Preparation of chitooligosaccharides from fungal waste mycelium by recombinant chitinase

To isolate recombinant chitinase quickly and boost its anti-fungi activities in vitro, functional magnetic nanometer carrier was used to immobilize recombinant chitinase from the crude enzyme solution and immobilized recombinant chitinase was applied to test whether it would inhibit the growth of gray mold from fruits. In this study, the carboxyl magnetic carrier was produced by solvent thermal reduction method and characterized by scanning electron microscope (SEM) and fourier transform infrared spectrometer (FTIR). Then, the carboxyl magnetic carrier activated by EDC/NHS was applied to immobilize recombinant chitinase and the immobilization efficiency was investigated by quantitative analysis. To obtain the highest immobilization efficiency, reaction conditions were optimized through combining different pH, temperature and reaction period. The results show that the surface of magnetic carrier was successfully carboxyl and the average diameter was 200nm. The immobilization efdiciency could reach the peak 64.43% after 7h reaction at the condition of pH 6 and 25°C. It also shows that immobilized recombinant chitinase can significantly inhibit the growth of gray mold isolated from table grape compared with the enzyme without immobilization with magnetic nanometer carrier.

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Production of Penicillin with Waste Mycelium of Penicillium chrysogenum as the Sole Source of Nitrogen

Applied Microbiology ◽

10.1128/aem.9.3.252-255.1961 ◽

1961 ◽

Vol 9 (3) ◽

pp. 252-255 ◽

Cited By ~ 2

Author(s):

D. Ghosh ◽

B. N. Ganguli

Keyword(s):

Penicillium Chrysogenum ◽

Sole Source ◽

Waste Mycelium

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Research Progress for Gypsum Retarder

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1015.272 ◽

2014 ◽

Vol 1015 ◽

pp. 272-275

Author(s):

Xiao Qiong Ren ◽

Ya Nan Wu ◽

Ying Li ◽

Mo Han Lin ◽

Feng Qing Zhao

Keyword(s):

Organic Acids ◽

Mechanical Strength ◽

Building Materials ◽

Setting Time ◽

Strength Loss ◽

Raw Material ◽

Research Progress ◽

High Quality ◽

Protein Rich ◽

Waste Mycelium

Gypsum retarder is an important additive of building materials. Commercial gypsum retarders are mainly composed of four types: organic acids, phosphates, protein and composite retarder. Although the effects of organic acids and phosphates have better retarding performances, there is still obvious a shortcoming that the mechanical strength reduces largely with time going by. Protein based retarders prolong the setting time of hemihydrates with less strength loss and higher costs. The gypsum retarder from waste mycelium, a protein-rich raw material, solved the problem of the disposal of waste mycelium from anti-biotic plants, while producing high-quality retarder for gypsum, shows potential for future.

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