scholarly journals The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model

IUBMB Life ◽  
2015 ◽  
Vol 67 (9) ◽  
pp. 701-709 ◽  
Author(s):  
Somia H. Abd-Allah ◽  
Amal S. El-Shal ◽  
Sally M. Shalaby ◽  
Eman Abd-Elbary ◽  
Nehad F. Mazen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Concentrated Hypoxia-Preconditioned Adipose Mesenchymal Stem Cell-Conditioned Medium Improves Wounds Healing in Full-Thickness Skin Defect Model

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Biao Sun ◽  
Shilei Guo ◽  
Fei Xu ◽  
Bin Wang ◽  
Xiujuan Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Skin Defect ◽  
Full Thickness ◽  
Defect Model ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Freeze Dried ◽  
Adipose Mesenchymal Stem Cells

In recent years, the bioactive factors were utilized in exercise and athletic skin injuries. In this research, the concentrated conditioned medium of hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The conditioned medium of hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of protein in freeze-dried conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated hypoxia-preconditioned conditioned medium can reduce the wound size and accelerate the skin wound healing. The concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for wound care in clinical application.

Studies on the Efficacy of Single and Twice Application of Mesenchymal Stem Cells in Full Thickness Cutaneous Wound Healing

2021 ◽  
Author(s):  
Jyotsana Bhatt ◽  
Amarpal . ◽  
Abas Rashid Bhat ◽  
Anuj Pratap Singh ◽  
A.M. Pawde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Full Thickness ◽  
Cutaneous Wound ◽  
Group Iii ◽  
Group I ◽  
Thickness Skin ◽  
Early Wound

Background: A lot of research has been done in the field of wound healing utilising stem cell therapy. This study was conducted to compare the efficacy of single and twice application of mesenchymal stem cells for cutaneous wound healing.Methods: The present study was done on 18 guinea pigs and a 2.5 X 2.5 cm2 full-thickness skin wound was created on the dorsum of each under standard anesthetic protocol. Animals were divided into 3 groups having 6 animals in each. The animals of group I were administered phosphate buffer saline (PBS) subcutaneously on day 0, group II received 1×106 mesenchymal stem cells (MSCs) in PBS subcutaneously on day 0 and group III received 1×106 MSCs in PBS subcutaneously on day 0 and again at day 3, at wound margins. Clinical, photographic, histopathological and histochemical parameters were recorded for each animal.Result: An overall gross and histopathological evaluation suggested early wound closer, better tissue granulation, early orientation of fibrocytes and collagen fibers, revealing a superior quality healing in animals of group III as compared to rest of the groups. From the above study it was concluded that twice application of MSCs leads to faster and qualitatively better healing. 

scholarly journals Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Wu Minjuan ◽  
Xiong Jun ◽  
Shao Shiyun ◽  
Xu Sha ◽  
Ni Haitao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Amniotic Membrane ◽  
Functional Restoration ◽  
Skin Wounds ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Early repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated and cultured adipose derived mesenchymal stem cells (ADMSCs) onto the human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to the surface of AAM and grew well 24 h after seeding. Comparison of the wound healing rate at days 7, 14, and 28 after transplantation showed that ADMSCs seeded on AAM facilitated the healing of full-thickness skin wounds more effectively as compared with either hAM or AAM alone, indicating that ADMSCs participated in skin regeneration. More importantly, we noticed a phenomenon of hair follicle development during the process of skin repair. Composite ADMSCs and AAM not only promoted the healing of the mouse full-thickness defects but also facilitated generation of the appendages of the affected skin, thus promoting restoration of the skin function. Our results provide a new possible therapy idea for the treatment of skin wounds with respect to both anatomical regeneration and functional restoration.

scholarly journals Lentiviral-mediated overexpression of homeobox A4 by human umbilical cord mesenchymal stem cells repairs full-thickness skin defects

2015 ◽  
Vol 11 (5) ◽  
pp. 3517-3522
Author(s):  
LING HE ◽  
HUAI-JUN TU ◽  
WEN-FENG HE ◽  
LING-LING GUO ◽  
SONG-XIA YU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Mesenchymal stem cells combined with an artificial dermal substitute improve repair in full-thickness skin wounds

Burns ◽  
2012 ◽  
Vol 38 (8) ◽  
pp. 1143-1150 ◽  
Author(s):  
Dilmar Leonardi ◽  
Daniel Oberdoerfer ◽  
Marilda C. Fernandes ◽  
Rosalva T. Meurer ◽  
Gustavo A. Pereira-Filho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dermal Substitute ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Experimental evaluation of the effectiveness of adipose mesenchymal stem cells in full-thickness skin wounds

2021 ◽  
Vol 9 (2) ◽  
pp. 175-181
Author(s):  
Ilya V. Artsimovich ◽  
Evgenii V. Zinoviev ◽  
Aleksander V. Gostimskii ◽  
Marat S. Asadulaev ◽  
Sergey V. Vissarionov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Control Group ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Healing Index ◽  
Thickness Skin

BACKGROUND: Currently, numerous techniques or medical devices that allow complete or partial restoration of the lost skin within a short time remain as subjects of development. Many studies have shown the effectiveness of using stem cells in the treatment of full-thickness skin defects, but their use remains very limited. At present, there is no consensus among researchers about the advisability of the use of stem cells in the treatment of burns as well as about the method of their introduction. AIM: This study aimed to examine the possibility of accelerating the reparative histogenesis of tissues in the zone of deep skin burns using cultures of adipogenic mesenchymal cells, as well as to evaluate the effectiveness of various methods of introducing cultures of these cells into the defect zone. MATERIALS AND METHODS: An experimental study was carried out on small laboratory animals (rats). After simulating a grade III burn, mesenchymal stem cells were transplanted and superficially applied to the wound surface or injected under the fascia. In the control group, no wound treatment was performed. To assess the effectiveness of the wound-healing preparations, the appearance of wounds was assessed daily, the nature of the discharge and presence and type of granulations were noted, and the timing of scab rejection and wound healing was recorded. The wound area was assessed using a planimetric method. A histological examination of wound biopsies was carried out on days 7, 14, 21, and 28 of observation. RESULTS: The application of adipogenic mesenchymal stem cells demonstrated the greatest efficiency on the developed burn model. Subfascial administration was less effective, but this method achieved a significant acceleration of wound healing in comparison with the control group. An increase in the healing index by 56.6% demonstrates the highest intensity of reparative regeneration in animals applied with adipogenic mesenchymal stem cells. CONCLUSIONS: The preliminary results show that the application of adipogenic mesenchymal stem cells on the skin defect is more effective than subfascial administration based on the healing index. The use of adipogenic mesenchymal stem cells may substantially increase the effectiveness of the treatment of full-thickness skin defects.

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