Identification of chemosensory genes by antennal transcriptome analysis and expression profiles of odorant-binding proteins in parasitoid wasp Aulacocentrum confusum

Abstract Background The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms Pinus sylvestris var. mongolica Litv.. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. Recently, the two woodwasps species were found attacking the P. sylvestris var. mongolica Litv in succession. The olfactory system is the foundation of insect behavior. Olfactory genes were identified through antennal transcriptome analysis. The expression profiles odorant binding proteins (OBPs) were analyzed with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified from the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnocGR2, SnocIR7 in S. noctilio and SnitGR9, SnitGR11, SnitIR17 in S. nitobei. In total, 14 OBPs were expressed primarily in the antennae. SnocOBP9 and SnitOBP9, identified as PBP homologues, were sex-biased expression in two siricid, but with different pattern. SnocOBP11 and SnitOBP11 were highly expressed in antennae and clearly expressed in external genitalia. SnocOBP7 and SnitOBP7 were highly expressed in male genitalia. SnocOBP3 and SnocOBP10 were highly expressed in female genitalia and male heads, while SnitOBP3 and SnitOBP10 did not show obvious tissue bias. Conclusion We analyzed 86 and 91 olfactory genes from S. noctilio and S. nitobei, respectively. Most of the olfactory genes identified were homologous, but also some species-specific olfactory genes were identified, which indicated the similarities and differences of the molecular mechanisms between the two closely-related species. Different expression in the antennae, external genitals or heads, exhibiting an obvious sex bias, suggested their different role in recognizing sex pheromones or plant volatiles. Species-specific expression for several OBPs genes may suggest that they strengthened or lost their original function during species differentiation, resulting in olfactory differences between the two species.

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Antennal transcriptome analysis and expression profiles of odorant binding proteins in two woodwasps, Sirex noctilio and S. nitobei (Hymenoptera: Siricidae)

10.21203/rs.2.9384/v1 ◽

2019 ◽

Author(s):

Bing Guo ◽

Jing-Zhen Wang ◽

Hai-Li Qiao ◽

Wei-Wei Wu ◽

Jing-Jiang Zhou ◽

...

Keyword(s):

Transcriptome Analysis ◽

Olfactory System ◽

Native Species ◽

Binding Proteins ◽

Expression Profiles ◽

External Genitalia ◽

Sirex Noctilio ◽

Odorant Binding Proteins ◽

Odor Recognition ◽

Odorant Binding

Abstract Background The olfactory system is the foundation of insect behavior. Odorant binding proteins (OBPs) are key components of the insect olfactory system. The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms members of the Pinus genus. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. To gain insights into the olfactory mechanisms of these two woodwasp species, olfactory genes were identified using antennal transcriptome analysis. We also analyzed the expression profiles of OBPs with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified in the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnitGR9, and SnitIR17. In total, 14 OBPs (7 in S. noctilio and 7 in S. nitobei) were expressed primarily in the antennae of the two woodwasps. SnocOBP11 and SnitOBP11 were highly expressed in antennae and were also clearly expressed in the external genitalia. SnocOBP3 is highly expressed in the genitalia of females, and SnocOBP7 and SnitOBP7 are highly expressed in the genitalia of males. Meanwhile, SnocOBP10 was specifically expressed in male heads. Conclusion In total, 86 olfactory proteins were identified in S. noctilio, and 91 were identified in S. nitobei. Most SnocOBPs and SnitOBPs displayed enriched expression in the antennae, which are involved in odor recognition. A few OBPs were mainly expressed in the external genitals or heads and exhibited an obvious sex bias, which may indicate that the external genitals and heads are able to recognize sex pheromones or plant volatile compounds as a part of normal behaviors such as feeding, mating, or spawning. Our study provides key insights regarding the mechanism of interactions between the insect olfactory system and specific odor molecules.

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Antennal transcriptome analysis and expression profiles of odorant binding proteins in two woodwasps, Sirex noctilio and S. nitobei (Hymenoptera: Siricidae)

10.21203/rs.2.9384/v2 ◽

2019 ◽

Author(s):

Bing Guo ◽

Jing-Zhen Wang ◽

Hai-Li Qiao ◽

Wei-Wei Wu ◽

Jing-Jiang Zhou ◽

...

Keyword(s):

Transcriptome Analysis ◽

Olfactory System ◽

Native Species ◽

Binding Proteins ◽

Expression Profiles ◽

External Genitalia ◽

Sirex Noctilio ◽

Odorant Binding Proteins ◽

Odor Recognition ◽

Odorant Binding

Abstract Background The olfactory system is the foundation of insect behavior. Odorant binding proteins (OBPs) are key components of the insect olfactory system. The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms members of the Pinus genus. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. To gain insights into the olfactory mechanisms of these two woodwasp species, olfactory genes were identified using antennal transcriptome analysis. We also analyzed the expression profiles of OBPs with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified in the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnitGR9, and SnitIR17. In total, 14 OBPs (7 in S. noctilio and 7 in S. nitobei) were expressed primarily in the antennae of the two woodwasps. SnocOBP11 and SnitOBP11 were highly expressed in antennae and were also clearly expressed in the external genitalia. SnocOBP3 is highly expressed in the genitalia of females, and SnocOBP7 and SnitOBP7 are highly expressed in the genitalia of males. Meanwhile, SnocOBP10 was specifically expressed in male heads. Conclusion In total, 86 olfactory proteins were identified in S. noctilio, and 91 were identified in S. nitobei. Most SnocOBPs and SnitOBPs displayed enriched expression in the antennae, which are involved in odor recognition. A few OBPs were mainly expressed in the external genitals or heads and exhibited an obvious sex bias, which may indicate that the external genitals and heads are able to recognize sex pheromones or plant volatile compounds as a part of normal behaviors such as feeding, mating, or spawning. Our study provides key insights regarding the mechanism of interactions between the insect olfactory system and specific odor molecules.

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Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Insect Science ◽

10.1111/1744-7917.12709 ◽

2019 ◽

Vol 27 (5) ◽

pp. 1019-1030 ◽

Cited By ~ 1

Author(s):

Ling Wang ◽

Ying‐Dong Bi ◽

Ming Liu ◽

Wei Li ◽

Miao Liu ◽

...

Keyword(s):

Binding Proteins ◽

Expression Profiles ◽

Soybean Aphid ◽

Aphis Glycines ◽

Odorant Binding Proteins ◽

Odorant Binding

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Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus

Bulletin of Entomological Research ◽

10.1017/s0007485321000857 ◽

2021 ◽

pp. 1-11

Author(s):

Yu Pan ◽

Xinxin Zhang ◽

Zhun Wang ◽

Lizhong Qi ◽

Xinsheng Zhang ◽

...

Keyword(s):

Membrane Proteins ◽

Sensory Neuron ◽

Binding Proteins ◽

Mate Location ◽

Lissorhoptrus Oryzophilus ◽

Neuron Membrane ◽

Odorant Binding Proteins ◽

Chemosensory Proteins ◽

Chemosensory Genes ◽

Odorant Binding

Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future.

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