scholarly journals Unraveling the Energy Transduction Mechanism of Resilin Elasticity-A Combination of Computational and Experimental Study

2014 ◽  
Vol 106 (2) ◽  
pp. 51a ◽  
Author(s):  
Yang Yang ◽  
Xiao Hu
Keyword(s):  
Experimental Study ◽  
Energy Transduction ◽  
Transduction Mechanism

An Investigation into Membrane Bound Redox Carriers Involved in Energy Transduction Mechanism in Brevibacterium linens DSM 20158 with Unsequenced Genome

2014 ◽  
Vol 247 (4) ◽  
pp. 345-355
Author(s):  
Khadija Shabbiri ◽  
Catherine H. Botting ◽  
Ahmad Adnan ◽  
Matthew Fuszard ◽  
Shahid Naseem ◽  
...  
Keyword(s):  
Energy Transduction ◽  
Brevibacterium Linens ◽  
Transduction Mechanism ◽  
Membrane Bound

scholarly journals Impact of an electric field on P-type ATPases

2008 ◽  
Vol 22 (4) ◽  
pp. 319-325 ◽  
Author(s):  
Christian Weidemüller ◽  
Karin Hauser
Keyword(s):  
Electric Field ◽  
Ion Transport ◽  
Transmembrane Helix ◽  
Energy Transduction ◽  
Transmembrane Helices ◽  
Electrical Measurements ◽  
Transduction Mechanism ◽  
Charge Translocation ◽  
P Type ◽  
Hydrolysis Of

P-type ATPases are membrane proteins acting as ion pumps that drive an active transport of cations across the membrane against a concentration gradient. The required energy for the ion transport is provided by binding and hydrolysis of ATP. A reaction mechanism of ion transport and energy transduction is assumed to be common for all P-type ATPases and generally described by the Post-Albers cycle. Transient currents and charge translocation of P-type ATPases were extensively investigated by electrical measurements that apply voltage jumps to initiate the reaction cycle. In this study, we simulate an applied voltage across the membrane by an electric field and perform electrostatic calculations in order to verify the experimentally-driven hypothesis that the energy transduction mechanism is regulated by specific structural elements. Side chain conformational and ionization changes induced by the electric field are evaluated for each transmembrane helix and the selectivity in response is qualitatively analyzed for the Ca2+-ATPase as well as for structural models of the Na+/K+-ATPase. Helix M5 responds with more conformer changes as compared to the other transmembrane helices what is even more emphasized when the stalk region is included. Thus our simulations support experimental results and indicate a crucial role for the highly conserved transmembrane helix M5 in the energy transduction mechanism of P-type ATPases.

scholarly journals An energy transduction mechanism used in bacterial flagellar type III protein export

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Tohru Minamino ◽  
Yusuke V. Morimoto ◽  
Noritaka Hara ◽  
Keiichi Namba
Keyword(s):  
Energy Transduction ◽  
Protein Export ◽  
Transduction Mechanism ◽  
Type Iii

Experimental study on the restoration from a tilt-azimuth series for improvement in resolution

1995 ◽  
Vol 53 ◽  
pp. 636-637
Author(s):  
Norio Baba ◽  
Norihiko Ichise ◽  
Syunya Watanabe
Keyword(s):  
Experimental Study ◽  
Spherical Aberration ◽  
Incident Beam ◽  
Optical Parameters ◽  
Beam Tilting ◽  
Illumination Method ◽  
Restoration Method ◽  
Illumination Mode

The tilted beam illumination method is used to improve the resolution comparing with the axial illumination mode. Using this advantage, a restoration method of several tilted beam images covering the full azimuthal range was proposed by Saxton, and experimentally examined. To make this technique more reliable it seems that some practical problems still remain. In this report the restoration was attempted and the problems were considered. In our study, four problems were pointed out for the experiment of the restoration. (1) Accurate beam tilt adjustment to fit the incident beam to the coma-free axis for the symmetrical beam tilting over the full azimuthal range. (2) Accurate measurements of the optical parameters which are necessary to design the restoration filter. Even if the spherical aberration coefficient Cs is known with accuracy and the axial astigmatism is sufficiently compensated, at least the defocus value must be measured. (3) Accurate alignment of the tilt-azimuth series images.

Artificial cells containing sustainable energy conversion engines

2019 ◽  
Vol 3 (5) ◽  
pp. 573-578 ◽  
Author(s):  
Kwanwoo Shin
Keyword(s):  
Energy Conversion ◽  
Nicotinamide Adenine Dinucleotide ◽  
Sustainable Energy ◽  
Living Cells ◽  
Energy Transduction ◽  
Artificial Cells ◽  
Energy Conversion Process ◽  
Metabolic Reactions ◽  
Metabolic Activities ◽  
Reduced Nicotinamide Adenine Dinucleotide

Living cells naturally maintain a variety of metabolic reactions via energy conversion mechanisms that are coupled to proton transfer across cell membranes, thereby producing energy-rich compounds. Until now, researchers have been unable to maintain continuous biochemical reactions in artificially engineered cells, mainly due to the lack of mechanisms that generate energy-rich resources, such as adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide (NADH). If these metabolic activities in artificial cells are to be sustained, reliable energy transduction strategies must be realized. In this perspective, this article discusses the development of an artificially engineered cell containing a sustainable energy conversion process.

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