scholarly journals Using Site Specific Fluorescent Probes to Examine Replication Fork Destabilization by Regulatory Proteins of the Bacteriophage T4 DNA Replication Complex

2017 ◽  
Vol 112 (3) ◽  
pp. 314a-315a
Author(s):  
Davis Jose ◽  
Miya Mary Michael ◽  
Wonbae Lee ◽  
Thomas H. Steinberg ◽  
Andrew H. Marcus ◽  
...  
Keyword(s):  
Dna Replication ◽  
Fluorescent Probes ◽  
Replication Fork ◽  
Bacteriophage T4 ◽  
Regulatory Proteins ◽  
Site Specific ◽  
Replication Complex

scholarly journals Dual Functions of Single-stranded DNA-binding Protein in Helicase Loading at the Bacteriophage T4 DNA Replication Fork

2004 ◽  
Vol 279 (18) ◽  
pp. 19035-19045 ◽  
Author(s):  
Yujie Ma ◽  
Tongsheng Wang ◽  
Jana L. Villemain ◽  
David P. Giedroc ◽  
Scott W. Morrical
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Replication Fork ◽  
Binding Protein ◽  
Bacteriophage T4 ◽  
Dna Binding Protein ◽  
Single Stranded Dna ◽  
Helicase Loading ◽  
Dual Functions

scholarly journals Protein-Protein and Protein-DNA Interactions at the Bacteriophage T4 DNA Replication Fork

1996 ◽  
Vol 271 (45) ◽  
pp. 28045-28051 ◽  
Author(s):  
Daniel J. Sexton ◽  
Theodore E. Carver ◽  
Anthony J. Berdis ◽  
Stephen J. Benkovic
Keyword(s):  
Dna Replication ◽  
Replication Fork ◽  
Bacteriophage T4 ◽  
Dna Interactions ◽  
Protein Dna Interactions

scholarly journals The Escherichia coli Tus–Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Nicolai B. Larsen ◽  
Ehud Sass ◽  
Catherine Suski ◽  
Hocine W. Mankouri ◽  
Ian D. Hickson
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Replication Fork ◽  
Site Specific

scholarly journals Function and Assembly of the Bacteriophage T4 DNA Replication Complex

2003 ◽  
Vol 278 (28) ◽  
pp. 25435-25447 ◽  
Author(s):  
Emmanuelle Delagoutte ◽  
Peter H. von Hippel
Keyword(s):  
Dna Replication ◽  
Bacteriophage T4 ◽  
Replication Complex

scholarly journals RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Maria Manosas ◽  
Senthil K. Perumal ◽  
Piero R. Bianco ◽  
Felix Ritort ◽  
Stephen J. Benkovic ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Single Molecule ◽  
Replication Fork ◽  
Genetic Recombination ◽  
Bacteriophage T4 ◽  
General Mechanism ◽  
Dna Unwinding ◽  
Replication Forks ◽  
Displacement Reactions

Abstract Helicases that both unwind and rewind DNA have central roles in DNA repair and genetic recombination. In contrast to unwinding, DNA rewinding by helicases has proved difficult to characterize biochemically because of its thermodynamically downhill nature. Here we use single-molecule assays to mechanically destabilize a DNA molecule and follow, in real time, unwinding and rewinding by two DNA repair helicases, bacteriophage T4 UvsW and Escherichia coli RecG. We find that both enzymes are robust rewinding enzymes, which can work against opposing forces as large as 35 pN, revealing their active character. The generation of work during the rewinding reaction allows them to couple rewinding to DNA unwinding and/or protein displacement reactions central to the rescue of stalled DNA replication forks. The overall results support a general mechanism for monomeric rewinding enzymes.

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