Kinetics of binding the mRNA cap analogues to the translation initiation factor eIF4E under second-order reaction conditions

The reaction between hexamethylenediisocyanate and 1-pentanol in toluene was studied by means of reversed-phase liquid chromatography. By employing this method, it was possible to determine all components of the reaction mixture including both products, i.e. N-(6-isocyanate hexyl)pentylcarbamate and N,N'-bis(pentyloxycarbonyl)hexamethylenediamine. Relations for the calculation of kinetic constants were derived assuming a competitive consecutive second-order reaction. It was demonstrated that the reaction involved in this case is indeed a second-order reaction, and the rate constants of the first and second consecutive reactions were determined.

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Effects of pH on kinetics of binding of mRNA-cap analogs by translation initiation factor eIF4E

European Biophysics Journal ◽

10.1007/s00249-002-0258-7 ◽

2003 ◽

Vol 31 (8) ◽

pp. 608-616 ◽

Cited By ~ 8

Author(s):

M. Długosz ◽

E. Błachut-Okrasińska ◽

E. Bojarska ◽

E. Darżynkiewicz ◽

J. Antosiewicz

Keyword(s):

Translation Initiation ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Effects Of Ph ◽

Kinetics Of

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Poly(A)‐Binding Protein Affects the Kinetics of Tobacco Etch Virus Pseudoknot RNA Binding to Wheat germ Translation Initiation Factor eIF4F

The FASEB Journal ◽

10.1096/fasebj.22.1_supplement.998.1 ◽

2008 ◽

Vol 22 (S1) ◽

Author(s):

Mateen A Khan ◽

Daniel R Gallie ◽

Dixie J Goss

Keyword(s):

Translation Initiation ◽

Wheat Germ ◽

Rna Binding ◽

Binding Protein ◽

Tobacco Etch Virus ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Kinetics Of

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Structural insights reveal a recognition feature for tailoring hydrocarbon stapled-peptides against the eukaryotic translation initiation factor 4E protein

Chemical Science ◽

10.1039/c8sc03759k ◽

2019 ◽

Vol 10 (8) ◽

pp. 2489-2500 ◽

Cited By ~ 9

Author(s):

Dilraj Lama ◽

Anne-Marie Liberatore ◽

Yuri Frosi ◽

Jessica Nakhle ◽

Natia Tsomaia ◽

...

Keyword(s):

Translation Initiation ◽

Translation Initiation Factor ◽

Binding Kinetics ◽

Initiation Factor ◽

Stapled Peptides ◽

Eukaryotic Translation Initiation Factor ◽

Eukaryotic Translation ◽

Eukaryotic Translation Initiation ◽

Kinetics Of ◽

Structural Insights

We have revealed a non-canonical recognition feature that can modulate the binding kinetics of hydrocarbon stapled-peptides interactions with the eIF4E protein.

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Kinetics and thermodynamics of the reaction between thyroxine and antithyroxine antiserum under the conditions of radioimmunoanalysis

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19822077 ◽

1982 ◽

Vol 47 (8) ◽

pp. 2077-2086

Author(s):

Peter Talán ◽

Juraj Mucha

Keyword(s):

Reaction Rate ◽

Serum Proteins ◽

Relative Rate ◽

Order Reaction ◽

Second Order ◽

Second Order Reaction ◽

Reaction Enthalpy ◽

Thermodynamics And Kinetics ◽

Relative Rate Constants ◽

Kinetics Of

Thermodynamics and kinetics of the reaction between thyroxine and antithyroxine antiserum has been studied using the concentration and temperature conditions usual in the radioimmunoanalysis. The reaction is assumed to be homogeneous and of the second order. Because of the initial concentrations of thyroxine and of the free bonds of the heterogeneous population of antibodies present in the used antiserum the rate constant equation for second-order reaction has been used in the integrated form. The relative rate constants have been obtained and the activation energy of the reaction in absence of the serum proteins in the reaction mixture or, respectively, in the presence of the euthyroidic serum has been calculated. The nature of the effect of so-called deblockators (J) (8-anilino-1-naphthalene sulphonic acid and tiomersal) on the reaction rate and on the change of the reaction enthalpy (or entropy) has also been investigated. The association constant of this reaction has been evaluated from the radioimmunoanalytical data transformed by the Scatchard relation, modified for the conditions of thyroxine radioimmunoanalysis.

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