scholarly journals Characterizing E. coli Phosphoenolpyruvate Carboxykinase Conformational States through Small Angle X-Ray Scattering

2018 ◽  
Vol 114 (3) ◽  
pp. 26a
Author(s):  
Greg L. Hura ◽  
Henry Y.H. Tang ◽  
John A. Tainer
Keyword(s):  
Small Angle ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Conformational States ◽  
X Ray ◽  
E Coli ◽  
X Ray Scattering ◽  
Ray Scattering

scholarly journals Use of small-angle X-ray scattering to resolve intracellular structure changes ofEscherichia colicells induced by antibiotic treatment

2016 ◽  
Vol 49 (6) ◽  
pp. 2210-2216 ◽  
Author(s):  
A. R. von Gundlach ◽  
V. M. Garamus ◽  
T. M. Willey ◽  
J. Ilavsky ◽  
K. Hilpert ◽  
...  
Keyword(s):  
Small Angle ◽  
X Ray ◽  
E Coli ◽  
X Ray Scattering ◽  
Structure Changes ◽  
Average Size ◽  
The Impact ◽  
Antibiotic Agents ◽  
Intracellular Structure ◽  
Ray Scattering

The application of small-angle X-ray scattering (SAXS) to wholeEscherichia colicells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components ofE. coliwas developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins.E. coliwas studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.

Structural investigations of E. Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking

2016 ◽  
Vol 61 (3) ◽  
pp. 414-420 ◽  
Author(s):  
L. A. Dadinova ◽  
E. V. Rodina ◽  
N. N. Vorobyeva ◽  
S. A. Kurilova ◽  
T. I. Nazarova ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Small Angle ◽  
Dihydrolipoamide Dehydrogenase ◽  
Structural Investigations ◽  
X Ray ◽  
E Coli ◽  
X Ray Scattering ◽  
Ray Scattering

Structural stability of E. coli trigger factor studied by synchrotron small-angle X-ray scattering

2014 ◽  
Vol 195 ◽  
pp. 1-7 ◽  
Author(s):  
Yi Shi ◽  
Masaji Shinjo ◽  
Jun-Mei Zhou ◽  
Hiroshi Kihara
Keyword(s):  
Structural Stability ◽  
Small Angle ◽  
Trigger Factor ◽  
X Ray ◽  
E Coli ◽  
X Ray Scattering ◽  
Ray Scattering

A Revised O2 Reduction Model in Li-O2 Batteries as Revealed by in Situ Small Angle X-Ray Scattering

2019 ◽  
Author(s):  
Christian Prehal ◽  
Aleksej Samojlov ◽  
Manfred Nachtnebel ◽  
Manfred Kriechbaum ◽  
Heinz Amenitsch ◽  
...  
Keyword(s):  
Small Angle ◽  
Wide Angle ◽  
Reduction Mechanism ◽  
Reduction Model ◽  
X Ray ◽  
X Ray Scattering ◽  
O2 Reduction ◽  
Ray Scattering

<b>Here we use in situ small and wide angle X-ray scattering to elucidate unexpected mechanistic insights of the O2 reduction mechanism in Li-O2 batteries.<br></b>

In situ Time-Resolved Small-Angle X-ray Scattering Reveals the Formation Kinetics of Polyelectrolyte Complex Micelles

2019 ◽  
Author(s):  
Hao Wu ◽  
Jeffrey Ting ◽  
Siqi Meng ◽  
Matthew Tirrell
Keyword(s):  
Small Angle ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Polyelectrolyte Complex ◽  
Time Resolved ◽  
X Ray ◽  
X Ray Scattering ◽  
Kinetics Of ◽  
Ray Scattering

We have directly observed the <i>in situ</i> self-assembly kinetics of polyelectrolyte complex (PEC) micelles by synchrotron time-resolved small-angle X-ray scattering, equipped with a stopped-flow device that provides millisecond temporal resolution. This work has elucidated one general kinetic pathway for the process of PEC micelle formation, which provides useful physical insights for increasing our fundamental understanding of complexation and self-assembly dynamics driven by electrostatic interactions that occur on ultrafast timescales.

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