scholarly journals A general Ca-MOM platform with enhanced acid-base stability for enzyme biocatalysis

2021 ◽  
Author(s):  
Yanxiong Pan ◽  
Qiaobin Li ◽  
Hui Li ◽  
Jasmin Farmakes ◽  
Angel Ugrinov ◽  
...  
2009 ◽  
Vol 44 (12) ◽  
pp. 3098-3111 ◽  
Author(s):  
Zhaoyang Chen ◽  
Zhaokun Luan ◽  
Zhiping Jia ◽  
Xiaosen Li
Keyword(s):  

1978 ◽  
Vol 24 (5) ◽  
pp. 793-795 ◽  
Author(s):  
M C Steiner ◽  
B A Shapiro ◽  
J Kavanaugh ◽  
J R Walton ◽  
W Johnson

Abstract We describe how to prepare, store, and use a hemolyzed blood product for simultaneous pH, pCO2, and pO2 quality control. Tonometry of the blood product with two oxygen and two carbon dioxide concentrations resulted in consistent and reproducible values during 38 weeks. The resulting pH values were consistent and reproducible, demonstrating the metabolic acid-base stability of the blood product. We conclude that the proper preparation, storage, and use of the product results in consistent, reproducible, and economical quality control for pH, pCO2 and pO2 blood measurements.


RSC Advances ◽  
2015 ◽  
Vol 5 (75) ◽  
pp. 61044-61050 ◽  
Author(s):  
Chao Zeng ◽  
Hongxia Wang ◽  
Hua Zhou ◽  
Tong Lin

A self-cleaning, superhydrophobic cotton with excellent washing durability, solvent resistance, and acid/base stability has been prepared using a single-pot solution.


1987 ◽  
Vol 67 (2) ◽  
pp. 427-436 ◽  
Author(s):  
A. L. SCHAEFER ◽  
H. DOORNENBAL ◽  
A. K. W. TONG ◽  
A. C. MURRAY ◽  
A. P. SATHER

In an effort to elucidate physiological factors involved in the development of pale-soft-exudative pork, blood acid base status was assessed in swine from two genetic lines of pigs and their F1 cross. The lines consisted of: (1) pigs that reacted positively (skeletal muscle rigidity) to the respiratory administration of halothane (halothane positive (H+)) based on Pietrain × Lacombe breed crosses, (2) Purebred Lacombe pigs that did not react positively to halothane anesthesia (Lac) and (3) pigs which were the progeny of crossbreeding (C) between halothane positive and negative animals. In addition, time off feed prior to slaughter (0, 24 or 48 h) was imposed as a stressor in order to test response differences among the three lines. The venous blood PCO2, total CO2, bicarbonate ion levels, standard bicarbonate and base excess levels were found to be higher in the H + pigs compared to either Lac or C pigs. All pig lines displayed higher blood pH, total CO2, bicarbonate ion, standard bicarbonate and base excess yet lower PO2 at 24 h off feed compared to 0 h off feed. These data suggest that H+ pigs have a greater tendency toward hypercapnia and a blood base excess than either Lac or C pigs. In addition, the incidence of hypercapnia and blood base excess for H +, Lac and C pigs was greatest at 24 h off feed. Key words: Acid-base stability, pig genotypes, fasting


1986 ◽  
Vol 66 (3) ◽  
pp. 293-302 ◽  
Author(s):  
G.M. Barnas ◽  
M. Gleeson ◽  
W. Rautenberg

2012 ◽  
Vol 134 (48) ◽  
pp. 19524-19527 ◽  
Author(s):  
Sharath Kandambeth ◽  
Arijit Mallick ◽  
Binit Lukose ◽  
Manoj V. Mane ◽  
Thomas Heine ◽  
...  

2020 ◽  
Author(s):  
Xiaohong Yu ◽  
Zhicai Yang ◽  
Keyue Li ◽  
Rui Liu ◽  
Huijie Pei ◽  
...  

Abstract Background: Lactic acid bacteria (LAB) can produce bacteriostatic substances, among which bacteriocins attracted wide attention in food preservation for decades. Up to date, nisin (class I bacteriocins) has been considered to be the only bacteriocin produced by Lactococcus lactis strains for commercial use. Moreover, there are many other reports concerning the isolated bacteriocins for potential application in food industry while some exhibited a narrow bactericidal spectrum, thermal stability and low acid-base stability. Jinhua ham is a representative of traditional dry-cured meat product in China. The microbial community structure and diversity was speculated to be responsible for flavor and quality formation of Jinhua ham, protecting from the spoilage microorganisms contamination. However, no studies was performed to investigate the bacteriocin from LAB in Jinhua ham. Thus, the objective of this study was to screen out high-efficiency, safe and non-toxic bacteriocin-producing lactic acid bacteria (LAB) from Jinhua Ham and subsequently perform the purification, identification and characterization of its bacteriocin. Results: The bacteriocin-producing LAB was screened from Jinhua ham and then designated as Pediococcus pentosaceus Z-1 by colony morphology and 16S rDNA sequencing. The bacteriocin was then crudely extracted from the bacterial cell-free supernatant by pH adsorption, and further purified by cellulose DEAE-52 ion exchange and Sephadex G-50 chromatography columns. The tricine-SDS-PAGE electrophoresis showed a highly purified protein band with 8227.35 Da with 60 amino acids identified by MALDI-TOF-MS analysis. The bacteriocin was named as pediocin Z-1 and its antibacterial activity exhibited an acid-base stability between pH 2-10 and a thermal stability at a range of 50-110°C. The pediocin Z-1 was sensitive to proteases and showed an inhibitory effect against Gram-positive bacteria and Gram-negative bacteria, including Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, Salmonella potsdam and Escherichia coli. Conclusions: Our data suggest that pediocin Z-1 has a comparable effect of antibacterial activity with nisin and it is promising that pediocin Z-1 has great potential for application prospect in the food industry.


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