Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking

2022 ◽  
Author(s):  
Yujun Ning ◽  
Lin Wei ◽  
Shen Lin ◽  
Yifan Jiang ◽  
Naidong Wang ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Capsid Proteins ◽  
Viral Capsid ◽  
Upconversion Nanoparticles ◽  
Viral Capsid Proteins

scholarly journals Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher
Keyword(s):  
Fisher Information ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Samples ◽  
Tracking System ◽  
Single Particle Tracking ◽  
Uniform Sampling ◽  
Single Dimension ◽  
3D Localization ◽  
Particle Localization

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

pH-Mediated nanoparticle dynamics in hydrogel nanocomposites

Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto
Keyword(s):  
Quantum Dot ◽  
Ethylene Glycol ◽  
Particle Tracking ◽  
Single Particle ◽  
Silica Particles ◽  
Single Particle Tracking ◽  
Poly Ethylene Glycol ◽  
Hydrogel Nanocomposites ◽  
Poly Ethylene ◽  
Nanoparticle Dynamics

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).

scholarly journals Nonstructural Protein NP1 of Human Bocavirus 1 Plays a Critical Role in the Expression of Viral Capsid Proteins

2016 ◽  
Vol 90 (9) ◽  
pp. 4658-4669 ◽  
Author(s):  
Wei Zou ◽  
Fang Cheng ◽  
Weiran Shen ◽  
John F. Engelhardt ◽  
Ziying Yan ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Capsid Protein ◽  
Nonstructural Protein ◽  
Critical Role ◽  
The Other ◽  
Capsid Proteins ◽  
Viral Capsid ◽  
Human Bocavirus ◽  
Human Airway ◽  
Viral Capsid Proteins

ABSTRACTA novel chimeric parvoviral vector, rAAV2/HBoV1, in which the recombinant adeno-associated virus 2 (rAAV2) genome is pseudopackaged by the human bocavirus 1 (HBoV1) capsid, has been shown to be highly efficient in gene delivery to human airway epithelia (Z. Yan et al., Mol Ther 21:2181–2194, 2013,http://dx.doi.org/10.1038/mt.2013.92). In this vector production system, we used an HBoV1 packaging plasmid, pHBoV1NSCap, that harbors HBoV1 nonstructural protein (NS) and capsid protein (Cap) genes. In order to simplify this packaging plasmid, we investigated the involvement of the HBoV1 NS proteins in capsid protein expression. We found that NP1, a small NS protein encoded by the middle open reading frame, is required for the expression of the viral capsid proteins (VP1, VP2, and VP3). We also found that the other NS proteins (NS1, NS2, NS3, and NS4) are not required for the expression of VP proteins. We performed systematic analyses of the HBoV1 mRNAs transcribed from the pHBoV1NSCap packaging plasmid and its derivatives in HEK 293 cells. Mechanistically, we found that NP1 is required for both the splicing and the read-through of the proximal polyadenylation site of the HBoV1 precursor mRNA, essential functions for the maturation of capsid protein-encoding mRNA. Thus, our study provides a unique example of how a small viral nonstructural protein facilitates the multifaceted regulation of capsid gene expression.IMPORTANCEA novel chimeric parvoviral vector, rAAV2/HBoV1, expressing a full-length cystic fibrosis transmembrane conductance regulator (CFTR) gene, is capable of correcting CFTR-dependent chloride transport in cystic fibrosis human airway epithelium. Previously, an HBoV1 nonstructural and capsid protein-expressing plasmid, pHBoV1NSCap, was used to package the rAAV2/HBoV1 vector, but yields remained low. In this study, we demonstrated that the nonstructural protein NP1 is required for the expression of capsid proteins. However, we found that the other four nonstructural proteins (NS1 to -4) are not required for expression of capsid proteins. By mutating theciselements that function as internal polyadenylation signals in the capsid protein-expressing mRNA, we constructed a simple HBoV1 capsid protein-expressing gene that expresses capsid proteins as efficiently as pHBoV1NSCap does, and at similar ratios, but independently of NP1. Our study provides a foundation to develop a better packaging system for rAAV2/HBoV1 vector production.

Adaptive optics enables three-dimensional single particle tracking at the sub-millisecond scale

2013 ◽  
Vol 102 (17) ◽  
pp. 173702 ◽  
Author(s):  
Manuel F. Juette ◽  
Felix E. Rivera-Molina ◽  
Derek K. Toomre ◽  
Joerg Bewersdorf
Keyword(s):  
Adaptive Optics ◽  
Particle Tracking ◽  
Single Particle ◽  
Three Dimensional ◽  
Single Particle Tracking

scholarly journals Microrheological measurement of microcrystalline cellulose solution using single particle tracking

2018 ◽  
Vol 9 (5S) ◽  
pp. 245
Author(s):  
S.K. Ayop ◽  
S Riyanto ◽  
W.N.S.W. Aziz ◽  
I.R. Mustapa ◽  
Y Munajat
Keyword(s):  
Microcrystalline Cellulose ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Cellulose Solution

scholarly journals Single particle tracking reveals spatial and dynamic organization of theEscherichiacolibiofilm matrix

2014 ◽  
Vol 16 (8) ◽  
pp. 085014 ◽  
Author(s):  
Alona Birjiniuk ◽  
Nicole Billings ◽  
Elizabeth Nance ◽  
Justin Hanes ◽  
Katharina Ribbeck ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Dynamic Organization
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