Micro-injection of propranolol within basolateral amygdala impaired fear and spatial memory and dysregulated evoked responses of CA1 neurons following foot shock stress in rats

2021 ◽  
Vol 177 ◽  
pp. 12-21
Author(s):  
Gholam Hossein Meftahi ◽  
Zohreh Jangravi ◽  
Maryam Taghdir ◽  
Mojtaba Sepandi ◽  
Zahra Bahari
Keyword(s):  
Spatial Memory ◽  
Basolateral Amygdala ◽  
Evoked Responses ◽  
Ca1 Neurons ◽  
Shock Stress

scholarly journals Effects of the stimulation of β2-adrenoceptors of the the basolateral amygdala on anxiety-like behaviors following electrical foot-shock stress in male rat

2021 ◽  
Vol 23 (2) ◽  
pp. 250-259
Author(s):  
Gholam Hossein Meftahi ◽  
Gila Pirzad Jahromi ◽  
Nazila Gholipour ◽  
Zahra Bahari ◽  
◽  
...  
Keyword(s):  
Basolateral Amygdala ◽  
Male Rat ◽  
Shock Stress ◽  
Stimulation Of

scholarly journals Evaluation of the effects of L-Theanine on neurobehavior in an adult male Sprague-Dawley Rat Model of PTSD

2019 ◽  
Vol 6 (3) ◽  
pp. 299-308
Author(s):  
John E Buonora ◽  
Patrick M Krum ◽  
Tomás Eduardo Ceremuga
Keyword(s):  
Spatial Memory ◽  
Traumatic Event ◽  
Forced Swim Test ◽  
The United States ◽  
Traumatic Experiences ◽  
Stress Model ◽  
Sprague Dawley ◽  
Post Traumatic Stress ◽  
Post Traumatic ◽  
Shock Stress

Post-traumatic stress disorder (PTSD) continues to be one of the most common mental health disorders in the United States and may occur in response to traumatic experiences. Currently, there are no interventions that prevent the development of PTSD. L-Theanine (L-Th), a major compound in green tea has been found to decrease anxiety and prevent memory impairment and may have potential effects in the prevention of PTSD. Sixty rats were divided into six experimental groups: control vehicle, control L-Th, control naïve, PTSD vehicle, PTSD Pre-L-Th (prophylactic), PTSD Post-L-Th (non-prophylactic). PTSD was induced by a 3-day restraint/tail shock stress model. The effects of L-Th on neurobehavior were evaluated by Elevated Plus-Maze (EPM), Morris Water Maze (MWM), and Forced Swim Test (FST). Our study found that the total food intake weight of PTSD Pre-L-Th (prophylactic) rats were significantly increased compared to that of PTSD vehicle rats (p = .04). Administration of L-Th 24 hours before the initial PTSD event or for 10 days following the last PTSD stress event did not statistically improve mean open arm exploration on the EPM, spatial memory, and learning in the MWM or behavioral despair measured by the FST (p > 0.05). Although the 3-day restraint/tail shock stress model caused stress in the rodents, it did not produce reported PTSD-like anxiety and depression or spatial memory loss. The effect of Pre-L-Th or Post-L-Th treatment, on the neurobehavioral functions could not be effectively evaluated. However, this study provides a foundation for future studies to try different rodent PTSD models to induce PTSD-like neurobehavioral impairments to explore dosage, frequency, as well as the duration of L-Th administration before and/or after the post-traumatic event. The 3-day restraint/tail shock stress model caused stress in the rodents, Pre-L-Theanine treatment preconditioned the PTSD rats to endure stress.

scholarly journals Repeated shock stress facilitates basolateral amygdala synaptic plasticity through decreased cAMP-specific phosphodiesterase type IV (PDE4) expression

2017 ◽  
Vol 223 (4) ◽  
pp. 1731-1745 ◽  
Author(s):  
Steve Ryan ◽  
Chenchen Li ◽  
Aurélie Menigoz ◽  
Rimi Hazra ◽  
Joanna Dabrowska ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Basolateral Amygdala ◽  
Type Iv ◽  
Phosphodiesterase Type ◽  
Shock Stress

scholarly journals Differential effects of acute and repeated stress on hippocampus and amygdala inputs to the nucleus accumbens shell

2013 ◽  
Vol 16 (9) ◽  
pp. 2013-2025 ◽  
Author(s):  
Kathryn M. Gill ◽  
Anthony A. Grace
Keyword(s):  
Nucleus Accumbens ◽  
Basolateral Amygdala ◽  
Acute Stress ◽  
Evoked Responses ◽  
Nucleus Accumbens Shell ◽  
Afferent Pathways ◽  
Repeated Stress ◽  
Repeated Restraint Stress ◽  
The Impact

Abstract The basolateral amygdala (BLA) and ventral subiculum (vSub) of the hippocampus convey emotion and context information, respectively, to the nucleus accumbens (NAc). Using in vivo extracellular recordings from NAc neurons, we examined how acute and repeated restraint stress alters the plasticity of the vSub and BLA afferent pathways. High-frequency (HFS) and low-frequency (LFS) stimulation was applied to the vSub to assess the impact on NAc responses to vSub and BLA inputs. In addition, iontophoretic application of the dopamine D2-antagonist sulpiride was used to explore the role of dopamine in the NAc in mediating the effects of stress on plasticity. Acute and repeated restraint caused disparate effects on BLA- and vSub-evoked responses in the NAc. Following repeated restraint, but not after acute restraint, HFS of the vSub failed to potentiate the vSub–NAc pathway while instead promoting a long-lasting reduction of the BLA–NAc pathway and these effects were independent of D2-receptor activity. In contrast, LFS to the vSub pathway after acute restraint resulted in potentiation in the vSub–NAc pathway while BLA-evoked responses were unchanged. When sulpiride was applied prior to LFS of the vSub after acute stress, there was a pronounced decrease in vSub-evoked responses similar to control animals. This work provides new insight into the impact of acute and repeated stress on the integration of context and emotion inputs in the NAc. These data support a model of stress whereby the hippocampus is inappropriately activated and dominates the information processing within this circuit via a dopaminergic mechanism after acute bouts of stress.

scholarly journals A novel mechanism for amplification of sensory responses by the amygdala-TRN projections

2019 ◽  
Author(s):  
Mark Aizenberg ◽  
Solymar Rolon Martinez ◽  
Tuan Pham ◽  
Winnie Rao ◽  
Julie Haas ◽  
...  
Keyword(s):  
Basolateral Amygdala ◽  
Auditory Pathway ◽  
Emotional Learning ◽  
Evoked Responses ◽  
Sensory Stimuli ◽  
Environmental Signals ◽  
Neuropsychological Disorders ◽  
Sensory Responses ◽  
Evoked Activity ◽  
Central Auditory Pathway

AbstractMany forms of behavior require selective amplification of neuronal representations of relevant environmental signals. Following emotional learning, sensory stimuli drive enhanced responses in the sensory cortex. However, the brain circuits that underlie emotionally driven control of the sensory representations remain poorly understood. Here we identify a novel pathway between the basolateral amygdala (BLA), an emotional learning center in the mouse brain, and the inhibitory nucleus of the thalamus (TRN). We demonstrate that activation of this pathway amplifies sound-evoked activity in the central auditory pathway. Optogenetic activation of BLA suppressed spontaneous, but not tone-evoked activity in the auditory cortex (AC), effectively amplifying tone-evoked responses in AC. Anterograde and retrograde viral tracing identified robust BLA projections terminating at TRN. Optogenetic activation of amygdala-TRN pathway mimicked the effect of direct BLA activation, amplifying tone-evoked responses in the auditory thalamus and cortex. The results are explained by a computational model of the thalamocortical circuitry. In our model, activation of TRN by BLA suppresses spontaneous activity in thalamocortical cells, and as a result, thalamocortical neurons are primed to relay relevant sensory input. These results demonstrate a novel circuit mechanism for shining a neural spotlight on behaviorally relevant signals and provide a potential target for treatment of neuropsychological disorders, in which emotional control of sensory processing is disrupted.

scholarly journals In-vivo evidence for proximodistal heterogeneity in hippocampal CA1 and CA3 during non-spatial memory retrieval

2020 ◽  
Author(s):  
Shihpi Ku ◽  
Erika Atucha ◽  
Nico Alavi ◽  
Magdalena Sauvage
Keyword(s):  
Spatial Memory ◽  
Spatial Information ◽  
Memory Task ◽  
Early Gene ◽  
Support Vector ◽  
Study Phase ◽  
Spatial Network ◽  
Ca1 Neurons ◽  
Odor Memory

Recent immediate early gene evidence suggests that proximal CA3 (proxCA3, close to dentate gyrus) and distal CA1 (distCA1, close to subiculum) form a specialized non-spatial hippocampal subnetwork (nakamura et al, JON, 2013; Beer and Vavra, Plos Biology, 2018) while distal CA3 (distCA3) and proximal CA1 (proxCA1) are more specialized in spatial information processing (Flashbeck et al, 2018). However, direct in-vivo evidence for such functional networks are still missing. Here, we used chronically implanted multi-tetrode recording technique to simultaneously record along the proximodistal axis of the two CA-fields while rats performed a high-demanding delayed non-match to odor memory task. In this task, rats smelled 10 (old) odors during the study phase, and after a 20-minute delay memory for the studied odors was tested by exposing rats to the same odors intermixed with 10 new odors. We recorded 193 CA3- and 367 CA1-neurons in 5 animals who could perfom above threshold (75%). Using Support Vector Machine (SVM) we tested whether proxCA3-distCA1 neurons (non-spatial network) can differentiate the old from new odors better than distCA3-proxCA1 neurons (spatial network). We found that activity in the proxCA3-distCA1 network was relevant for the discrimination between old from new odors and similar to behavior; in contrast, the activity of the distCA3-proxCA1 network was not. Further, we found a gradient in the distribution of task-relevant neurons along the transverse axis of CA1 as well as CA3. Overall, we provide clear in vivo electrophysiological evidence that supports the role of proxCA3-distCA1 network in non-spatial memory processing.

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