Synthetic fertilizer is responsible for the greatly increased crop yields that have enabled worldwide industrialization. However, the production and use of such fertilizers are environmentally unfriendly and unsustainable; synthetic fertilizers are produced via non-renewable resources and fertilizer runoff causes groundwater contamination and eutrophication. A promising alternative to synthetic fertilizer is bacterial inoculation. In this process, a symbiotic relationship is formed between a crop and bacteria species that can fix nitrogen, solubilize phosphorus, and stimulate plant hormone production. The bacteria carrier developed here aims to maintain bacteria viability while in storage, protect bacteria while encapsulated, and provide a sustained and controllable bacterial release. This novel bacterial delivery method utilizes inorganic nanomaterials, silica microbeads, to encapsulate symbiotic bacteria. These microbeads, which were produced with aqueous, non-toxic precursors, are sprayed directly onto crop seeds and solidify on the seeds as a resilient silica matrix. The bacterial release from the carrier was found by submerging coated seeds in solution to simulate degradation in soil environments, measuring the number of bacteria released by the plate count technique, and comparing the carrier to seeds coated only in bacteria. The carrier’s effectiveness to enhance plant growth was determined through greenhouse plant assays with alfalfa (<i>Medicago sativa</i>) plants and the nitrogen-fixing <i>Sinorhizobium meliloti</i> Rm1021 strain. When compared to bacteria-only inoculation, the silica microbead carrier exhibited significantly (P < 0.05) increased holding capacity of viable bacteria and increased plant growth by a similar amount, demonstrating the capability of inorganic nanomaterials for microbial delivery. The carrier presented in this work has potential applications for commercial agriculture and presents an opportunity to further pursue more sustainable agricultural practices.
SAFETY AND EFFICACY ASPECTS OF PULSED FIELD ABLATION CATHETERS AS A FUNCTION OF ELECTRODE PROXIMITY TO BLOOD AND ENERGY DELIVERY METHOD
