How the molecular weight affects the in vivo fate of exogenous hyaluronan delivered intravenously: A stable-isotope labelling strategy

AbstractIdentification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical to understanding the molecular mechanisms that underpin key physiological processes and pathologies. Here we report that a brief period of pulsed SILAC diet (Stable Isotope Labelling by Amino acids in Cell culture) enables determination of biological functions corresponding to actively translating proteins in the mouse brain. Our data demonstrate that the hippocampus, cortex and cerebellum are highly active sites of protein synthesis, rapidly expressing key mediators of nutrient sensing and lipid metabolism, as well as critical regulators of synaptic function, axon guidance, and circadian entrainment. Together, these findings confirm that protein metabolic activity varies significantly between brain regions in vivo and indicate that pSILAC-based approaches can identify specific anatomical sites and biological pathways likely to be suitable for drug targeting in neurodegenerative disorders.AbbreviationsApoA1: Apolipoprotein A1, ApoA4: Apolipoprotein A4, ApoE: Apolipoprotein E, ApoJ/Clu: Apolipoprotein J/Clusterin, App: Amyloid-β precursor/A4 protein: App, HDL: high density lipoprotein, Lrp1: Low density lipoprotein receptor-related protein 1, pSILAC: pulsed SILAC, pSIVOM: pulsed-SILAC in vivo labelling in mouse, SILAC: Stable Isotope Labelling by Amino acids in Cell culture)

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Stable isotope labelling of phosphatidylcholine synthesis in vivo in children with acute lung injury

Archives of Disease in Childhood ◽

10.1136/adc.2010.186338.93 ◽

2010 ◽

Vol 95 (Suppl 1) ◽

pp. A43.1-A43

Author(s):

K Goss ◽

L Ward ◽

V Ledger ◽

G Koster ◽

P Townsend ◽

...

Keyword(s):

Acute Lung Injury ◽

Stable Isotope ◽

Lung Injury ◽

Stable Isotope Labelling ◽

Isotope Labelling ◽

Phosphatidylcholine Synthesis

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Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC–MS/MS

Nature Protocols ◽

10.1038/s41596-018-0102-x ◽

2019 ◽

Vol 14 (2) ◽

pp. 313-330 ◽

Cited By ~ 26

Author(s):

Min Yuan ◽

Daniel M. Kremer ◽

He Huang ◽

Susanne B. Breitkopf ◽

Issam Ben-Sahra ◽

...

Keyword(s):

Stable Isotope ◽

Carbon Metabolism ◽

Ex Vivo ◽

Central Carbon Metabolism ◽

Stable Isotope Labelling ◽

Isotope Labelling ◽

Central Carbon

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Using high-resolution Twin-Ion Metabolite Extraction (HiTIME) mass spectrometry with stable isotope labelling to investigate the metabolism of valproic acid in vivo

International Journal of Mass Spectrometry ◽

10.1016/j.ijms.2019.116187 ◽

2019 ◽

Vol 444 ◽

pp. 116187 ◽

Cited By ~ 2

Author(s):

Michael G. Leeming ◽

Noel Cranswick ◽

Christine E. Wright ◽

James Ziogas ◽

Terence J. O'Brien ◽

...

Keyword(s):

Mass Spectrometry ◽

Valproic Acid ◽

High Resolution ◽

Stable Isotope ◽

Stable Isotope Labelling ◽

Isotope Labelling ◽

Metabolite Extraction

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Shifts in rotifer life history in response to stable isotope enrichment: testing theories of isotope effects on organismal growth

Royal Society Open Science ◽

10.1098/rsos.160810 ◽

2017 ◽

Vol 4 (3) ◽

pp. 160810 ◽

Cited By ~ 5

Author(s):

Elena Gorokhova

Keyword(s):

Stable Isotope ◽

Brachionus Plicatilis ◽

Isotope Effects ◽

Experimental Studies ◽

Isotope Enrichment ◽

Heavy Isotope ◽

Material Transfer ◽

Stable Isotope Labelling ◽

Isotope Labelling

In ecology, stable isotope labelling is commonly used for tracing material transfer in trophic interactions, nutrient budgets and biogeochemical processes. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism growth and metabolism. This assumption is, however, challenged by theoretical considerations and experimental studies on kinetic isotope effects in vivo . Here, I demonstrate profound changes in life histories of the rotifer Brachionus plicatilis fed 15 N-enriched algae (0.4–5.0 at%); i.e. at the enrichment levels commonly used in ecological studies. These findings support theoretically predicted effects of heavy isotope enrichment on growth, metabolism and ageing in biological systems and underline the importance of accounting for such effects when using stable isotope labelling in experimental studies.

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