cultivation strategy
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Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 316
Author(s):  
Xiaoxiao Yan ◽  
Yongdi Wen ◽  
Menghua Hu ◽  
Zhenqiang Wu ◽  
Xiaofei Tian

Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.


2021 ◽  
Vol 13 ◽  
pp. 297-300
Author(s):  
Zhou Yan ◽  
Shihan Liu ◽  
Ruibing Yuan ◽  
Guiyin Zhang ◽  
Siyuan Liu ◽  
...  

Starting from various problems existing in college students' innovation and entrepreneurship activities, this paper explores the similarities between craftsman spirit and innovation and entrepreneurship ability, analyzes the significance of their combination for social development, and puts forward the integration of craftsman spirit and innovation and entrepreneurship in theoretical teaching, innovation and entrepreneurship practice and psychological teaching. And through some specific methods to achieve the relevant theory, improve the talent training system in colleges and universities, cultivate students' "artisan spirit", and enhance the ability of innovation and entrepreneurship.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefan Kittler ◽  
Christoph Slouka ◽  
Andreas Pell ◽  
Roman Lamplot ◽  
Mihail Besleaga ◽  
...  

AbstractIn many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated. This cultivation mode was found to facilitate stable protein expression using microbial hosts, however mechanistic knowledge of this cultivation strategy is scarce. In this contribution we present a method workflow to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3) exclusively based on bioengineering methods.


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