One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography

2007 ◽  
Vol 1140 (1-2) ◽  
pp. 107-111 ◽  
Author(s):  
Ming Gu ◽  
Xiaolei Wang ◽  
Zhiguo Su ◽  
Fan Ouyang
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Separation And Purification ◽  
Salvia Miltiorrhiza Bunge ◽  
Salvianolic Acid ◽  
One Step ◽  
Counter Current

Separation of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography using stepwise elution

2000 ◽  
Vol 904 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Guilian Tian ◽  
Yabin Zhang ◽  
Tianyou Zhang ◽  
Fuquan Yang ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Counter Current

Quantitative determination and validation of four phenolic acids in Salvia Miltiorrhiza Bunge using 1H-NMR spectroscopy

2019 ◽  
Vol 16 ◽  
Author(s):  
Zhuoni Hou ◽  
Zongsuo Liang ◽  
Yuanyuan Li ◽  
Feng Su ◽  
Jipeng Chen ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
1H Nmr Spectroscopy ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Salvia Miltiorrhiza Bunge ◽  
Salvianolic Acid ◽  
Root Extracts

Background: Although chromatography and spectrometry based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts. Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy. Method: Fresh SMB root were extracted using a 70% methanol aqueous solution and quantitatively analysed for danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B using 1H-NMR spectroscopy. Different internal standards were compared and the results were validated using high performance liquid chromatography. Results: The established method was accurate and precise with good recovery. The LOD and LOQ indicated the excellent sensitivity of the method. The robustness was testified by modification of four different parameters, and the differences among each parameter were all less than 2%. Conclusion: qNMR offers a fast, reliable and accurate method of identifying and quantifying danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B in fresh SMB root extracts.

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