Mesenchymal stem cells combined with an artificial dermal substitute improve repair in full-thickness skin wounds

Burns ◽  
2012 ◽  
Vol 38 (8) ◽  
pp. 1143-1150 ◽  
Author(s):  
Dilmar Leonardi ◽  
Daniel Oberdoerfer ◽  
Marilda C. Fernandes ◽  
Rosalva T. Meurer ◽  
Gustavo A. Pereira-Filho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dermal Substitute ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Wu Minjuan ◽  
Xiong Jun ◽  
Shao Shiyun ◽  
Xu Sha ◽  
Ni Haitao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Amniotic Membrane ◽  
Functional Restoration ◽  
Skin Wounds ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Early repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated and cultured adipose derived mesenchymal stem cells (ADMSCs) onto the human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to the surface of AAM and grew well 24 h after seeding. Comparison of the wound healing rate at days 7, 14, and 28 after transplantation showed that ADMSCs seeded on AAM facilitated the healing of full-thickness skin wounds more effectively as compared with either hAM or AAM alone, indicating that ADMSCs participated in skin regeneration. More importantly, we noticed a phenomenon of hair follicle development during the process of skin repair. Composite ADMSCs and AAM not only promoted the healing of the mouse full-thickness defects but also facilitated generation of the appendages of the affected skin, thus promoting restoration of the skin function. Our results provide a new possible therapy idea for the treatment of skin wounds with respect to both anatomical regeneration and functional restoration.

scholarly journals Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

2013 ◽  
Vol 6 (1) ◽  
pp. 12-25 ◽  
Author(s):  
Hend Shafik Basiouny ◽  
Nagla Mohamed Salama ◽  
Zeinab Mohamed El Maadawi ◽  
Eman Abas Farag
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Skin Wounds ◽  
Full Thickness ◽  
Albino Rat ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model

IUBMB Life ◽  
2015 ◽  
Vol 67 (9) ◽  
pp. 701-709 ◽  
Author(s):  
Somia H. Abd-Allah ◽  
Amal S. El-Shal ◽  
Sally M. Shalaby ◽  
Eman Abd-Elbary ◽  
Nehad F. Mazen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Concentrated Hypoxia-Preconditioned Adipose Mesenchymal Stem Cell-Conditioned Medium Improves Wounds Healing in Full-Thickness Skin Defect Model

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Biao Sun ◽  
Shilei Guo ◽  
Fei Xu ◽  
Bin Wang ◽  
Xiujuan Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Skin Defect ◽  
Full Thickness ◽  
Defect Model ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Freeze Dried ◽  
Adipose Mesenchymal Stem Cells

In recent years, the bioactive factors were utilized in exercise and athletic skin injuries. In this research, the concentrated conditioned medium of hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The conditioned medium of hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of protein in freeze-dried conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated hypoxia-preconditioned conditioned medium can reduce the wound size and accelerate the skin wound healing. The concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for wound care in clinical application.

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