scholarly journals Sea cucumber Holothuria polii (Delle Chiaje, 1823) as new model for embryo bioassays in ecotoxicological studies

Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124819 ◽  
Author(s):  
Lorenzo Morroni ◽  
Arnold Rakaj ◽  
Luca Grosso ◽  
Alessandra Fianchini ◽  
David Pellegrini ◽  
...  
2018 ◽  
Vol 6 ◽  
pp. 205031211880954 ◽  
Author(s):  
Mike Kareh ◽  
Rana El Nahas ◽  
Lamis Al-Aaraj ◽  
Sara Al-Ghadban ◽  
Nataly Naser Al Deen ◽  
...  

Objective: Sea cucumbers are considered among the most important functional foods. Following bioassay guided fractionation, we assessed the anti-proliferative and anti-inflammatory activities of Holothuria polii ( H. polii) extracts. Methods: Sea cucumber ethanolic extract and the partially purified aqueous fractions were assessed for their anti-proliferative activities. These latter bioactivities were evaluated in the highly invasive MDA-MB-231 human breast cancer cells in two-dimensional and three-dimensional cultures using trypan blue exclusion assay. The tumor-suppressive effects of sea cucumber ethanolic extract and aqueous fractions were assayed by measuring the trans-well invasion of MDA-MB-231 cells and the expression of some epithelial mesenchymal transition markers using quantitative reverse-transcription polymerase chain reaction and western blot analysis. The anti-inflammatory activity of the aqueous fraction was tested by measuring the secreted levels of interleukin-6, nitric oxide, and matrix metalloproteinase 9 in endotoxin-induced mammary epithelial SCp2 cells and interleukin-1β in phorbol-12-myristate-13-acetate-activated human monocytic THP-1 cells. Results: Sea cucumber ethanolic extract and the aqueous fraction significantly decreased the proliferation of MDA-MB-231 cells by more than 50% at similar and noncytotoxic concentrations and caused an arrest in the S-phase of the cell cycle of treated cells. In contrast, petroleum ether, chloroform, ethyl acetate, and n-butanol organic fractions did not show any significant activity. Furthermore, sea cucumber ethanolic extract and aqueous fraction reduced the proliferation of MDA-MB-231 cells in three-dimensional cultures by more than 60% at noncytotoxic concentrations. In addition, treatment with these concentrations resulted in the loss of stellate outgrowths in favor of spherical aggregates and a 30% decrease in invasive properties. Both sea cucumber ethanolic extract and aqueous decreased the transcription of vimentin and the protein expression levels of vimentin and N-cadherin in three-dimensional cultures. The aqueous fraction decreased the levels of inflammatory markers interleukin-6, nitric oxide, and matrix metalloproteinase 9 in the mouse mammary SCp2 cells, and the level of interleukin-1β produced by phorbol-12-myristate-13-acetate-activated THP-1 human monocytic cells. Conclusion: The data reveal for the first time promising anti-proliferative and anti-inflammatory activities in H. polii water extract in two-dimensional and three-dimensional culture models.


2019 ◽  
Vol 273 ◽  
pp. 197737 ◽  
Author(s):  
L. Nerva ◽  
M. Forgia ◽  
M. Ciuffo ◽  
W. Chitarra ◽  
M. Chiapello ◽  
...  

2008 ◽  
Vol 18 (1) ◽  
pp. 23-26 ◽  
Author(s):  
H. Ismail ◽  
S. Lemriss ◽  
Z. Ben Aoun ◽  
L. Mhadhebi ◽  
A. Dellai ◽  
...  

2019 ◽  
Vol 121 ◽  
pp. 1145-1153 ◽  
Author(s):  
Mohamed Ben Mansour ◽  
Rafik Balti ◽  
Lamia Yacoubi ◽  
Véronique Ollivier ◽  
Frédéric Chaubet ◽  
...  

1958 ◽  
Vol 13 (6) ◽  
pp. 404-407 ◽  
Author(s):  
Rudolf K. Zahn ◽  
Annesuse Docter ◽  
Hanskurt Müller

A method which serves to isolate the gonads from the sea cucumber (Holothuria polii) is outlined.Criteria that will secure a well determined status of maturity of the sperm are given.From this preparation a deoxyribonucleic acid is made, purified and analysed.It is concluded that the analytical data are in compliance with the theory of Crick and Watson.The ratio of Moles for this DNA while its nitrogen to phosphorus ratio on weight basis is 1,67.


2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Natalia Montero ◽  
Marco Atzori ◽  
Barbara Marras ◽  
Alexandre Bettoschi ◽  
Pierpaolo Nurchis ◽  
...  

Sea cucumbers represent an important part of the diet in Asian and Pacific regions and are also used in traditional medicine. These habits have led to the overexploitation of local sea cucumber populations in these areas, driving the pursuit of new stock regions, such as Mediterranean areas. In Italy, contrarily to that observed for other Mediterranean countries, the exploitation of sea cucumber stocks is not extensive, which opens a new market opportunity. Thus, from a food safety perspective, this work aims at reporting the first assessment of trace metal concentrations (As, Cd, Cr, Cu, Hg, Ni, Pb) in the edible tissues of Holothuria polii and Holoturia tubulosa collected in Sardinia, the second-largest island in the Mediterranean Sea. Metal concentrations found in H. polii were generally higher than in H. tubulosa. However, in both species, they were lower than those reported for other areas of the Western Mediterranean. Cd, Hg, and Pb were below the limits established for seafood in Europe. As concentrations were in the range of those measured in other commercial seafood species in the Mediterranean. Thus, these species may be harvested and traded to fulfil the demands of local and international markets.


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