scholarly journals Lipopolymer Crowding in Polymer-Tethered Lipid Bilayers Alters Lipid Mixing Behavior and Protein Sequestration in the Presence of Raft-Mimicking Lipid Mixtures

2015 ◽  
Vol 108 (2) ◽  
pp. 87a
Author(s):  
Yifan Ge ◽  
Jiayun Gao ◽  
Amanda P. Siegel ◽  
Noor F. Hussain ◽  
Rainer Jordan ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Mixtures ◽  
Mixing Behavior ◽  
Protein Sequestration

Selective arrangement of vesicles on artificial lipid membrane by biotin-avidin interaction

2021 ◽  
Author(s):  
Kai Hashino ◽  
Daiya Mombayashi ◽  
Yuto Nakatani ◽  
Azusa Oshima ◽  
Masumi Yamaguchi ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Liquid Crystalline ◽  
Lipid Membrane ◽  
Unsaturated Lipid ◽  
Si Substrates ◽  
Phase Domain ◽  
Unsaturated Lipids ◽  
Lipid Mixtures ◽  
The Difference

Abstract Lipid bilayers suspended over microwells on Si substrates are promising platforms for nanobiodevices that mimic cell membranes. Using the biotin-avidin interaction, we have succeeded in selectively arranging vesicles on the freestanding region of a lipid bilayer. When ternary lipid mixtures of saturated lipid, unsaturated lipid, and cholesterol are used, they separate into liquid-order (Lo) and liquid-crystalline (Lα) domains. A freestanding lipid bilayer prefers the Lα-phase over the Lo-phase because of the difference in their flexibility. In addition, the type of biotinylated lipid determines whether it is localized in the Lα-phase domain or the Lo-phase domain. As a result, the biotinylated unsaturated lipids localized in the Lα-phase domain aggregate in the freestanding lipid bilayer, and vesicles labeled with biotin selectively bind to the freestanding lipid bilayer by the biotin-avidin interaction. This technique helps to introduce biomolecules into the freestanding lipid bilayer of nanobiodevices via vesicles.

Solid supported lipid bilayers from artificial and natural lipid mixtures – long-term stable, homogeneous and reproducible

2015 ◽  
Vol 3 (29) ◽  
pp. 6046-6056 ◽  
Author(s):  
Isabelle Möller ◽  
Stefan Seeger
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Deposition Method ◽  
Flow Conditions ◽  
Lipid Mixtures ◽  
Natural Lipid

We show the assembly of reproducible, long-term stable, homogeneous solid supported lipid bilayers under flow conditions by the vesicle deposition method from various artificial and natural lipid mixtures.

scholarly journals Domain Registration in Raft-Mimicking Lipid Mixtures Studied Using Polymer-Tethered Lipid Bilayers

2007 ◽  
Vol 92 (4) ◽  
pp. 1263-1270 ◽  
Author(s):  
Sumit Garg ◽  
Jürgen Rühe ◽  
Karin Lüdtke ◽  
Rainer Jordan ◽  
Christoph A. Naumann
Keyword(s):  
Lipid Bilayers ◽  
Lipid Mixtures

Deposition of Lipid Bilayers with Atomic Force Microscopy

2005 ◽  
Vol 11 (S03) ◽  
pp. 44-47 ◽  
Author(s):  
G. D. Tavares ◽  
M. C. de Oliveira ◽  
J. M. C. Vilela ◽  
M. S. Andrade
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Flat Substrate ◽  
Atomic Force ◽  
Lipid Mixtures ◽  
A Cell ◽  
Integral Proteins ◽  
Spherical Vesicles

Biological membranes are constituted of lipids organized as a two dimensional bilayer supporting peripheral and integral proteins, providing a barrier between the inside and the outside of a cell [1]. Similar membranes can be prepared from the lipid mixtures forming liposomes. The liposomes are multi or unilamellar spherical vesicles in which an aqueous volume is enclosed and can be used to encapsulate some drugs [2]. In order to better expose the details of their structure, these membranes are generally deposited on the surface of a flat substrate. These supported planar lipid membranes can also provide a model system for investigating the properties and functions of the complex cell membrane and membrane mediated processes such as recognition events and biological signal transduction. Various methods have been used to create artificial lipid membranes supported on a solid surface, being the most used the Langmuir-Blodgett monolayers formation [3], the vesicle fusion or liposome adsorption [4] and the solution spreading [5].

scholarly journals Charge-induced phase separation in lipid membranes

Soft Matter ◽  
2014 ◽  
Vol 10 (40) ◽  
pp. 7959-7967 ◽  
Author(s):  
Hiroki Himeno ◽  
Naofumi Shimokawa ◽  
Shigeyuki Komura ◽  
David Andelman ◽  
Tsutomu Hamada ◽  
...  
Keyword(s):  
Phase Separation ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Membrane Phase ◽  
Lipid Mixtures ◽  
The Stability

Phase separation in lipid bilayers is examined. We observed phase-separated structures in various lipid mixtures and determined membrane miscibility temperatures. It was found that a combination of negatively-charged heads and saturation of hydrocarbon tails is dominant for the stability of membrane phase separation.

scholarly journals Crowding-Induced Mixing Behavior of Lipid Bilayers: Examination of Mixing Energy, Phase, Packing Geometry, and Reversibility

Langmuir ◽  
2016 ◽  
Vol 32 (18) ◽  
pp. 4688-4697 ◽  
Author(s):  
Wade F. Zeno ◽  
Alice Rystov ◽  
Darryl Y. Sasaki ◽  
Subhash H. Risbud ◽  
Marjorie L. Longo
Keyword(s):  
Lipid Bilayers ◽  
Mixing Energy ◽  
Mixing Behavior

scholarly journals Effect of cholesterol on permeability of carbon dioxide across lipid membranes

2020 ◽  
Author(s):  
M.C. Blosser ◽  
J. So ◽  
M.S. Madani ◽  
N. Malmstadt
Keyword(s):  
Carbon Dioxide ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Gas Transport ◽  
Gas Diffusion ◽  
Physiological Processes ◽  
Lipid Mixtures ◽  
Model Lipid Membranes ◽  
Order Of Magnitude ◽  
Membrane Components

AbstractDetermining the permeability of lipid membranes to gases is important for understanding the biological mechanisms of gas transport. Experiments on model membranes have been used to determine the permeability of lipid bilayers in the absence of proteins. Previous measurements have used a number of different methods and obtained widely varying results. We have developed a microfluidic based microscopy assay that measures the rate of CO2 permeation in Giant Unilamellar Vesicles (GUVs), and we report permeability data for the POPC-cholesterol system. We find that cholesterol has a strong effect on permeability; bilayers containing high levels of cholesterol are an order of magnitude less permeable than bilayers without cholesterol, 9.9 ± 1.0 x 10−4 cm/s vs. 9.6 ± 1.4 x 10−3 cm/s.Statement of SignificanceDiffusion of dissolved gasses such as carbon dioxide through cell membranes is an important step in physiological processes. Key to understanding the behavior in cells is the measurement of gas diffusion through model lipid membranes, which isolates the effect of the lipids from other membrane components and allows for control of the composition. Previous measurements have yielded different results for the magnitude of gas transport, and have disagreed on the amount that cholesterol affects transport. The present study presents new data on gas transport across lipid mixtures containing cholesterol, and develops a microfluidic assay for gas transport that will enable further work.

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