Effect of specimen storage conditions on newborn dried blood spots used to assess Toxoplasma gondii immunoglobulin M (IgM)

2011 ◽  
Vol 412 (5-6) ◽  
pp. 455-459 ◽  
Author(s):  
Joanne V. Mei ◽  
LiXia Li ◽  
Sonja A. Rasmussen ◽  
Sarah Collier ◽  
Jaime L. Frias ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Immunoglobulin M ◽  
Blood Spots

scholarly journals Comparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study

2021 ◽  
Vol 43 (05) ◽  
pp. 351-356
Author(s):  
Bárbara Araújo Marques ◽  
Ericka Vianna Machado Carellos ◽  
Vânia Maria Novato Silva ◽  
Fernando Henrique Pereira ◽  
Maria Regina Lage Guerra ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Prenatal Screening ◽  
Dried Blood Spots ◽  
Population Based ◽  
Minas Gerais ◽  
Immunoglobulin M ◽  
Screening Tests ◽  
Reference Test ◽  
Screening Programs ◽  
Blood Spots

Abstract Objective Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil. Methods A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzyme-linked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program. Results The dried blood spot test was able to discriminate positive and negative results of pregnant women when compared with the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM). Conclusion Dried blood samples are easy to collect, store, and transport, and they have a good performance, making this a promising method for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.

Should phosphatidylethanol be currently analysed using whole blood, dried blood spots or both?

2019 ◽  
Vol 57 (5) ◽  
pp. 617-622 ◽  
Author(s):  
Van Long Nguyen ◽  
Michael Fitzpatrick
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Ethanol Metabolism ◽  
Clinical Use ◽  
Blood Spots ◽  
Comparative Review ◽  
And Storage

Abstract Phosphatidylethanol (PEth) are phospholipids produced through non-oxidative ethanol metabolism. They accumulate in red blood cells and have been traditionally analysed in whole blood as potential biomarkers for moderate to long-term alcohol consumption. More recently, their analysis in dried blood spots has been gaining favour, namely, due to the ease in sampling, transport and storage conditions required. This paper aims at providing a short comparative review between analysing PEth in whole blood and dried blood spots and the potential pitfalls that researchers may face when setting up PEth testing for clinical use.

scholarly journals Recovery of Anti-Toxoplasma gondii Immunoglobulin M in Stored Guthrie Card Blood Spots

2009 ◽  
Vol 47 (8) ◽  
pp. 2626-2628 ◽  
Author(s):  
H.-K. Tan ◽  
E. Petersen ◽  
L. N. Moller ◽  
P. Phillips ◽  
E. C. Neto ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin M ◽  
Guthrie Card ◽  
Blood Spots

scholarly journals Stability of gametocyte-specific Pfs25-mRNA in dried blood spots on filter paper subjected to different storage conditions

2012 ◽  
Vol 11 (1) ◽  
Author(s):  
Michael Pritsch ◽  
Andreas Wieser ◽  
Victor Soederstroem ◽  
David Poluda ◽  
Teferi Eshetu ◽  
...  
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Blood Spots

scholarly journals Dried Blood Spots versus Sera for Detection of Rubella Virus-Specific Immunoglobulin M (IgM) and IgG in Samples Collected during a Rubella Outbreak in Peru

2007 ◽  
Vol 14 (11) ◽  
pp. 1522-1525 ◽  
Author(s):  
Rita F. Helfand ◽  
Cesar Cabezas ◽  
Emily Abernathy ◽  
Carlos Castillo-Solorzano ◽  
Ana Cecilia Ortiz ◽  
...  
Keyword(s):  
Filter Paper ◽  
Rubella Virus ◽  
Dried Blood Spots ◽  
Immunoglobulin M ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Specific Immunoglobulin ◽  
Blood Spot

ABSTRACT Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.

Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots

Glycobiology ◽  
2019 ◽  
Vol 29 (12) ◽  
pp. 817-821 ◽  
Author(s):  
Jelena Simunovic ◽  
Marija Vilaj ◽  
Irena Trbojevic-Akmacic ◽  
Ana Momcilovic ◽  
Frano Vuckovic ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dried Blood Spots ◽  
Ultra Performance Liquid Chromatography ◽  
Storage Conditions ◽  
Sampling Procedure ◽  
Laboratory Equipment ◽  
Total N ◽  
Blood Spots ◽  
Stable Source ◽  
Analytical Variation

Abstract Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.

Therapeutic drug monitoring (TDM) of the recent antipsychotic ziprasidone in dried blood spots (DBS) and plasma

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
L Mercolini ◽  
G Fulgenzi ◽  
M Melis ◽  
G Boncompagni ◽  
LJ Albers ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Blood Spots
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