scholarly journals Tuning the Baird Aromatic Triplet State Energy of Cyclooctatetraene to Maximize the Self-Healing Mechanism in Organic Fluorophores

2021 ◽  
Vol 120 (3) ◽  
pp. 114a
Author(s):  
Avik K. Pati ◽  
Ouissam El Bakouri ◽  
Steffen Jockusch ◽  
Zhou Zhou ◽  
Roger B. Altman ◽  
...  
Keyword(s):  
Triplet State ◽  
State Energy ◽  
The Self ◽  
Self Healing ◽  
Organic Fluorophores ◽  
Triplet State Energy

scholarly journals Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores

2020 ◽  
Vol 117 (39) ◽  
pp. 24305-24315
Author(s):  
Avik K. Pati ◽  
Ouissam El Bakouri ◽  
Steffen Jockusch ◽  
Zhou Zhou ◽  
Roger B. Altman ◽  
...  
Keyword(s):  
Triplet State ◽  
Large Scale ◽  
State Energy ◽  
Conformational Dynamics ◽  
The Self ◽  
Detection Methods ◽  
Wide Field ◽  
Self Healing ◽  
Organic Fluorophores ◽  
Triplet State Energy

Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. “Self-healing” dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen species that promote phototoxicity. Here, we systematically examine the self-healing mechanism in cyanine-class organic fluorophores spanning the visible spectrum. We show that the Baird aromatic triplet-state energy of cyclooctatetraene can be physically altered to achieve order of magnitude enhancements in fluorophore brightness and signal-to-noise ratio in both the presence and absence of oxygen. We leverage these advances to achieve direct measurements of large-scale conformational dynamics within single molecules at submillisecond resolution using wide-field illumination and camera-based detection methods. These findings demonstrate the capacity to image functionally relevant conformational processes in biological systems in the kilohertz regime at physiological oxygen concentrations and shed important light on the multivariate parameters critical to self-healing organic fluorophore design.

Room temperature phosphorimetric determination of cyanide based on triplet state energy transfer

2003 ◽  
Vol 491 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Marı́a Teresa Fernández-Argüelles ◽  
José M Costa-Fernández ◽  
Rosario Pereiro ◽  
Alfredo Sanz-Medel
Keyword(s):  
Energy Transfer ◽  
Triplet State ◽  
Room Temperature ◽  
State Energy ◽  
Triplet State Energy

Development of Luminescent Terbium(III) Chelates for Protein Labelling: Effect of triplet-state energy level

1997 ◽  
Vol 80 (2) ◽  
pp. 372-387 ◽  
Author(s):  
Harri Takalo ◽  
Veli-Matti Mukkala ◽  
Liisa Meriö ◽  
Juan Carlos Rodríguez-Ubis ◽  
Rosa Sedano ◽  
...  
Keyword(s):  
Energy Level ◽  
Triplet State ◽  
State Energy ◽  
Protein Labelling ◽  
Triplet State Energy

scholarly journals Transfer of Triplet State Energy in Fluid Solutions. IV. Determination of Triplet Yields by the Measurement of Sensitized Biacetyl Phosphorescence.

1969 ◽  
Vol 23 ◽  
pp. 2815-2829 ◽  
Author(s):  
Kjell Sandros ◽  
O. Bastiansen ◽  
Tove Motzfeldt ◽  
D. Heinegård ◽  
Alexandru T. Balaban ◽  
...  
Keyword(s):  
Triplet State ◽  
State Energy ◽  
Triplet State Energy
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