ◽  
Feyzan Özdal-Kurt
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cells ◽  
Hypoxic Condition ◽  
Immunoperoxidase Technique ◽  
Cellular Functions ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition.

Differential Effects of X-Rays and High-Energy 56Fe Ions on Human Mesenchymal Stem Cells

2009 ◽  
Vol 73 (3) ◽  
pp. 869-877 ◽  
Author(s):  
Kyle Kurpinski ◽  
Deok-Jin Jang ◽  
Sanchita Bhattacharya ◽  
Bjorn Rydberg ◽  
Julia Chu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
High Energy ◽  
X Rays ◽  
Differential Effects
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