Physicochemical and mechanical characterization of bacterial cellulose produced with an excellent productivity in static conditions using a simple fed-batch cultivation strategy

2010 ◽  
Vol 82 (1) ◽  
pp. 173-180 ◽  
Author(s):  
Omer Shezad ◽  
Salman Khan ◽  
Taous Khan ◽  
Joong Kon Park
Keyword(s):  
Bacterial Cellulose ◽  
Mechanical Characterization ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Cultivation Strategy ◽  
Static Conditions ◽  
Fed Batch Cultivation

Enhanced cutinase production of Thermobifida fusca by a two-stage batch and fed-batch cultivation strategy

2009 ◽  
Vol 14 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Gangqiang He ◽  
Guanghua Huo ◽  
Liming Liu ◽  
Yang Zhu ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Batch Cultivation ◽  
Thermobifida Fusca ◽  
Fed Batch ◽  
Two Stage ◽  
Cultivation Strategy ◽  
Fed Batch Cultivation

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

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